The Anti-Proliferative Effects of Ficus carica Latex on Cancer and Normal Cells

Yıl 2024, , 145 - 151, 30.06.2024

Ferhunde Aysin , Nihal Şimşek Özek , Nurullah Acet Kubra Koc

https://doi.org/10.35229/jaes.1412816

Öz

The stems of unripe fig fruits or leaves contain a white liquid called fig latex. Fig latex contains compounds that are effective against cancer cells. It is used for various purposes in folk medicine and has been proven to have superior pharmacological properties in modern studies. The industrial value of latex from a cosmetic perspective is increasing as well. In the current study, the cytotoxic effect of fig latex obtained from Ficus carica was evaluated by MTT analysis on lung cancer (A549), breast cancer (MCF-7), triple-negative breast cancer (MDA-MB-231), and non-tumorogenic lung (MRC-5) cell lines. All cell lines were treated with various dilutions of fig latex ranging from 1/1500 to 1/12 for 48 hours. In cancer cells, it induced cell death in a concentration-dependent manner, and high doses caused a more serious cytotoxic effect. On the other hand, it showed less cytotoxicity on healthy cells compared to cancer cells. Fig latex IC50 values were found to be 1/26, 1/40, 1/45, and 1/7 for A549, MCF-7, MDA-MB-231, and MRC-5 cells, respectively. Based on our findings, fig latex showed selective cytotoxicity against cancer cells, making it a possible target for future cancer drug development. However, further research is needed to fully understand the potential use of fig latex and its bioactive compounds in cancer treatment in terms of their functional properties.

Anahtar Kelimeler

Fig latex, MTT, lung cancer, breast cancer, lung fibroblast

Ficus carica Sütünün Kanser ve Normal Hücre Üzerindeki Anti-proliferatif Etkileri

Öz

İncir sütü, olgunlaşmamış incir meyve veya yaprak saplarının içerisinde bulunan beyaz renkli bir sıvıdır. İncir sütü, halk hekimliğinde çeşitli amaçlar için kullanılmaktadır ve modern çalışmalarda üstün farmakolojik özelliklere sahip olduğu kanıtlanmıştır. Ayrıca incir sütünün kozmetik açıdan endüstriyel değeri gitgide artmaktadır. Mevcut çalışmada, Ficus carica (F. carica) türünden elde edilen incir sütünün akciğer kanseri (A549), meme kanseri (MCF-7), üçlü negatif meme kanseri (MDA-MB-231) ve tümörojenik olmayan akciğer (MRC-5) hücre hatları üzerindeki sitotoksik etkisi MTT analiziyle değerlendirildi. Tüm hücre hatları, incir sütünün 1/1500-1/12 arası değişen konsantrasyonlarıyla 48 saat boyunca muamele edildi. İncir sütü, kanserli hücrelerde konsantrasyona bağlı bir şekilde hücre ölüm oranını arttırdı ve yüksek dozlarında hücreler üzerinde daha ciddi bir sitotoksisik etkiye neden oldu. Sağlıklı hücrelerde ise kanser hücrelerine göre daha az toksik etki gösterdi. İncir sütünün IC50 değeri, A549 hücrelerinde 1/26, MCF-7 hücrelerinde 1/40, MDA-MB-231 hücrelerinde 1/45 ve MRC-5 hücrelerinde ise 1/7 olarak belirlendi. Bulgularımız, incir sütünün kanser hücrelerine karşı seçici sitotoksisiteye sahip olduğunu ve gelecekte kanserle ilgili ilaç araştırmalarındaki ilerlemelerde faydalı olabileceğini göstermektedir. İncir sütünün ve muhtevasındaki biyoaktif bileşiklerinin fonksiyonel özellikleri açısından kanser tedavilerinde potansiyel kullanımına dair bilimsel araştırmalara daha fazla ihtiyaç vardır.

Anahtar Kelimeler

İncir sütü, MTT, akciğer kanseri, meme kanseri, akciğer fibroblastı

Kaynakça

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