Vibrio hepatarius septicemia with subacute enteritis in common guitarfish, Rhinobatos rhinobatos

Yıl 2023, , 211 - 217, 30.06.2023

Kemal Bağcı , Neslihan Köstebekci , Merve Taşgın , Ebrucan Bulut , Kübra Balcı , Ece Yurddas , Nihed Ajmi , Burak Ozdemir , Fatih Hira Aytekin , Özkan Yavaş , Muhammed Duman

https://doi.org/10.35229/jaes.1281938

Öz

Common guitarfish (Rhinobatos rhinobatos) are an endangered species and are placed on the red list of “Critically Endangered, CR” by the International Union for Conservation of Nature. Guitarfish are kept in captivity and are protected in public aquaria globally, but there is limited information about the diseases for this species. In this research, mortalities (14%) observed in common guitarfish in a public aquarium are reported. Severe hemorrhagic lesions on the ventral body side and reduced feed intake were observed in the affected animals. Two guitarfish were referred for necropsy, microbiological and histopathological examination. Macroscopic findings included severe ecchymotic hemorrhages in the ventral body, bloating of the gastrointestinal tract due to invasive mass, and hyperemia of the colon vessels and obstruction of the colon lumen. Histopathological examination revealed degenerations and necrosis in the liver, desquamation of intestinal villi, severe mononuclear cell infiltration and fibrosis in distal intestine, severe bacterial aggregates in different organs, pancreatitis, vasculitis, and filamentous bacteria-like structures. Vibrio hepatarius was isolated and identified with multilocus sequence analysis (MLSA); multilocus sequence typing (MLST) revealed that the agent belonged to a novel sequence type, 247. After the first description of V. hepatarius in wild healthy adults of Whiteleg shrimp (Litopenaeus vannamei) from Ecuador, this is the first report of deaths due to V. hepatarius observed in common guitarfish kept in captivity in a public aquarium.

Anahtar Kelimeler

Vibrio hepatarius, common guitarfish, ornamental fish, aquarium fish diseases

Destekleyen Kurum

TUBİTAK

Proje Numarası

1919B012004874

Teşekkür

The study was supported from TUBITAK 2209-A project with project number: 1919B012004874. We are grateful to Assoc. Prof. Dr. Taci Cangul for scientific contributions.

Gitar balıklarında (Rhinobatos rhinobatos) Vibrio hepatarius kaynaklı subakut enteritik septisemi

Öz

Gitar balığı (Rhinobatos rhinobatos) nesli tükenmekte olan bir balık türdür ve Uluslararası Doğayı Koruma Birliği tarafından "Kritik Tehlike Altında, CR" kırmızı listesine alınmıştır. Gitar balığı çoğunlukla akvaryumlarda tutulur ve küresel olarak halka açık akvaryumlarda korunmaya çalışır, ancak bu türün hastalıkları hakkında sınırlı bilgi bulunmaktadır. Bu araştırmada halka açık bir akvaryumda gitar balıklarında görülen mortalitelere neden olan enfeksiyon (%14) araştırılmıştır. Etkilenen hayvanlarda vücudun ventral tarafında önemli hemorajik lezyonlar ve yem alımında azalma gözlenmiştir. İki gitar balığı otopsi, mikrobiyolojik ve histopatolojik inceleme için laboratuvara soğuk zincir altında gönderilmiştir. Makroskobik bulgular ventral bölgede ekimotik kanamalar, invaziv kitle nedeniyle gastrointestinal sistemde şişkinlik ve kolon damarlarında hiperemi ve kolon lümeninde tıkanıklık tespit edilmiştir. Histopatolojik incelemede karaciğerde dejenerasyon ve nekroz, barsak villuslarında deskuamasyon, barsak distalinde şiddetli mononükleer hücre infiltrasyonu ve fibrozis, farklı organlarda ciddi bakteriyel kümelenmeler, pankreatit, vaskülit ve filamentli bakteri benzeri yapılar belirlenmiştir. Vibrio hepatarius etken izolasyonu yapılmış ve multilocus sekans analizi (MLSA) ile karakterize edilmiştir. Multilocus sekans tiplemesi (MLST), etkenin yeni bir sekans tipi olan ST 247'ye ait olduğunu ortaya koymuştur. Ekvador'dan Whiteleg karidesinin (Litopenaeus vannamei) doğal sağlıklı yetişkinlerinde V. hepatarius'un ilk tanımından sonra, bu etkene bağlı ölümler gitar balıklarında ilk defa rapor edilmektedir.

Anahtar Kelimeler

Vibrio hepatarius, gitar balığı, akvaryum balığı, akvaryum balığı hastalıkları

Proje Numarası

1919B012004874

Kaynakça

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