Tissue-Specific Ameliorative Effect of Resveratrol on Oxidative Stress Parameters and Blood Lipid Profile of Mice Exposed to Cadmium

Derya Kocamaz; Oğuz Merhan; Dinçer Erdağ; Hale Ergin Eğritağ; Kadir Bozukluhan

doi:10.35229/jaes.1178638

TR EN

Kadmiyuma Maruz Bırakılan Farelerin Oksidatif Stres Parametreleri ve Kan Lipid Profili üzerinde Resveratrolun Dokuya Özgü İyileştirici Etkisi

Öz

Kadmiyum serbest radikallerin fazla üretimi ve antioksidan savunma sisteminin baskılanması yoluyla oksidatif hasara neden olabilir. Antioksidan özelliklere sahip bir nutrasötik olarak bilinen resveratrolün ağır metallerin toksisitesine karşı koruyucu etkileri olduğu kabul edilmektedir. Çalışmamızda resveratrolün sub-akut kadmiyum maruziyetinin toksik etkilerini bastırıp bastıramayacağı araştırılmıştır. Deney hayvanları 1) Kontrol, 2) Aracı kontrol, 3) Kadmiyum (Cd), 4) Resveratrol (Res) 5) Kadmiyum + Resveratrol (Cd+Res) olacak şekilde gruplara ayrılmıştır. Kadmiyuma maruz kalan farelerin testis ve akciğer dokularında artan lipid peroksidasyonu ve total sialik asit düzeylerinin yanı sıra azalan PON1 düzeyi ve GSH miktarı belirlenmiştir. İlginç bir şekilde, Res ve Cd+Res uygulanan farelerin akciğer dokularında artan lipid peroksidasyonu ve NO düzeyi belirlenmesine rağmen, testis dokularında benzer etkiler belirlenmemiştir. Ayrıca, Cd ve Cd+Res uygulanan farelerde kontrol grubu ile karşılaştırıldığında kanda trigliserit, total kolesterol, çok düşük yoğunluklu lipoproteinler ve düşük yoğunluklu lipoproteinlerin artmasının yanı sıra yüksek yoğunluklu lipoproteinler azaldığı saptanmıştır. Sonuçlarımız kadmiyumun lipid peroksidasyonunu ve total siyalik asit miktarını artırarak, antioksidan düzeylerini azaltarak ve aynı zamanda lipid profilini de etkileyerek dokularda oksidatif hasarı indüklediğini göstermektedir. Ayrıca, bulgularımız resveratrolün sadece testis dokusunda kadmiyum maruziyetine karşı “iyileştirici” bir faktör olarak hareket edebileceğini göstermektedir. Bu nedenle, resveratrolün iyileştirici etkisinin organizmaların ağır metallere maruz kalma dozuna, maruz kalma süresine ve maruz kalınan dokuya bağlı olarak değişiklik gösterebileceğini söyleyebiliriz.

Anahtar Kelimeler

Antioksidan savunma sistemi , Ağır Metaller , Lipid Profili , Doğal polifenoller , Oksidatif Stres

Tissue-Specific Ameliorative Effect of Resveratrol on Oxidative Stress Parameters and Blood Lipid Profile of Mice Exposed to Cadmium

Abstract

Cadmium can cause oxidative damage in organisms through overproduction of free radicals and suppression of the antioxidant defense system. Resveratrol is known as a nutraceutical with antioxidant properties accepted to have protective effects to the toxicity of heavy metals. In this study, we investigated if resveratrol could overwhelm the toxic effect of sub-acute cadmium exposure. Animals were divided to the following groups: 1) Control, 2) Vehicle control, 3) Cadmium (Cd), 4) Resveratrol (Res), 5) Exposure to both Cd and Res (Cd+Res). Increased lipid peroxidation and total sialic acids were determined in testis and lung tissues of mice exposed to Cd with the decreased in paraoxonase (PON1) level and GSH amount. Interestingly, we also found an increased lipid peroxidation and NO levels in lung tissue of mice exposed to Res and Cd + Res, but not in testis tissue. Moreover, increased triglycerides, total cholesterol, very low-density lipoproteins, and low-density lipoproteins as well as reduced high-density lipoproteins were found in mice exposed to Cd and Cd + Res compared to controls. Our results revealed that cadmium induces oxidative damage in tissues, via increased lipid peroxidation, total sialic acids and decreased antioxidant levels as well as disturbs lipid profile. Moreover, our findings indicate that resveratrol may act as “ameliorative” factor against the cadmium exposure in only testis tissue. Thus, we may suggest that ameliorative effect of resveratrol may vary depending on the exposure dose, exposure duration and exposed tissues of animals to the heavy metals.

Keywords

Antioxidant defense system , Heavy Metals , Lipid Profile , Natural polyphenols , Oxidative Stress

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Derya Kocamaz ^*
0000-0002-0705-4672
Türkiye

Oğuz Merhan
0000-0002-3399-0667
Türkiye

Dinçer Erdağ
0000-0001-7137-4403
Türkiye

Hale Ergin Eğritağ
0000-0003-4240-4698
Türkiye

Kadir Bozukluhan
0000-0003-4929-5156
Türkiye

Yayımlanma Tarihi

31 Aralık 2022

Gönderilme Tarihi

22 Eylül 2022

Kabul Tarihi

5 Aralık 2022

Yayımlandığı Sayı

Yıl 2022 Cilt: 7 Sayı: 4

DOI

https://doi.org/10.35229/jaes.1178638

IZ

https://izlik.org/JA93SC49BY

APA

Kocamaz, D., Merhan, O., Erdağ, D., Ergin Eğritağ, H., & Bozukluhan, K. (2022). Tissue-Specific Ameliorative Effect of Resveratrol on Oxidative Stress Parameters and Blood Lipid Profile of Mice Exposed to Cadmium. Journal of Anatolian Environmental and Animal Sciences, 7(4), 493-501. https://doi.org/10.35229/jaes.1178638

Cited By

Protective effects of resveratrol on the expression of catalase, glutathione peroxidase, and superoxide dismutase genes in the ovary and their activity in the serum of rats exposed to lead acetate: An experimental study

International Journal of Reproductive BioMedicine (IJRM)

https://doi.org/10.18502/ijrm.v22i11.17821

Melatonin may protect the gastrointestinal system from cadmium chloride toxicity during pregnancy

Toxicology Research

https://doi.org/10.1093/toxres/tfaf003

JAES, 2016- 11.yıl

Kadmiyuma Maruz Bırakılan Farelerin Oksidatif Stres Parametreleri ve Kan Lipid Profili üzerinde Resveratrolun Dokuya Özgü İyileştirici Etkisi

Öz

Anahtar Kelimeler

Tissue-Specific Ameliorative Effect of Resveratrol on Oxidative Stress Parameters and Blood Lipid Profile of Mice Exposed to Cadmium

Abstract

Keywords

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

Protective effects of resveratrol on the expression of catalase, glutathione peroxidase, and superoxide dismutase genes in the ovary and their activity in the serum of rats exposed to lead acetate: An experimental study

Melatonin may protect the gastrointestinal system from cadmium chloride toxicity during pregnancy