Sentetik Atıksuyun Dreissena polymorpha'da Antioksidan Savunma Sistemine Etkisi

Yıl 2024, , 307 - 313, 30.09.2024

Nuran Cikcikoglu Yildirim , Osman Serdar , Gökhan Önder Ergüven , Gozde Ergul , Numan Yıldırım

https://doi.org/10.35229/jaes.1471110

Öz

Sentetik atık suyun Dreissena polymorpha'da antioksidan savunma sistemi üzerine etkileri değerlendirildi. Bu amaçla ticari kitler kullanılarak dokulardaki SOD, CAT, GPX aktiviteleri ile TBARS ve GSH düzeyleri belirlendi. Sentetik grupta SOD ve CAT aktivitelerinin D. polymorpha'da 24 ve 96 saat boyunca kontrol grubuna göre daha düşük olduğu belirlendi (p<0.05). D. polymorpha örneklerinin hücrelerinin sentetik atık suya maruz bırakılmasından sonra, GSH seviyelerinde azalma ve ardından TBARS seviyelerinde artış gözlendi ve bu durum onların kararsız durumlarını yansıtıyordu. Çalışmadan elde edilen verilere göre izlenen biyobelirteçlerin kentsel atık suyun biyokimyasal mekanizmalarını erken uyarı göstergesi olarak değerlendirmede faydalı ve etkili olduğu sonucuna varılabilir.

Anahtar Kelimeler

Antioksidant, Atıksu, Biyokimyasal mekanizma, Biyomarkır

Etik Beyan

Etik bir durum gerekmemektedir

Effect of Synthetic Wastewater on Antioxidant Defence System in Dreissena polymorpha

Öz

The effects of the synthetic wastewater on antioxidant defence system in Dreissena polymorpha evaluated. For this purpose, SOD, CAT, GPX activities and TBARS and GSH levels in tissues were determined by using commercial kits. The activities of SOD and CAT were in sythtetic group were determined lower than the control group in D. polymorpha during 24 and 96 h (p<0.05). After exposure of the cells of D. polymorpha samples to synthetic wastewater, depletion in GSH levels and subsequent increase in TBARS levels were observed, reflecting their unstable state. According to the data obtained from the study, it can be concluded that the biomarkers monitored are useful and effective in evaluating the biochemical mechanisms of urban wastewater as early warning indicators.

Anahtar Kelimeler

Antioxidant, Biochemical mechanism, Biomarkers

Etik Beyan

There is no need for ethical statement.

Kaynakça

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