Dimethoate ve Malathion Pestisitlerinin Dreissena polymorpha (Pallas, 1771) Üzerindeki Kombine Etkisinin Sitokrom P450 ve Asetilkolinesteraz Kullanılarak Belirlenmesi

Yıl 2022, , 417 - 424, 31.12.2022

Nuran Cikcikoglu Yildirim , Osman Serdar , Numan Yıldırım

https://doi.org/10.35229/jaes.1168122

Öz

Bu çalışmada, Dreissena polymorpha’da organofosforlu dimethoate ve malathion etken maddeli insektisit karışımlarının toksisitesini ortaya çıkarmak için sitokrom P450 (CYP1A1) ve Asetilkolinesteraz (AchE) aktiviteleri ölçülmüştür. Dimethoate ve malathion'un öldürücü konsantrasyonu (LC50) 96 saat maruziyet sonunda 40,82±2,54 mg/L olarak hesaplanmıştır. D. polymorpha dimethoate ve malathionun üç subletal konsantrasyonuna (LC50 değerine 1/16, 1/8 ve 1/4 oranla) 24 saat ve 96 saat boyunca maruz bırakılmıştır. CYP1A1 ve AChE seviyeleri, ticari kit kullanılarak mikroplaka okuyucuda ölçülmüştür. AChE aktivitelerinin kontrol grubuna göre 96 saat sonra tüm maruziyet gruplarında düştüğü gözlenmiştir. Maruziyet süreleri karşılaştırıldığında 96. saat sonunda enzim aktivitelerinde istatistiksel olarak anlamlı bir değişiklik olmadığı belirlenmiştir. Tüm gruplarda 24 saatin sonunda CYP1A1 seviyelerinde istatistiksel olarak anlamlı bir değişiklik olmamıştır, ancak kontrol grubuna kıyasla 96 saat sonra azalma görülmüştür (p0,05). Dimethoate ve malathion kombinasyonunun toksik yanıtı konsantrasyonlarına bağlı olarak değişebildiği tespit edilmiştir. Sonuç olarak, dimethoate ve malathion karışımlarının, D. polymorpha'da AChE ve CYP1A1 aktivitelerini inhibe ettiği ve bu enzimlerin etkili bir biyobelirteç olarak kullanılabileceği ortaya konmuştur.

Anahtar Kelimeler

AChE, CYP1A1, Dimethoate, D. polymorpha, Malathion

Destekleyen Kurum

Çalışma herhangi bir kurum kuruluş arafından maddi olarak desteklenmemiştir.

Determination of the Combined Effect of Dimethoate and Malathion Pesticides on Dreissena polymorpha (Pallas, 1771) Using Cytochrome P450 and Acetylcholinesterase

Öz

In this study, cytochrome P450 (CYP1A1) and Acetylcholinesterase (AchE) activities were measured to reveal the toxicity of organophosphorus dimethoate and malathion active ingredient insecticide mixtures in Dreissena polymorpha. The lethal concentration (LC50) of dimethoate and malathion was calculated as 40.82±2.54 mg/L after 96 hours of exposure. D. polymorpha was exposed to three sublethal concentrations of dimethoate and malathion (ratio of 1/16, 1/8 and 1/4 to LC50 value) for 24 hours and 96 hours. CYP1A1 and AChE levels were measured in a microplate reader using the commercial kit. AChE activities were observed to decrease in all exposure groups after 96 hours compared to the control group. When the exposure times were compared, it was determined that there was no statistically significant change in enzyme activities at the end of the 96th hour. There was no statistically significant change in CYP1A1 levels at the end of 24 hours in all groups, but a decrease was observed after 96 hours compared to the control group (p<0.05). It has been determined that the toxic response of the combination of dimethoate and malathion can vary depending on their concentration. In conclusion, it was revealed that dimethoate and malathion mixtures inhibit AChE and CYP1A1 activities in D. polymorpha and these enzymes can be used as an effective biomarker.

Anahtar Kelimeler

AChE, CYP1A1, Dimethoate, D. polymorpha, Malathion

Kaynakça

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