The Anti-Proliferative Effects of Ficus carica Latex on Cancer and Normal Cells

Ferhunde Aysin; Nihal Şimşek Özek; Nurullah Acet; Kubra Koc

doi:10.35229/jaes.1412816

TR EN

Ficus carica Sütünün Kanser ve Normal Hücre Üzerindeki Anti-proliferatif Etkileri

Öz

İncir sütü, olgunlaşmamış incir meyve veya yaprak saplarının içerisinde bulunan beyaz renkli bir sıvıdır. İncir sütü, halk hekimliğinde çeşitli amaçlar için kullanılmaktadır ve modern çalışmalarda üstün farmakolojik özelliklere sahip olduğu kanıtlanmıştır. Ayrıca incir sütünün kozmetik açıdan endüstriyel değeri gitgide artmaktadır. Mevcut çalışmada, Ficus carica (F. carica) türünden elde edilen incir sütünün akciğer kanseri (A549), meme kanseri (MCF-7), üçlü negatif meme kanseri (MDA-MB-231) ve tümörojenik olmayan akciğer (MRC-5) hücre hatları üzerindeki sitotoksik etkisi MTT analiziyle değerlendirildi. Tüm hücre hatları, incir sütünün 1/1500-1/12 arası değişen konsantrasyonlarıyla 48 saat boyunca muamele edildi. İncir sütü, kanserli hücrelerde konsantrasyona bağlı bir şekilde hücre ölüm oranını arttırdı ve yüksek dozlarında hücreler üzerinde daha ciddi bir sitotoksisik etkiye neden oldu. Sağlıklı hücrelerde ise kanser hücrelerine göre daha az toksik etki gösterdi. İncir sütünün IC50 değeri, A549 hücrelerinde 1/26, MCF-7 hücrelerinde 1/40, MDA-MB-231 hücrelerinde 1/45 ve MRC-5 hücrelerinde ise 1/7 olarak belirlendi. Bulgularımız, incir sütünün kanser hücrelerine karşı seçici sitotoksisiteye sahip olduğunu ve gelecekte kanserle ilgili ilaç araştırmalarındaki ilerlemelerde faydalı olabileceğini göstermektedir. İncir sütünün ve muhtevasındaki biyoaktif bileşiklerinin fonksiyonel özellikleri açısından kanser tedavilerinde potansiyel kullanımına dair bilimsel araştırmalara daha fazla ihtiyaç vardır.

Anahtar Kelimeler

İncir sütü , MTT , akciğer kanseri , meme kanseri , akciğer fibroblastı

The Anti-Proliferative Effects of Ficus carica Latex on Cancer and Normal Cells

Abstract

The stems of unripe fig fruits or leaves contain a white liquid called fig latex. Fig latex contains compounds that are effective against cancer cells. It is used for various purposes in folk medicine and has been proven to have superior pharmacological properties in modern studies. The industrial value of latex from a cosmetic perspective is increasing as well. In the current study, the cytotoxic effect of fig latex obtained from Ficus carica was evaluated by MTT analysis on lung cancer (A549), breast cancer (MCF-7), triple-negative breast cancer (MDA-MB-231), and non-tumorogenic lung (MRC-5) cell lines. All cell lines were treated with various dilutions of fig latex ranging from 1/1500 to 1/12 for 48 hours. In cancer cells, it induced cell death in a concentration-dependent manner, and high doses caused a more serious cytotoxic effect. On the other hand, it showed less cytotoxicity on healthy cells compared to cancer cells. Fig latex IC50 values were found to be 1/26, 1/40, 1/45, and 1/7 for A549, MCF-7, MDA-MB-231, and MRC-5 cells, respectively. Based on our findings, fig latex showed selective cytotoxicity against cancer cells, making it a possible target for future cancer drug development. However, further research is needed to fully understand the potential use of fig latex and its bioactive compounds in cancer treatment in terms of their functional properties.

Keywords

Fig latex , MTT , lung cancer , breast cancer , lung fibroblast

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Hayvan Fizyolojisi - Hücre

Bölüm

Araştırma Makalesi

Yazarlar

Ferhunde Aysin
0000-0003-1971-8031
Türkiye

Nihal Şimşek Özek
0000-0002-7326-5670
Türkiye

Nurullah Acet Bu kişi benim
0000-0002-8382-7200
Türkiye

Kubra Koc ^*
0000-0001-6208-165X
Türkiye

Erken Görünüm Tarihi

14 Haziran 2024

Yayımlanma Tarihi

30 Haziran 2024

Gönderilme Tarihi

3 Ocak 2024

Kabul Tarihi

4 Nisan 2024

Yayımlandığı Sayı

Yıl 2024 Cilt: 9 Sayı: 2

DOI

https://doi.org/10.35229/jaes.1412816

IZ

https://izlik.org/JA57NC24SE

APA

Aysin, F., Şimşek Özek, N., Acet, N., & Koc, K. (2024). The Anti-Proliferative Effects of Ficus carica Latex on Cancer and Normal Cells. Journal of Anatolian Environmental and Animal Sciences, 9(2), 145-151. https://doi.org/10.35229/jaes.1412816