Türkiye'de gökkuşağı alabalığı yumurtalarından izole edilen Saprolegnia ferax'ın moleküler teşhisi ve Hypericum perforatum ve Zingiber officinale uçucu yağlarının antifungal aktivitesi perforatum ve Zingiber officinale uçucu yağlarına duyarlılığı

Yıl 2025, Cilt: 10 Sayı: 4, 392 - 400, 31.07.2025

Öznur Özil , Ergi Bahrioğlu , Halit Bayrak

https://doi.org/10.35229/jaes.1656489

Öz

Bu çalışmada gökkuşağı alabalığı (Oncorhynchus mykiss) yumurtalarından izole edilen Saprolegnia ferax türü patojenik mantar türü Türkiye’de ilk defa moleküler yöntemlerle teşhis edilmiştir. Ayrıca çalışmada, teşhis edilen S. ferax suşunun Hypericum perforatum (kantaron) ve Zingiber officinale (zencefil) uçucu yağlarına karşı duyarlılığı belirlenmiştir. İzole edilen mantar türünün moleküler teşhisi amacıyla ITS1-ITS4 primerleri ile DNA barkotlama yapılmıştır. Filogenetik analiz yapılması amacıyla NCBI genbank veri tabanından temin edilen DNA dizileri filogenetik ağaç oluşturulması amacıyla kullanılmıştır. Uçucu yağların antifungal aktivitelerinin belirlenmesi amacıyla disk difüzyon ve tüp dilüsyon yöntemleri kullanılmıştır. Zencefil uçucu yağı, 15,62 µl/ml'lik Minimum İnhibitör Konsantrasyonu (MIC) ve 250 µl/ml’lik Minimum Letal Konsantrasyonu (MLC) ile antifungal etki gösterirken, kantaron uçucu yağının MIC değeri 125 µl/ml ve MLC değeri 1000 µl/ml olarak bulunmuştur. Bulgular, özellikle zencefil uçucu yağının S. ferax'a karşı etkili bir doğal antifungal ajan olarak değerlendirilebileceğini göstermektedir.

Anahtar Kelimeler

Antifungal aktivite , Disk difüzyon , ITS bölgesi , Tıbbi bitki , Tüp dilüsyon

Molecular identification of Saprolegnia ferax isolated from rainbow trout eggs in Turkey and antifungal activity of Hypericum perforatum and Zingiber officinale essential oilso essential oils of Hypericum perforatum and Zingiber officinale

Öz

In this study, the pathogenic fungus Saprolegnia ferax isolated from rainbow trout (Oncorhynchus mykiss) eggs was identified using the molecular methods for the first time in Turkey. In addition, the susceptibility of the identified S. ferax strain to essential oils of Hypericum perforatum (St. John's wort) and Zingiber officinale (ginger) was determined in the study. DNA barcoding was performed with ITS1-ITS4 primers for molecular identification of the isolated fungal species. DNA sequences obtained from the NCBI GenBank database were used to construct a phylogenetic tree for further analysis. The disk diffusion and tube dilution methods were used to determine the antifungal activities of essential oils. While ginger essential oil showed antifungal activity with a Minimum Inhibitory Concentration (MIC) of 15.62 µl/ml and Minimum Lethal Concentration (MLC) of 250 µl/ml, the MIC value of St. John's wort essential oil was found to be 125 µl/ml, the MLC value was 1000 µl/ml. The findings indicate that ginger essential oil may be considered an effective natural antifungal agent against S. ferax.

Anahtar Kelimeler

Antifungal activity , Disc diffusion , ITS region , Medicinal herbs , Tube dilution

Kaynakça

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