Molecular identification of Saprolegnia ferax isolated from rainbow trout eggs in Turkey and antifungal activity of Hypericum perforatum and Zingiber officinale essential oilso essential oils of Hypericum perforatum and Zingiber officinale

Öznur Özil; Ergi Bahrioğlu; Halit Bayrak

doi:10.35229/jaes.1656489

TR EN

Türkiye'de gökkuşağı alabalığı yumurtalarından izole edilen Saprolegnia ferax'ın moleküler teşhisi ve Hypericum perforatum ve Zingiber officinale uçucu yağlarının antifungal aktivitesi perforatum ve Zingiber officinale uçucu yağlarına duyarlılığı

Öz

Bu çalışmada gökkuşağı alabalığı (Oncorhynchus mykiss) yumurtalarından izole edilen Saprolegnia ferax türü patojenik mantar türü Türkiye’de ilk defa moleküler yöntemlerle teşhis edilmiştir. Ayrıca çalışmada, teşhis edilen S. ferax suşunun Hypericum perforatum (kantaron) ve Zingiber officinale (zencefil) uçucu yağlarına karşı duyarlılığı belirlenmiştir. İzole edilen mantar türünün moleküler teşhisi amacıyla ITS1-ITS4 primerleri ile DNA barkotlama yapılmıştır. Filogenetik analiz yapılması amacıyla NCBI genbank veri tabanından temin edilen DNA dizileri filogenetik ağaç oluşturulması amacıyla kullanılmıştır. Uçucu yağların antifungal aktivitelerinin belirlenmesi amacıyla disk difüzyon ve tüp dilüsyon yöntemleri kullanılmıştır. Zencefil uçucu yağı, 15,62 µl/ml'lik Minimum İnhibitör Konsantrasyonu (MIC) ve 250 µl/ml’lik Minimum Letal Konsantrasyonu (MLC) ile antifungal etki gösterirken, kantaron uçucu yağının MIC değeri 125 µl/ml ve MLC değeri 1000 µl/ml olarak bulunmuştur. Bulgular, özellikle zencefil uçucu yağının S. ferax'a karşı etkili bir doğal antifungal ajan olarak değerlendirilebileceğini göstermektedir.

Anahtar Kelimeler

Antifungal aktivite , Disk difüzyon , ITS bölgesi , Tıbbi bitki , Tüp dilüsyon

Molecular identification of Saprolegnia ferax isolated from rainbow trout eggs in Turkey and antifungal activity of Hypericum perforatum and Zingiber officinale essential oilso essential oils of Hypericum perforatum and Zingiber officinale

Abstract

In this study, the pathogenic fungus Saprolegnia ferax isolated from rainbow trout (Oncorhynchus mykiss) eggs was identified using the molecular methods for the first time in Turkey. In addition, the susceptibility of the identified S. ferax strain to essential oils of Hypericum perforatum (St. John's wort) and Zingiber officinale (ginger) was determined in the study. DNA barcoding was performed with ITS1-ITS4 primers for molecular identification of the isolated fungal species. DNA sequences obtained from the NCBI GenBank database were used to construct a phylogenetic tree for further analysis. The disk diffusion and tube dilution methods were used to determine the antifungal activities of essential oils. While ginger essential oil showed antifungal activity with a Minimum Inhibitory Concentration (MIC) of 15.62 µl/ml and Minimum Lethal Concentration (MLC) of 250 µl/ml, the MIC value of St. John's wort essential oil was found to be 125 µl/ml, the MLC value was 1000 µl/ml. The findings indicate that ginger essential oil may be considered an effective natural antifungal agent against S. ferax.

Keywords

Antifungal activity , Disc diffusion , ITS region , Medicinal herbs , Tube dilution

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Balık Zararlıları ve Hastalıkları

Bölüm

Araştırma Makalesi

Yazarlar

Öznur Özil ^*
0000-0002-7863-2943
Türkiye

Ergi Bahrioğlu
0000-0003-3707-337X
Türkiye

Halit Bayrak
0000-0003-3573-6440
Türkiye

Erken Görünüm Tarihi

15 Temmuz 2025

Yayımlanma Tarihi

31 Temmuz 2025

Gönderilme Tarihi

12 Mart 2025

Kabul Tarihi

18 Haziran 2025

Yayımlandığı Sayı

Yıl 2025 Cilt: 10 Sayı: 4

DOI

https://doi.org/10.35229/jaes.1656489

IZ

https://izlik.org/JA44BH99UH

APA

Özil, Ö., Bahrioğlu, E., & Bayrak, H. (2025). Molecular identification of Saprolegnia ferax isolated from rainbow trout eggs in Turkey and antifungal activity of Hypericum perforatum and Zingiber officinale essential oilso essential oils of Hypericum perforatum and Zingiber officinale. Journal of Anatolian Environmental and Animal Sciences, 10(4), 392-400. https://doi.org/10.35229/jaes.1656489

JAES, 2016- 11.yıl

Türkiye'de gökkuşağı alabalığı yumurtalarından izole edilen Saprolegnia ferax'ın moleküler teşhisi ve Hypericum perforatum ve Zingiber officinale uçucu yağlarının antifungal aktivitesi perforatum ve Zingiber officinale uçucu yağlarına duyarlılığı

Öz

Anahtar Kelimeler

Molecular identification of Saprolegnia ferax isolated from rainbow trout eggs in Turkey and antifungal activity of Hypericum perforatum and Zingiber officinale essential oilso essential oils of Hypericum perforatum and Zingiber officinale

Abstract

Keywords

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster