Improving seed germination and bulb induction of Allium tuncelianum kolmann under aseptic conditions

Year 2024, Volume: 8 Issue: 4, 913 - 918, 28.12.2024

Süleyman Kızıl , Khalid Mahmood Khawar

https://doi.org/10.31015/jaefs.2024.4.21

Abstract

Allium tuncelianum (Kollman) N. Ozhatay, B. Mathew & Siraneci] or Tunceli garlic is endemic to the Eastern Turkish Provinces of Tunceli, Sivas Erzincan and [Munzur mountains]. They are edible and bear attractive deep lilac colored flowers with fertile black deep dormant seeds. Tunceli garlic seeds were collected from field-grown plants and aimed to break seed dormancy to optimize conditions for induction of bulblets, along with their growth, development, and increased bulb diameter. Therefore, these were cultured on MS medium amended with different strengths of KNO3. They were germinated on MS medium with or without 20 g L-1 sucrose followed by their culture on 1, 2, 4 and 6 × KNO3 (found in MS medium) to increase bulb diameter. Improved seed germination was noted on MS medium with and without sucrose but with variation compared to the previous reports. The bulb formation rate on each of the germinated seeds was not parallel. The results showed 34 and 28.5% bulb induction noted on germinated seeds after 150 and 158 days on MS medium containing 20 g L-1 sucrose and no sucrose in the same sequence. The results emphatically noted the role of cold stratification on agar-solidified MS medium supplemented with sucrose to improve seed germination. The best increase in bulb diameter was noted on MS medium containing 1 × KNO3 (found in MS medium) after 178 days with bulblet diameter and weight of 0.54 cm and 0.048 g, respectively. Consequently, the bulbs induced on sucrose-containing MS medium could be transferred to pots earlier. Increased (>1 × KNO3 found in MS medium) negatively affected on the growth and development of Tunceli garlic bulbs. The strategy of seed germination and bulblet induction reported in this study could be positively used to conserve and protect this endemic plant species.

Keywords

Tunceli garlic, Seed, dormancy, Bulblets, Bulb growth

Ethical Statement

The article was previously presented as a conference paper at the XX International Botanical Congress held in Madrid, Spain. The abstract of the article has been published, but the full text has not been released.

References

• Arguello, J.A., Falcon, L.R., Seisdedos, L., et al. (2001). Morphological changes in garlic (Allium sativum L.) microbulblets during dormancy and sprouting as related to peroxidase activity and gibberellin GA3 content. Biocell 25: 1–9. PMID: 11387870.
• Arslan, N., Ipek, A., Sarihan, E.O. (2010). Effects of Different Bulb Circumferences on Yield and Some Characteristics of Tunceli Garlic (Allium tuncelianum). XIX. International Symposium on Plant Originated Crude Drugs. Mersin, Turkey, Proceeding Book, 358-365. (in Turkish)
• Baktir, I. (2005). In vitro propagation of Tunceli garlic (Allium tuncelianum)” In: Proceeding of GAP IV. Agricultural Congress, Sanliurfa, Turkey, 206–208. (in Turkish)
• Cantwell, M.I., Kang, J., Hong, G. (2003). Heat treatments control sprouting and rooting of garlic cloves. Postharvest Biology and Technology 30: 57–65. https://doi.org/10.1016/S0925-5214(03)00060-7
• Dufoo – Hurtado, M.D., Zavala, K.G., Gutierrez, C.M., et al. (2013). Low temperature conditioning of “seed” cloves enhances the expression of phenolic metabolism related genes and anthocyanin content in Coreano garlic (Allium sativum) during plant development. Journal of Agricultural and Food Chemistry 61: 10439–10446. https://doi.org/10.1021/jf403019t
• Etoh, T., Simon, P.W. (2002). Diversity, Fertility and Seed Production of Garlic. In: Allium: Crop Sciences: Recent Advances, Rabinowitch, H. D., and Currah, L., Eds., CABI publishing, 101-117.
• Fulton, T.A., Hall, A.J., Catley, J.L. (2001). Chilling requirements of Paeonia cultivars. Scientia Horticulturae 89: 237–248. https://doi.org/10.1016/S0304-4238(00)00237-5
• Hapkins, W.G. (1999). Introduction to plant physiology. 1- 2, John Wiley and Sons, New York.
• Iciek, M., Kwiecien, I., Wlodek, L. (2009). Biological properties of garlic and garlic-derived organosulfur compounds. Environmental and Molecular Mutagenesis, 50: 247-265. https://doi.org/10.1002/em.20474
• Jacob, C. (2006). A scent of therapy: pharmacological implications of natural products containing redox-active sulfur atoms. Natural Product Reports, 23: 851-863. https://doi.org/10.1039/B609523M
• Kizil, S., Icgil, D.Y., Khawar, K.M. (2014). Improved In vitro regeneration and propagation of Tunceli garlic (Allium tuncelianum L.) from sectioned garlic cloves, leaves and root explants. Journal of Horticultural Science and Biotechnology, 89: 408-414. https://doi.org/10.1080/14620316.2014.11513099
• Kumar, S., Kashyap, M., Sharma, D.R. (2005). In vitro regeneration and bulblet growth from lily bulb scale explants as affected by retardants, sucrose and irradiance. Biologia Plantarum, 49: 629-632.
• Langens – Grrits, M.M., Miller, W.B., Croes, A.F., DeKlerk, G.J. (2003). Effects of low temperature on dormancy breaking and growth after planting in lily bulblets regenerated in vitro. Plant Growth Regulation 40: 267–275. https://doi.org/10.1023/A:1025018728178
• Miller, L.A., Peters. J. (2010). AOSA/SCST Tetrazolium Testing Handbook. Edition: Association of Official Seed Analysts and the Society of Commercial Seed Technologists.
• Munchberg, U., Anwar, A., Mecklenburg, S., Jacob, C. (2007). Polysulfides as biologically active ingredients of garlic. Organic & Biomolecular Chemistry, 5: 1505-1518. https://doi.org/10.1039/B703832A
• Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473-497.
• Ozkan, O., Gul, S., Kart, A., et al. (2013). In vitro antimutagenicity of Allium tuncelianum ethanol extract against induction of chromosome aberration by mutagenic agent mitomycine C. Journal of The Faculty of Veterinary Medicine, Kafkas University, 19: 259-262. DOI: 10.9775/kvfd.2012.7637
• Pooler, M.R., Simon, P.W. (1994). True seed production in garlic. Sexual Plant Reproduction 7: 282-286.
• Rahim, M.A, Fordham, R. (2001). Environmental manipulation for controlling bulbing in garlic. In II International Symposium on Edible Alliaceae. Acta Hortic., 555: 180–188.
• Rahman, H., Shahidul, M.H., Ahmed, M. (2003). Pre-planting temperature treatments for breaking dormancy of garlic cloves. Asian Journal of Plant Sciences 2: 123-126.
• Sosnoskie, L.M., Cardina, J. (2009). Laboratory methods for breaking dormancy in garlic mustard (Alliaria petiolata) seeds. Invasive Plant Science and Management 2: 185-289. https://doi.org/10.1614/IPSM-08-126.1
• Vazquez – Barrios, M.E., Lopez – Echevarria, G., Mercado, E., et al. (2006). Study and prediction of quality changes in garlic cv. Perla (Allium sativum L.) stored at different temperatures. Scientia Horticulturae 108: 127–132. https://doi.org/10.1016/j.scienta.2006.01.013
• Volk, G.M. (2009). Phenotypic characteristics of ten garlic cultivars grown at different North American locations. HortScience 44: 1238–1247. https://doi.org/10.21273/HORTSCI.44.5.1238
• Yanmaz, R., Ermis, S. (2005). Researches on Overcoming the Germination Problems of Tunceli Garlic Seeds (Allium tuncelianum (Kollman), N. Ozhatay, D. Matthew, Siraneci). Turkey 2th Seed Congress, Adana, Turkey, 101-106. (in Turkish)
• Youngblood, A. (2008). Rhamnus L. Buckthorn. US Department of Agriculture Woody Plant Seed Manual: USDA FS Agriculture Handbook 727 General Printing Office, 939-942.