Allium pervariensis'in Fenolik Bileşik Profili: LC-HRMS ile Fitokimyasal Analiz ve Potansiyel Uygulamaları

Yıl 2025, Cilt: 10 Sayı: 4, 452 - 456, 31.07.2025

İdris Yolbaş

https://doi.org/10.35229/jaes.1647066

Öz

Allium pervariensis, Türkiye'nin Güneydoğu Anadolu Bölgesi'ne özgü endemik bir bitki türüdür. Fenolik bileşikler bitkilerde önemli ikincil metabolitler olup antioksidan, antikanser ve anti-inflamatuar gibi çeşitli biyolojik aktivitelere sahiptir. Bu çalışma, A. pervariensis’in fenolik bileşik içeriğini belirlemeyi amaçlamaktadır.
Bitki örnekleri LC-HRMS yöntemi kullanılarak analiz edilmiştir. Bu kapsamda toplam 22 fitokimyasal bileşik tespit edilmiş ve konsantrasyonları belirlenmiştir.
LC-HRMS analizi, en yüksek konsantrasyona sahip bileşiklerin apigenin (45705,91 ng/g), vanilik asit (2336,86 ng/g) ve ferulik asit (1644,26 ng/g) olduğunu ortaya koymuştur. Elde edilen veriler, A. pervariensis’in fenolik bileşikler açısından zengin bir kaynak olduğunu göstermektedir.
Bu bulgular, A. pervariensis’in farmasötik, kozmetik ve gıda endüstrilerinde potansiyel kullanım alanlarına sahip olabileceğini düşündürmektedir. Ancak, bitkinin biyolojik aktivitelerinin daha iyi anlaşılabilmesi için ileri çalışmalara ihtiyaç duyulmaktadır.

Anahtar Kelimeler

fitokimyasallar , Allium pervariensis , apigenin , vanilik asit , ferulik asit

Phenolic Compound Profile of Allium pervariensis: Phytochemical Analysis by LC-HRMS and Potential Applications

Öz

Allium pervariensis is an endemic plant species native to the Southeastern Anatolia Region of Turkey. Phenolic compounds are important secondary metabolites in plants and exhibit various biological activities such as antioxidant, anticancer, and anti-inflammatory effects. This study aims to determine the phenolic compound contents of A. pervariensis.Plant samples were analyzed using the LC-HRMS method. In this context, a total of 22 phytochemical compounds were detected, and their concentrations were determined.LC-HRMS analysis revealed that apigenin (45705.91 ng/g), vanillic acid (2336.86 ng/g), and ferulic acid (1644.26 ng/g) were the compounds with the highest concentrations. The data obtained indicate that A. pervariensis is a rich source of phenolic compounds.These findings suggest that A. pervariensis may have potential applications in the pharmaceutical, cosmetic, and food industries. However, further studies are needed to better understand the biological activities of the plant.

Anahtar Kelimeler

phytochemicals , Allium pervariensis , apigenin , vanillic acid , ferulic acid

Etik Beyan

-

Destekleyen Kurum

-

Teşekkür

-

Kaynakça

• Bellikci, E. (2011). Türkiye’de Gastrointestinal Sistem Rahatsızlıklarında Başvurulan Tıbbi Bitkilerin Etki Potansiyelleri. Ege Üniversitesi.
• Choi, H.J., & Oh, B.U. (2011). A partial revision of Allium (Amaryllidaceae) in Korea and north-eastern China. Botanical Journal of the Linnean Society, 167(2), 153- 211.
• del Mar Verde Méndez, C., Forster, M.P., Rodríguez- Delgado, M.Á., Rodríguez-Rodríguez, E.M., & Díaz Romero, C. (2003). Content of free phenolic compounds in bananas from Tenerife (Canary Islands) and Ecuador. European Food Research and Technology, 217(4), 287-290. DOI: 10.1007/s00217- 003-0762-8
• Digrak, M., Alma, M.H., Ilçim, A., & Sen, S. (1999). Antibacterial and antifungal effects of various commercial plant extracts. Pharmaceutical Biology, 37(3), 216-220. DOI: 10.1076/phbi.37.3.216.6307
• Ekşi, G., Özkan, A.M.G., & Koyuncu, M. (2020). Garlic and onions: An eastern tale. Journal of Ethnopharmacology, 253, 112675.
• Emir, A., Emir, C., & Yıldırım, H. (2020). Characterization of phenolic profile by LC‐ESI‐MS/MS and enzyme inhibitory activities of two wild edible garlic: Allium nigrum L. and Allium subhirsutum L. Journal of Food Biochemistry, 44(4). DOI: 10.1111/jfbc.13165
• Eyupoglu, O.E. (2019). Antioxidant activities, phenolic contents and electronic nose analysis of black garlic. International Journal of Secondary Metabolite, 6(2), 154-161.
• Firat, M., Koyuncu, M., & Ekşi, G. (2018). Allium pervariensis, sect. Allium (Amaryllidaceae), a new species from Siirt Turkey. Plant Biosystems - An International Journal Dealing with All Aspects of Plant Biology, 152(3), 305- 310. DOI: 10.1080/11263504.2016.1271051
• Ganesan, K., & Xu, B. (2017). A critical review on polyphenols and health benefits of black soybeans. Nutrients, 9(5), 455.
• Imran, M., Aslam Gondal, T., Atif, M., Shahbaz, M., Batool Qaisarani, T., Hanif Mughal, M., Salehi, B., Martorell, M., & Sharifi-Rad, J. (2020). Apigenin as an anticancer agent. Phytotherapy Research, 34(8), 1812-1828. DOI: 10.1002/ptr.6647
• İzol, E. (2016). Bazı Allium (Yabani Sarımsak) Türlerinin Aflatoksin, Ağır Metal ve Sekonder Metabolit İçeriklerinin ICP-MS ve LC-MS/MS İle Belirlenmesi ve Biyolojik Aktivitelerinin İncelenmesi. Yüksek Lisans, Dicle Üniversitesi, Fen Bilimleri Enstitüsü, Diyarbakır, Türkiye. https://acikerisim.dicle.edu.tr/items/052b25b8-fb19- 4104-b4cc-5963527fe017
• Magnani, C., Isaac, V.L.B., Correa, M.A., & Salgado, H.R.N. (2014). Caffeic acid: A review of its potential use in medications and cosmetics. Analytical Methods, 6(10), 3203-3210. DOI: 10.1039/C3AY41807C
• Mnayer, D., Fabiano-Tixier, A.-S., Petitcolas, E., Hamieh, T., Nehme, N., Ferrant, C., Fernandez, X., & Chemat, F. (2014). Chemical composition, antibacterial and antioxidant activities of six essentials oils from the Alliaceae family. Molecules, 19(12), 20034-20053. DOI: 10.3390/molecules191220034
• Ndoye Foe, F.M.-C., Tchinang, T.F.K., Nyegue, A.M., Abdou, J.-P., Yaya, A.J.G., Tchinda, A.T., Essame, J.-L.O., & Etoa, F.-X. (2016). Chemical composition, in vitro antioxidant and anti-inflammatory properties of essential oils of four dietary and medicinal plants from Cameroon. BMC Complementary and Alternative Medicine, 16(1), 117. DOI: 10.1186/s12906-016-1096- y
• Özdenefe, M.S., Kayış, F.B., & Takcı, A.M. (2024). Pyrus elaeagrifolia ve Crithmum maritimum L. Meyve ve Yaprak-Gövde Özütlerinin Antibakteriyel, Antioksidan, Antidiyabetik Potansiyelleri ve Kimyasal Bileşimi. Journal of Anatolian Environmental and Animal Sciences, 9(4), Article 4. DOI: 10.35229/jaes.1511026
• Phan, A.D.T., Netzel, G., Chhim, P., Netzel, M.E., & Sultanbawa, Y. (2019). Phytochemical characteristics and antimicrobial activity of Australian grown garlic (Allium sativum L.) cultivars. Foods, 8(9), 358.
• Roleira, F.M., Varela, C.L., Costa, S.C., & Tavares-da-Silva, E.J. (2018). Phenolic derivatives from medicinal herbs and plant extracts: Anticancer effects and synthetic approaches to modulate biological activity. Studies in Natural Products Chemistry, 57, 115-156.
• Sak, S., Özdenefe, M.S., Erol, Ü.H., & Takcı, A.M. (2024). Total Chemical Components, Biological Activity and Chromatographic Analyzes of Styrax officinalis Fruit Extract. Journal of Anatolian Environmental and Animal Sciences, 9(3), Article 3. DOI: 10.35229/jaes.1511075
• Sharifi‐Rad, J., Hoseini‐Alfatemi, S. M., Sharifi‐Rad, M., & Setzer, W. N. (2015). Chemical Composition, Antifungal and Antibacterial Activities of Essential Oil from Lallemantia royleana ( Benth. In Wall.) B enth. Journal of Food Safety, 35(1), 19-25. DOI: 10.1111/jfs.12139
• Simin, N., Orcic, D., Cetojevic-Simin, D., Mimica-Dukic, N., Anackov, G., Beara, I., Mitic-Culafic, D., & Bozin, B. (2013). Phenolic profile, antioxidant, anti- inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae). LWT-Food Science and Technology, 54(1), 139-146.
• Takım, K., Kutlu, T., Karaaslan, M.G., & Yılmaz, M.A. (2018). Tunceli Dağ Sarımsağının (Allium tuncelianum) Rat Kalp Dokusu Antioksidan Enzim Düzeylerine Etkisi ve Fenolik Bileşenlerinin Karakterizasyonu. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 21(5), 632-643.
• Uğurlu, S., & Bakkalbaşı, E. (2023). Yeşil Cevizlerden Ultrason Destekli Ekstraksiyon Yöntemiyle Fenolik Bileşiklerin Eldesi. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 28(1), Article 1. DOI: 10.53433/yyufbed.1150133
• Wang, W., Jia, Q., Mukerjee, S., & Chen, S. (2019). Recent Insights into the Oxygen-Reduction Electrocatalysis of Fe/N/C Materials. ACS Catalysis, 9(11), 10126-10141. DOI: 10.1021/acscatal.9b02583
• Yao, Y., Rao, C., Zheng, G., & Wang, S. (2019). Luteolin suppresses colorectal cancer cell metastasis via regulation of the miR‑384/pleiotrophin axis. Oncology Reports, 42(1), 131-141. DOI: 10.3892/or.2019.7136
• Yolbaş, İ. (2024a). Bellevalia pseudolongipes Plant: Comprehensive Analysis of the Elemental Composition and Total Phenolic and Flavonoid Contents. Black Sea Journal of Engineering and Science, 7(6), Article 6. DOI: 10.34248/bsengineering.1525176
• Yolbaş, İ. (2024b). Phenolic Compound Content and Antioxidant Activity of Rheum ribes Shells. Journal of Chemistry, 2024(1), 9151180. DOI: 10.1155/2024/9151180
• Yolbaş, İ. (2024c). Phytochemical profiling and antioxidant activity assessment of Bellevalia pseudolongipes via liquid chromatography-high-resolution mass spectrometry. PeerJ, 12, e18046. DOI: 10.7717/peerj.18046
• Yünlü, S., & Kır, E. (2016). Soğan (Allium cepa) ve sarımsaktaki (Allium sativum) bazı fenolik bileşiklerin HPLC yöntemiyle tayin edilmesi. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 20(3), 566-574.
• Zhang, X.-A., Zhang, S., Yin, Q., & Zhang, J. (2015). Quercetin induces human colon cancer cells apoptosis by inhibiting the nuclear factor-kappa B Pathway. Pharmacognosy Magazine, 11(42), 404-409. DOI: 10.4103/0973-1296.153096