Immunity-Related Enzyme Gene Interactions in Archocentrus centrarchus Infected with Lactococcus garvieae
Abstract
Unsuitable environmental conditions and low host resistance in the aquatic environment are the most important factors in fish diseases. Bacterial diseases in fish can be treated with different preparations. The aim of this study is to determine the disease symptoms, expression levels of TNF and IL genes and some biochemical parameter changes in Archocentrus centrarchus by using herbal extract for the treatment of the disease caused by Lactococcus garvieae bacteria. Gene expression was performed by Real-Time PCR and enzyme activities were determined by spectrophotometric analysis method. It was observed that the expression level of TNF gene was 2.5 times more effective in the antibiotic (oxytetracycline) group and 14 times more effectively in the extract application. It was found that the antibiotic group had a close expression level compared to the control at the IL gene expression level, while the extract group expressed 5.5 times more. Glutathione s-transferase activity in the extract group increased significantly compared to the control and antibiotic groups. Superoxide dismutase activity was significantly higher in the extract group compared to the antibiotic and bacterial groups. It can be said that oxidative stress caused by bacterial infection is tried to be eliminated more in the extract group. It was determined that the andiz root extract which at the patent stage, which was used for the first time in the treatment of A. centrarchus fish infected with L. garvieae, one of the most important bacterial agents in the field of aquaculture, is applicable.
Keywords
Cichlid fish , fish diseases , gene expression , IL , TNF
Kaynakça
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