Kültür Kökenli Karaca Mersin Balığı (Acipenser gueldenstaedtii)’nın Hematolojik Parametrelerinin Referans Değerlerinin Belirlenmesi

Yıl 2023, , 88 - 94, 31.03.2023

Kübra Ak , Akif Er , Mert Minaz

https://doi.org/10.35229/jaes.1231567

Öz

Karadeniz havzasının doğal türlerinden olan Karaca mersin balığı (Acipenser gueldenstaedtii), ekolojik öneminin yanı sıra, et kalitesi ve havyarı ile ekonomik değeri de yüksek bir balık türüdür.
Bu çalışmanın amacı, yetiştiricilik ortamında sağlıklı Karaca mersin balığı yavru ve yetişkin bireylerinin hematolojik parametrelerinin referans aralıklarını belirlemektir. Çalışmada, otomatik tam kan sayım cihazı kullanılarak, hematolojik parametreler ölçülmüştür. Tam kan sayımı için lökosit (WBC), lenfosit (LYM), granülasit (Gran), monosit (MID), hematokrit (HCT), hemoglobin (HGB), eritrosit (RBC), ortalama eritrosit hacmi (MCV), ortalama eritrosit hemoglobini (MCH) ve ortalama eritrosit hemoglobin konsantrasyonu (MCHC) değerlerinin referans aralıkları belirlenmiştir.
Çalışmada elde edilen veriler incelendiğinde, yaşa bağlı olarak Karaca mersin balığının ortalama WBC, LYM, MID, GRAN, MCV, MCH ve MCHC değerleri anlamlı bir farklılık göstermiştir. Bununla birlikte RBC, HGB ve HCT değerleri arasında anlamlı bir farklılık tespit edilmemiştir.

Anahtar Kelimeler

Acipenser gueldenstaedtii, akuakültür, mersin balığı, kan parametreleri, otomatik kan sayımı, Acipenser gueldenstaedtii, aquaculture, sturgeon, blood parameters, automatic blood analyser.

Destekleyen Kurum

çalışma herhangi bir fon tarafından desteklenmemiştir.

Determination of Reference Intervals for Hematological Parameters in Cultured Diamond Sturgeon (Acipenser gueldenstaedtii)

Öz

Diamond sturgeon (Acipenser gueldenstaedtii), one of the native species in the Black Sea basin, is economically an important species with its meat quality and caviar as well as ecological importance.
The aim of this study is to determine the reference ranges of hematological parameters in healthy Danube sturgeon juvenile and adult individuals in aquaculture environment. In the study, hematological parameters were measured by an automatic blood count device. Reference ranges were determined for leukocyte (WBC), lymphocyte (LYM), granulocytes (Gran), monocytes (MID), hematocrit (HCT), hemoglobin (HGB), red blood cell (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) values as hematological indicators.
According to the data obtained in the study, mean WBC, LYM, MID, GRAN, MCV, MCH and MCHC values in the sturgeon showed a significant difference depending on age. However, no significant difference was detected between RBC, HGB and HCT values

Anahtar Kelimeler

aquaculture, sturgeon, blood parameters, automatic blood analyser.

Kaynakça

• Adel, M., Safari, R., Yeganeh, S., Satheesh Kumar, P. & Safaie, P. (2017). Hematological and biochemical profile of pike breeders (Esox lucius Linneaus, 1758) from the Anzali Wetland, Caspian Sea. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 87(4), 1271-1276.
• Ahmed, I., Reshi, Q.M. & Fazio, F. (2020). The influence of the endogenous and exogenous factors on hematological parameters in different fish species: a review. Aquaculture International, 28(3), 869-899.
• Ak, K., Kurtoğlu, İ. Z., Serezli, R., KAYIŞ, Ş. & Yandı, İ. (2019). Introduce the Siberian sturgeon (Acipenser baerii) to Turkish aquaculture industry: Duoculture possibility with rainbow trout. Ege Journal of Fisheries and Aquatic Sciences, 36(3), 211-217.
• Ak, K., Minaz, M., Er, A. & Aslankoç, R. (2022). The using potential of a new natural anesthetic agent on rainbow trout (Oncorhynchus mykiss): Chamomile oil (Matricaria chamomilla). Aquaculture, 561, 738-742.
• Altınçekiç, Ş.Ö. & Koyuncu, M. (2010). Nakil koşullarının hayvan refahı üzerine etkileri. Hayvansal Üretim, 51, 48-56.
• Alyakrinskyaya, I.O. & Dolgova, S.N. (1984). Hematological features of young sturgeons. Vopr Ikhtiol, 4, 135-139.
• Baghizadeh, E., Sadeghpour, A., Khara, H. & Nezami, S.A. (2014). Effect of age on some blood hücre and biochemical factors of common carp Cyprinus carpio Linnaeus 1758. Journal of Aquatic Animals & Fisheries 4(16), 17-24.
• Bahmani, M., Kazemi, R. & Donskaya, P. (2011). A comparative study of some hematological features in young reared sturgeons (Acipenser persicus and Huso huso). Fish Physiol Biochem. 24, 135-140.
• Bemis, W.E., Birstein, V.J. & Waldman, J.R. (1997). Sturgeon biodiversity and conservation: an introduction. Sturgeon Biodiversity and Conservation, 454, 13-14. Springer, Dordrecht.
• Beridze, T., Boscari, E., Scheele, F., Edisherashvili, T., Anderson, C. & Congiu, L. (2022). Interspecific hybridization in natural sturgeon populations of the Eastern Black Sea: the consequence of drastic population decline? Conservation Genetics, 23(1), 211-216.
• Bronzi, P., Rosenthal, H. & Gessner, J. (2011). Global sturgeon aquaculture production: an overview. Journal of Applied Ichthyology, 27(2), 169-175.
• Cassle, S.E., Yanong, R.P., Pouder, D.B., Rodriguez, C., Mylniczenko, N., Thompson, P. M. & Stacy, N.I. (2021). Reference intervals for blood analytes of adult aquarium‐housed Russian sturgeon Acipenser gueldenstaedtii. Journal of Aquatic Animal Health, 33(1), 33-43.
• Clauss, T.M., Dove, A.D. & Arnold, J.E. (2008). Hematologic disorders of fish. Veterinary Clinics of North America: Exotic Animal Practice, 11(3), 445-462.
• Dönmez, E.A., Kolay, M., Özkan, F. & Koyuncu, C.E. (2006). FMC ve malaşit yeşili sağaltım dozlarının (Oreochromis niloticus L., 1758) bazı kan parametrelerinde meydana getirdiği değişimler. Journal of Fisheries and Aquatic Sciences, 23(1/1), 61-64.
• Duman, S. (2020). Determination of reference values of some hematological and immunological parameters in healthy Russian sturgeon (Acipenser gueldenstaedtii). Journal of Anatolian Environmental and Animal Sciences, 5(2), 212- 217.
• Esmaeili, M. (2021). Blood performance: A new formula for fish growth and health. Biology, 10(12), 1236.
• Fallah, F.J., Khara, H., Roohi, J.D. & Boorani, M.S. (2014). Hematological parameters of pike Esox lucius in relation to different ages and seasons. Comparative Clinical Pathology, 23(4), 949-953.
• Fazio, F. (2019). Fish hematology analysis as an important tool of aquaculture: a review. Aquaculture, 500, 237-242.
• Fazio, F., Marafioti, S., Arfuso, F., Piccione, G. & Faggio, C. (2013). Influence of different salinity on haematological and biochemical parameters of the widely cultured mullet, Mugil cephalus. Marine and Freshwater Behaviour and Physiology, 46(4), 211-218.
• Ghiasi, S., Falahatkar, B., Dabrowski, K., Abasalizadeh, A. & Arslan, M. (2014). Effect of thiamine injection on growth performance, hematology and germinal vesicle migration in sterlet sturgeon Acipenser ruthenus L. Aquaculture International, 22(5), 1563-1576. DOI: 10.1007/s10499-014-9765-77.
• Hrubec, T.C. & Smith, S.A. (2000). Hematology of fish. In: Feldman BF, Zinkl JG, Jain NC (eds) Schalm’s Veterinary Hematology, 5th. Philadelphia, 1120- 1125.
• Hrubec, T.C., Robertson, J.L. & Smith, S.A. (1997). Effects of temperature on hematologic and serum biochemical profiles of hybrid striped bass (Morone chrysops x Morone saxatilis). Am. J. Vet. Res., 58,126-130.
• Hurvitz, A., Jackson, K., Degani, G. & Levavi-Sivan, B. (2007). Use of endoscopy for gender and ovarian stage determinations in Russian sturgeon (Acipenser gueldenstaedtii) grown in aquaculture. Aquaculture, 270(1-4), 158-166.
• IUCN. (2020). The IUCN Red List of Threatened Species. From: https://www.iucnredlist.org/
• Jamalzadeh, H.R. & Ghomi, M.R. (2009). Hematological parameters of Caspian salmon Salmo trutta caspius associated with age and season. Marine and Freshwater Behaviour and Physiology, 42(1), 81-87.
• Karimi, S.H., Kochinian, P. & Salati, A.P. (2013). The effect of sexuality on some haematological parameters of the yellowfin seabream, Acanthopagrus latus in Persian Gulf. Iranian Journal of Veterinary Research, 14(1), 65-68.
• Keleştemur, G.T. & Özdemir, Y. (2010). Nakil işleminin gökkuşağı alabalıkları (Oncorhynchus mykiss, W. 1792)’nın bazı kan parametre değerleri üzerine etkileri. Süleyman Demirel Üniversitesi, Fen Bilimleri Enstitüsü Dergisi, 14(3), 213-217.
• Knowles, S., Hrubec, T.C., Smith, S.A. & Bakal, R.S. (2006). Hematology and plasma chemistry reference intervals for cultured shortnose sturgeon (Acipenser brevirostrum). Veterinary Clinical Pathology, 35(4), 434-440.
• Matsche, M.A., Arnold, J., Jenkins, E., Townsend, H. & Rosemary, K. (2014). Determination of hematology and plasma chemistry reference intervals for 3 populations of captive Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus). Veterinary Clinical Pathology, 43(3), 387-396. DOI: 10.1111/vcp.12174.
• Memiş, D. (2007). Sturgeon aquaculture. A. Candan, S. Karataş, Kűçűktas & L. Okumuş, eds. Marine aquaculture in Turkey. Istanbul, Turkish Marine Research Foundation, 49-59.
• Minaz, M., Er, A., Ak, K., Nane, İ. D., İpek, Z.Z., Kurtoğlu, İ. Z. & Kayış, Ş. (2022). Short-term Exposure to Bisphenol A (BPA) as a Plastic Precursor: Hematological and Behavioral Effects on Oncorhynchus mykiss and Vimba vimba. Water, Air, & Soil Pollution, 233(4), 1-12.
• Moeller, J. & Robert, B. (2001). California animal healt and food safety: Diseases of fish. California Press, 43, 34.
• Mylonas C.C., Cardinaletti G., Sigelaki I. & Polzonetti- Magni A. (2005). Comparative Efficacy of Clove Oil and 2-phenoxyethanol as Anesthetics in the Aquaculture of European Sea Bass (Dicentrarchus labrax) and Gilthead Sea Bream (Sparus aurata) at Different Temperatures. Aquaculture, 246(1-4): 467-481.
• Noyan, A. (1993). Yaşam ve Hekimlikte Fizyoloji. 8. Baskı. Meteksan Yayınevi, Ankara.
• Ranzani-Paiva, M.J.T., Rodrigues, E.L., Veiga, M.L., Eiras, A.C. & Campos, B.E.S. (2003). Differential leucokocyte counts in ''dourado'', Salminus maxillosus Valenciennes, 1840, from the Mogi-Guaçu River, Pirassununga, sp. Brazilian Journal of Biology, 63, 517-525.
• Rehulka, J., Minařík, B. & Řehulková, E. (2004). Red blood hücre indices of rainbow trout Oncorhynchus mykiss (Walbaum) in aquaculture. Aquaculture Research, 35(6), 529-546.
• Romano, N., Scapigliati, G. & Abelli, L. (2017). Water oxygen content affects distribution of T and B lymphocytes in lymphoid tissues of farmed sea bass (Dicentrarchus labrax). Fishes, 2(3), 16.
• Ruban, G.I. (2019). Adaptive ecological and morphological features of the Siberian sturgeon (Acipenser baerii Brandt). Inland Water Biology, 12(2), 210-216.
• Sadati, M.A.Y., Pourkazemi, M., Shakurian, M., Hasani, M.H.S., Pourali, H.R., Pourasaadi, M. & Yousefi, A. (2011). Effects of daily temperature fluctuations on growth and hematology of juvenile Acipenser baerii. Journal of Applied Ichthyology, 27(2), 591-594.
• Sebastiao, F.A., Nomura, D., Sakabe, R. & Pilarski, F. (2011). Hematology and productive performance of Nile tilapia (Oreochromis niloticus) naturally infected with Flavobacterium columnare. Brazilian Journal of Microbiology, 42, 282-289.
• Speckner, W., Schindler, J.F. & Albers, C. (1989). Age- dependent changes in volume and haemoglobin content of erythrocytes in the carp (Cyprinus carpio L.). Journal of Experimental Biology, 141(1), 133-149. DOI: 10.1007/s00580-014- 1983-x.
• Ustaoğlu Tırıl, S. & Memiş, D. (2018). An overview of the factors affecting the migration of sturgeons in Yeşilırmak. Aquatic Sciences and Engineering, 33(4), 138-144. DOI:10.26650/ASE20180002.
• Val, A.L., De Menezes, G.C. & Wood C.M. (1998). Red Blood Cell Adrenergic Responses in Amazonian Teleost. J. Fish Biol., 52: 83-93.
• Vázquez, G.R. & Guerrero, G.A. (2007). Characterization of blood hücres and hematological parameters in Cichlasoma dimerus (Teleostei, Perciformes). Tissue and Hücre, 39(3), 151-160.
• Vinodhini, R. & Narayanan, M. (2009). The impact of toxic heavy metals on the hematological parameters in common carp (Cyprinus carpio L.). Journal of Environmental Health Science & Engineering, 6(1), 23-28.
• Witeska, M. (2015). Anemia in teleost fishes. Bull. Eur. Assoc. Fish. Pathol., 35(4).
• Witeska, M., Kondera, E., Ługowska, K. & Bojarski, B. (2022). Hematological methods in fish–Not only for beginners. Aquaculture, 547, 737-498.
• Xu, Z., Cao, J., Qin, X., Qiu, W., Mei, J. & Xie, J. (2021). Toxic effects on bioaccumulation, hematological parameters, oxidative stress, immune responses and tissue structure in fish exposed to ammonia nitrogen: a review. Animals, 11, 3304.
• Yarmohammadi, M., Pourkazemi, M., Kazemi, R., Pourdehghani, M., Hassanzadeh Saber, M. & Azizzadeh, L. (2015). Effects of starvation and re-feeding on some hematological and plasma biochemical parameters of juvenile Persian sturgeon, Acipenser persicus Borodin, 1897. Caspian Journal of Environmental Sciences, 13(2), 129-140.
• Yousefzadeh, F. & Khara, H. (2015). Changes in blood chemistry and hematological indices of Capoeta capoeta gracilis in relation to age, sex, and geographic location. Comparative Clinical Pathology, 24, 791-795.
• Zakes, Z., Demska-Zakes, K., Szczepkowski, M., Rozynski, M. & Ziomek, E. (2016). Impact of sex and diet on hematological and blood plasma biochemical profiles and liver histology of pike perch (Sander lucioperca (L.)). Arch Pol Fisheries 24,61-68. DOI: 10.1515/aopf-2016- 0007.