Thiol/disulfide Homeostasis and Some Minerals in Aging

Yıl 2024, , 190 - 195, 30.06.2024

Recai Aci , Gülay Çiftci , Furkan Ümit , Pınar Kar , Mustafa Ermiş , Özüm Çaka

https://doi.org/10.35229/jaes.1436383

Öz

The aim of the study was to investigate how aging affects thiol/disulphide balance and
serum mineral levels in rats of different ages. Twenty-four healthy male Sprague-Dawley rats in
good health were used as study subjects. Group 1 consisted of 1.5-month-old baby rats, followed
by 6-month-old, 12-month-old and 18-month-old groups. Sodium (Na), potassium (K), chloride
(Cl), phosphorus (P), calcium (Ca), iron (Fe) and magnesium (Mg) concentrations in serum were
measured by spectrophotometric method. Natural Thiol (NTL) and Total Thiol (TTL)
concentrations were determined using Rel Assay Diagnostics Equipment. The difference between
Na, Fe and Mg levels was not statistically significant (P>0.05). Compared with group 1, Ca levels
of groups 2 and 3 were shown to be significantly lower. Growth resumed in group 4 (P<0.05). K
levels were lower in groups 2 and 3 compared to group 1. In group 4, it started to increase
(P>0.05). Cl level started to increase with increasing age and this increase was significant between
groups 1 and 4 (P<0.05). While the difference between Group 3 and Group 4 was significant
(P<0.05), the difference between Group 2, which had the highest TTL and NTL levels, and Group
1, which started to decrease with age, was not significant (P>0.05). It has been found that disulfide
levels begin to increase as we age (P>0.05). Mineral amounts and thiol/disulphide balance were
found to change with age.

Anahtar Kelimeler

aging, thiol/disulfide hoöeostasis, mineral

Yaşlanmada tiyol/disülfür Homeostazisi ve Bazı Mineraller

Öz

Araştırmada farklı yaşlardaki sıçanlarda yaşlanmanın tiyol/disülfit dengesi ve serum mineral
düzeylerine etkisinin araştırılması amaçlandı. Çalışma denekleri olarak sağlık durumu iyi 24
sağlıklı erkek Sprague-Dawley sıçanı kullanıldı. Grup 1'de 1,5 aylık yavru sıçanlar yer alırken
bunu 6 aylık, 12 aylık ve 18 aylık gruplar takip etti. Serumdaki sodyum (Na), potasyum (K),
klorür (Cl), fosfor (P), kalsiyum (Ca), demir (Fe) ve magnezyum (Mg) konsantrasyonları
spektrofotometrik yöntem kullanılarak ölçüldü. Doğal Tiol (NTL) ve Toplam Tiyol (TTL)
konsantrasyonları Rel Assay Diagnostics Equipment kullanılarak belirlendi. Farklı yaş
gruplarındaki sıçanların Na, Fe ve Mg seviyeleri arasındaki fark istatistiksel olarak önemli
bulunmadı (P>0.05). Grup 1 ile karşılaştırıldığında Grup 2 ve 3'ün Ca düzeylerinin anlamlı
derecede düşük olduğu görüldü. Grup 4'te artmaya başladı (P<0.05). Grup 2 ve 3'te K
düzeylerinin Grup 1'e göre daha düşük olduğu görüldü. Grup 4'te ise artmaya başladı
(P>0.05).Yaş arttıkça Cl düzeyi artmaya başladı ve Grup 1 ile 4 arasında bu artış anlamlıydı
(P<0.05). Grup 3 ve Grup 4 arasındaki fark anlamlı iken (P<0.05), Grup 2'de TTL ve NTL
düzeyleri en yüksek olan ve yaşlanmayla birlikte düşmeye başlayan Grup 1 arasındaki farkın
anlamlı olmadığı (P>0.05) tespit edildi. Yaş aldıkça disülfit seviyelerinin artmaya başladığı
bulundu (P>0.05). Mineral miktarlarının ve tiyol/disülfür dengesinin yaşla birlikte değiştiği tespit
edildi.

Anahtar Kelimeler

yaşlanma, mineral, tiyol/disülfür homeostazisi

Kaynakça

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