This study evaluated the effects of pomegranate (Punica granatum L.) seed essential oil (PSO) on chitosan-based films. The results showed that the addition of PSO slightly increased thickness, while significantly decreasing the moisture content, and solubility properties. The color values of the composite films containing PSO changed noticeably, with a tendency toward light brown, which was beneficial in resisting food decomposition caused by ultraviolet light. X-Ray diffraction analysis (XRD) and Fourier-transform Infrared (FT-IR) results indicated that the addition of PSO affected the structure of the chitosan films, while the interaction between chitosan and polyphenols in PSO established new hydrogen bonds. Scanning electron microscopy (SEM) showed that the surface of the PSO-containing blend films was rougher compared to the chitosan control film, and the roughness increased as the PSO content in the blend films increased. Additionally, composite films that contained PSO had substantial antibacterial action, particularly against pathogenic E. coli. In short, the novel active chitosan-based films with incorporated PSO present broad application prospects in the packaging of fresh-cut meat or vegetables. Therefore, this study will also be beneficial in these areas.
Adiba, A., Razouk, R., Haddioui, A., Ouaabou, R., Hamdani, A., Kouighat, M., & Hssaini, L. (2023). FTIR spectroscopy-based lipochemical fingerprints involved in pomegranate response to water stress. Heliyon, 9(6), e16687. https://doi.org/10.1016/j.heliyon.2023.e16687
Bajić, M., Ročnik, T., Oberlintner, A., Scognamiglio, F., Novak, U., & Likozar, B. (2019). Natural plant extracts as active components in chitosan-based films: A comparative study. Food Packaging and Shelf Life, 21, 100365. https://doi.org/10.1016/j.fpsl.2019.100365
Bof, M. J., Jiménez, A., Locaso, D. E., García, M. A., & Chiralt, A. (2016). Grapefruit Seed Extract and Lemon Essential Oil as Active Agents in Corn Starch–Chitosan Blend Films. Food and Bioprocess Technology, 9(12), 2033–2045. https://doi.org/10.1007/s11947-016-1789-8
Eroglu, E., & Girgin, S. N. (2021). A unique phenolic extraction method from olive oil macerate of Hypericum perforatum using DMSO: Assessment of in vitro anticancer activity, LC-MS/MS profile, total phenolic content and antioxidant capacity. South African Journal of Botany, 139, 6–11. https://doi.org/10.1016/j.sajb.2021.01.015
Evcil, M., & Karakaplan, M. (2022). Salicylaldehydediol Grafted onto Chitosan: Characterization and Their Film Properties. Journal of the Turkish Chemical Society Section A: Chemistry, 9(3), 879–888. https://doi.org/10.18596/jotcsa.1084225
Ghaffari, A., Navaee, K., Oskoui, M., Bayati, K., & Rafiee-Tehrani, M. (2007). Preparation and characterization of free mixed-film of pectin/chitosan/Eudragit® RS intended for sigmoidal drug delivery. European Journal of Pharmaceutics and Biopharmaceutics, 67(1), 175–186. https://doi.org/10.1016/j.ejpb.2007.01.013
Hafsa, J., Smach, M. ali, Ben Khedher, M. R., Charfeddine, B., Limem, K., Majdoub, H., & Rouatbi, S. (2016). Physical, antioxidant and antimicrobial properties of chitosan films containing Eucalyptus globulus essential oil. LWT - Food Science and Technology, 68, 356–364. https://doi.org/10.1016/j.lwt.2015.12.050
Haghighi, H., Biard, S., Bigi, F., De Leo, R., Bedin, E., Pfeifer, F., … Pulvirenti, A. (2019). Comprehensive characterization of active chitosan-gelatin blend films enriched with different essential oils. Food Hydrocolloids, 95, 33–42. https://doi.org/10.1016/j.foodhyd.2019.04.019
Hosseini, S. F., Ghaderi, J., & Gómez-Guillén, M. C. (2021). trans-Cinnamaldehyde-doped quadripartite biopolymeric films: Rheological behavior of film-forming solutions and biofunctional performance of films. Food Hydrocolloids, 112, 106339. https://doi.org/10.1016/j.foodhyd.2020.106339
K., R., G., B., Banat, F., Show, P. L., & Cocoletzi, H. H. (2019). Mango leaf extract incorporated chitosan antioxidant film for active food packaging. International Journal of Biological Macromolecules, 126, 1234–1243. https://doi.org/10.1016/j.ijbiomac.2018.12.196
Kanmani, P., & Rhim, J.-W. (2014). Properties and characterization of bionanocomposite films prepared with various biopolymers and ZnO nanoparticles. Carbohydrate Polymers, 106, 190–199. https://doi.org/10.1016/j.carbpol.2014.02.007
Khezrian, A., & Shahbazi, Y. (2018). Application of nanocompostie chitosan and carboxymethyl cellulose films containing natural preservative compounds in minced camel’s meat. International Journal of Biological Macromolecules, 106, 1146–1158. https://doi.org/10.1016/j.ijbiomac.2017.08.117
Khoddami, A., Man, Y. B. C., & Roberts, T. H. (2014). Physico‐chemical properties and fatty acid profile of seed oils from pomegranate ( Punica granatum L.) extracted by cold pressing. European Journal of Lipid Science and Technology, 116(5), 553–562. https://doi.org/10.1002/ejlt.201300416
Li, Z., Lin, S., An, S., Liu, L., Hu, Y., & Wan, L. (2019). Preparation, characterization and anti-aflatoxigenic activity of chitosan packaging films incorporated with turmeric essential oil. International Journal of Biological Macromolecules, 131, 420–434. https://doi.org/10.1016/j.ijbiomac.2019.02.169
Liu, J., Meng, C., Yan, Y., Shan, Y., Kan, J., & Jin, C. (2016). Protocatechuic acid grafted onto chitosan: Characterization and antioxidant activity. International Journal of Biological Macromolecules, 89, 518–526. https://doi.org/10.1016/j.ijbiomac.2016.04.089
Liu, T., Liu, L., Gong, X., Chi, F., & Ma, Z. (2021). Fabrication and comparison of active films from chitosan incorporating different spice extracts for shelf life extension of refrigerated pork. LWT, 135, 110181. https://doi.org/10.1016/j.lwt.2020.110181
Liu, T., Wang, J., Chi, F., Tan, Z., & Liu, L. (2020). Development and Characterization of Novel Active Chitosan Films Containing Fennel and Peppermint Essential Oils. Coatings, 10(10), 936. https://doi.org/10.3390/coatings10100936
McClements, D. J. (2004). Food Emulsions. https://doi.org/10.1201/9781420039436
Mohammadi, M., Mirabzadeh, S., Shahvalizadeh, R., & Hamishehkar, H. (2020). Development of novel active packaging films based on whey protein isolate incorporated with chitosan nanofiber and nano-formulated cinnamon oil. International Journal of Biological Macromolecules, 149, 11–20. https://doi.org/10.1016/j.ijbiomac.2020.01.083
Mouhoub, A., Raouan, S. E., Guendouz, A., El Alaoui-Talibi, Z., Koraichi, S. I., El Abed, S., … El Modafar, C. (2022). Antiadhesion effect of the chitosan-based film incorporated with essential oils against foodborne bacteria. Industrial Crops and Products, 189, 115742. https://doi.org/10.1016/j.indcrop.2022.115742
Nguyen, L. T. T., Nguyen, T. T., Nguyen, H. N., & Bui, Q. T. P. (2020). Simultaneous determination of active compounds in Piper betle Linn. leaf extract and effect of extracting solvents on bioactivity. Engineering Reports, 2(10). https://doi.org/10.1002/eng2.12246
Pacheco, N., Naal-Ek, M. G., Ayora-Talavera, T., Shirai, K., Román-Guerrero, A., Fabela-Morón, M. F., & Cuevas-Bernardino, J. C. (2019). Effect of bio-chemical chitosan and gallic acid into rheology and physicochemical properties of ternary edible films. International Journal of Biological Macromolecules, 125, 149–158. https://doi.org/10.1016/j.ijbiomac.2018.12.060
Paul, A., & Radhakrishnan, M. (2020). Pomegranate seed oil in food industry: Extraction, characterization, and applications. Trends in Food Science & Technology, 105, 273–283. https://doi.org/10.1016/j.tifs.2020.09.014
Rauf, A., Imran, M., Abu-Izneid, T., Iahtisham-Ul-Haq, Patel, S., Pan, X., … Rasul Suleria, H. A. (2019). Proanthocyanidins: A comprehensive review. Biomedicine & Pharmacotherapy, 116, 108999. https://doi.org/10.1016/j.biopha.2019.108999
Rempe, C. S., Burris, K. P., Lenaghan, S. C., & Stewart, C. N. (2017). The Potential of Systems Biology to Discover Antibacterial Mechanisms of Plant Phenolics. Frontiers in Microbiology, 8. https://doi.org/10.3389/fmicb.2017.00422
Silva, A., Silva, V., Igrejas, G., Gaivão, I., Aires, A., Klibi, N., … Poeta, P. (2021). Valorization of Winemaking By-Products as a Novel Source of Antibacterial Properties: New Strategies to Fight Antibiotic Resistance. Molecules, 26(8), 2331. https://doi.org/10.3390/molecules26082331
Wali, A. F., Al Dhaheri, Y., Ramakrishna Pillai, J., Mushtaq, A., Rao, P. G. M., Rabbani, S. A., … Farraj, D. A. Al. (2020). LC-MS Phytochemical Screening, In Vitro Antioxidant, Antimicrobial and Anticancer Activity of Microalgae Nannochloropsis oculata Extract. Separations, 7(4), 54. https://doi.org/10.3390/separations7040054
Wang, L.-F., & Rhim, J.-W. (2015). Preparation and application of agar/alginate/collagen ternary blend functional food packaging films. International Journal of Biological Macromolecules, 80, 460–468. https://doi.org/10.1016/j.ijbiomac.2015.07.007
Xue, F., Zhao, M., Liu, X., Chu, R., Qiao, Z., Li, C., & Adhikari, B. (2021). Physicochemical properties of chitosan/zein/essential oil emulsion-based active films functionalized by polyphenols. Future Foods, 3, 100033. https://doi.org/10.1016/j.fufo.2021.100033
Yang, Z., Li, L., Chen, C., Zhang, Y., Yang, Y., Zhang, P., & Bao, G. (2022). Chemical composition and antibacterial activity of 12 medicinal plant ethyl acetate extracts using LC–MS feature‐based molecular networking. Phytochemical Analysis, 33(3), 473–489. https://doi.org/10.1002/pca.3103
Year 2024,
Volume: 8 Issue: 2, 261 - 272, 27.06.2024
Adiba, A., Razouk, R., Haddioui, A., Ouaabou, R., Hamdani, A., Kouighat, M., & Hssaini, L. (2023). FTIR spectroscopy-based lipochemical fingerprints involved in pomegranate response to water stress. Heliyon, 9(6), e16687. https://doi.org/10.1016/j.heliyon.2023.e16687
Bajić, M., Ročnik, T., Oberlintner, A., Scognamiglio, F., Novak, U., & Likozar, B. (2019). Natural plant extracts as active components in chitosan-based films: A comparative study. Food Packaging and Shelf Life, 21, 100365. https://doi.org/10.1016/j.fpsl.2019.100365
Bof, M. J., Jiménez, A., Locaso, D. E., García, M. A., & Chiralt, A. (2016). Grapefruit Seed Extract and Lemon Essential Oil as Active Agents in Corn Starch–Chitosan Blend Films. Food and Bioprocess Technology, 9(12), 2033–2045. https://doi.org/10.1007/s11947-016-1789-8
Eroglu, E., & Girgin, S. N. (2021). A unique phenolic extraction method from olive oil macerate of Hypericum perforatum using DMSO: Assessment of in vitro anticancer activity, LC-MS/MS profile, total phenolic content and antioxidant capacity. South African Journal of Botany, 139, 6–11. https://doi.org/10.1016/j.sajb.2021.01.015
Evcil, M., & Karakaplan, M. (2022). Salicylaldehydediol Grafted onto Chitosan: Characterization and Their Film Properties. Journal of the Turkish Chemical Society Section A: Chemistry, 9(3), 879–888. https://doi.org/10.18596/jotcsa.1084225
Ghaffari, A., Navaee, K., Oskoui, M., Bayati, K., & Rafiee-Tehrani, M. (2007). Preparation and characterization of free mixed-film of pectin/chitosan/Eudragit® RS intended for sigmoidal drug delivery. European Journal of Pharmaceutics and Biopharmaceutics, 67(1), 175–186. https://doi.org/10.1016/j.ejpb.2007.01.013
Hafsa, J., Smach, M. ali, Ben Khedher, M. R., Charfeddine, B., Limem, K., Majdoub, H., & Rouatbi, S. (2016). Physical, antioxidant and antimicrobial properties of chitosan films containing Eucalyptus globulus essential oil. LWT - Food Science and Technology, 68, 356–364. https://doi.org/10.1016/j.lwt.2015.12.050
Haghighi, H., Biard, S., Bigi, F., De Leo, R., Bedin, E., Pfeifer, F., … Pulvirenti, A. (2019). Comprehensive characterization of active chitosan-gelatin blend films enriched with different essential oils. Food Hydrocolloids, 95, 33–42. https://doi.org/10.1016/j.foodhyd.2019.04.019
Hosseini, S. F., Ghaderi, J., & Gómez-Guillén, M. C. (2021). trans-Cinnamaldehyde-doped quadripartite biopolymeric films: Rheological behavior of film-forming solutions and biofunctional performance of films. Food Hydrocolloids, 112, 106339. https://doi.org/10.1016/j.foodhyd.2020.106339
K., R., G., B., Banat, F., Show, P. L., & Cocoletzi, H. H. (2019). Mango leaf extract incorporated chitosan antioxidant film for active food packaging. International Journal of Biological Macromolecules, 126, 1234–1243. https://doi.org/10.1016/j.ijbiomac.2018.12.196
Kanmani, P., & Rhim, J.-W. (2014). Properties and characterization of bionanocomposite films prepared with various biopolymers and ZnO nanoparticles. Carbohydrate Polymers, 106, 190–199. https://doi.org/10.1016/j.carbpol.2014.02.007
Khezrian, A., & Shahbazi, Y. (2018). Application of nanocompostie chitosan and carboxymethyl cellulose films containing natural preservative compounds in minced camel’s meat. International Journal of Biological Macromolecules, 106, 1146–1158. https://doi.org/10.1016/j.ijbiomac.2017.08.117
Khoddami, A., Man, Y. B. C., & Roberts, T. H. (2014). Physico‐chemical properties and fatty acid profile of seed oils from pomegranate ( Punica granatum L.) extracted by cold pressing. European Journal of Lipid Science and Technology, 116(5), 553–562. https://doi.org/10.1002/ejlt.201300416
Li, Z., Lin, S., An, S., Liu, L., Hu, Y., & Wan, L. (2019). Preparation, characterization and anti-aflatoxigenic activity of chitosan packaging films incorporated with turmeric essential oil. International Journal of Biological Macromolecules, 131, 420–434. https://doi.org/10.1016/j.ijbiomac.2019.02.169
Liu, J., Meng, C., Yan, Y., Shan, Y., Kan, J., & Jin, C. (2016). Protocatechuic acid grafted onto chitosan: Characterization and antioxidant activity. International Journal of Biological Macromolecules, 89, 518–526. https://doi.org/10.1016/j.ijbiomac.2016.04.089
Liu, T., Liu, L., Gong, X., Chi, F., & Ma, Z. (2021). Fabrication and comparison of active films from chitosan incorporating different spice extracts for shelf life extension of refrigerated pork. LWT, 135, 110181. https://doi.org/10.1016/j.lwt.2020.110181
Liu, T., Wang, J., Chi, F., Tan, Z., & Liu, L. (2020). Development and Characterization of Novel Active Chitosan Films Containing Fennel and Peppermint Essential Oils. Coatings, 10(10), 936. https://doi.org/10.3390/coatings10100936
McClements, D. J. (2004). Food Emulsions. https://doi.org/10.1201/9781420039436
Mohammadi, M., Mirabzadeh, S., Shahvalizadeh, R., & Hamishehkar, H. (2020). Development of novel active packaging films based on whey protein isolate incorporated with chitosan nanofiber and nano-formulated cinnamon oil. International Journal of Biological Macromolecules, 149, 11–20. https://doi.org/10.1016/j.ijbiomac.2020.01.083
Mouhoub, A., Raouan, S. E., Guendouz, A., El Alaoui-Talibi, Z., Koraichi, S. I., El Abed, S., … El Modafar, C. (2022). Antiadhesion effect of the chitosan-based film incorporated with essential oils against foodborne bacteria. Industrial Crops and Products, 189, 115742. https://doi.org/10.1016/j.indcrop.2022.115742
Nguyen, L. T. T., Nguyen, T. T., Nguyen, H. N., & Bui, Q. T. P. (2020). Simultaneous determination of active compounds in Piper betle Linn. leaf extract and effect of extracting solvents on bioactivity. Engineering Reports, 2(10). https://doi.org/10.1002/eng2.12246
Pacheco, N., Naal-Ek, M. G., Ayora-Talavera, T., Shirai, K., Román-Guerrero, A., Fabela-Morón, M. F., & Cuevas-Bernardino, J. C. (2019). Effect of bio-chemical chitosan and gallic acid into rheology and physicochemical properties of ternary edible films. International Journal of Biological Macromolecules, 125, 149–158. https://doi.org/10.1016/j.ijbiomac.2018.12.060
Paul, A., & Radhakrishnan, M. (2020). Pomegranate seed oil in food industry: Extraction, characterization, and applications. Trends in Food Science & Technology, 105, 273–283. https://doi.org/10.1016/j.tifs.2020.09.014
Rauf, A., Imran, M., Abu-Izneid, T., Iahtisham-Ul-Haq, Patel, S., Pan, X., … Rasul Suleria, H. A. (2019). Proanthocyanidins: A comprehensive review. Biomedicine & Pharmacotherapy, 116, 108999. https://doi.org/10.1016/j.biopha.2019.108999
Rempe, C. S., Burris, K. P., Lenaghan, S. C., & Stewart, C. N. (2017). The Potential of Systems Biology to Discover Antibacterial Mechanisms of Plant Phenolics. Frontiers in Microbiology, 8. https://doi.org/10.3389/fmicb.2017.00422
Silva, A., Silva, V., Igrejas, G., Gaivão, I., Aires, A., Klibi, N., … Poeta, P. (2021). Valorization of Winemaking By-Products as a Novel Source of Antibacterial Properties: New Strategies to Fight Antibiotic Resistance. Molecules, 26(8), 2331. https://doi.org/10.3390/molecules26082331
Wali, A. F., Al Dhaheri, Y., Ramakrishna Pillai, J., Mushtaq, A., Rao, P. G. M., Rabbani, S. A., … Farraj, D. A. Al. (2020). LC-MS Phytochemical Screening, In Vitro Antioxidant, Antimicrobial and Anticancer Activity of Microalgae Nannochloropsis oculata Extract. Separations, 7(4), 54. https://doi.org/10.3390/separations7040054
Wang, L.-F., & Rhim, J.-W. (2015). Preparation and application of agar/alginate/collagen ternary blend functional food packaging films. International Journal of Biological Macromolecules, 80, 460–468. https://doi.org/10.1016/j.ijbiomac.2015.07.007
Xue, F., Zhao, M., Liu, X., Chu, R., Qiao, Z., Li, C., & Adhikari, B. (2021). Physicochemical properties of chitosan/zein/essential oil emulsion-based active films functionalized by polyphenols. Future Foods, 3, 100033. https://doi.org/10.1016/j.fufo.2021.100033
Yang, Z., Li, L., Chen, C., Zhang, Y., Yang, Y., Zhang, P., & Bao, G. (2022). Chemical composition and antibacterial activity of 12 medicinal plant ethyl acetate extracts using LC–MS feature‐based molecular networking. Phytochemical Analysis, 33(3), 473–489. https://doi.org/10.1002/pca.3103
Evcil, M. (2024). Production of a novel biodegradable film made from chitosan and pomegranate (Punica granatum L.) seed essential oil. International Journal of Agriculture Environment and Food Sciences, 8(2), 261-272. https://doi.org/10.31015/jaefs.2024.2.3
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