Evaluation of Bee Venom Induced Toxicity: Toxicity and Management

Sıdıka Genç; Kübra Karabulut; Esmanur Niğde; Şevval Büyükgöçmen; Ali Taghizadehghalehjoughi

doi:10.35229/jaes.1658697

EN TR

Evaluation of Bee Venom Induced Toxicity: Toxicity and Management

Abstract

Inflammation and increased cellular ROS levels caused by bee venom can increase formation of necrotic tissue and allergies. Many methods are used for bee venom induced toxicity. Two agents with therapeutic effects and pharmacological value have been included in these treatment options to decrease induced toxicity. L-tryptophan causes a decrease in the level of inflammation and the amount of ROS. Similarly, Amygdalin, which also targets the mTOR/AKT pathway and reduces inflammation, causes a decrease in FAK, ILK and β-catenin concentrations by inhibiting the expression of β1 and β4 integrins. Application of high doses of bee venom causes sensitization of nociceptors by activating TRPV1 via PLA2 cascade, which contains mellitin. This may result in pain and inflammation. We aimed to examine the toxic effects of bee venom by creating a wound model in the fibroblast cell line. After a linear wound was opened, the cells were exposed to bee venom (25 mg/ml) for 15 minutes. L-tryptophan and Vit B17 doses were applied at the end of 15 minutes. After 24 hours of incubation, wound healing was visualized and cell viability and oxidative damage tests were performed. The results showed that especially BV+ B17 + LT had a 60% effect on viability compared to the bee venom control group, resulting in wound closure. It was also determined that cellular ROS level decreased. All these results show that the combination of L-tryptophan and amygdalin has therapeutic efficacy on difficult-to-heal wounds.

Keywords

Bee Venom , L-Tryptophan , Toxicity; , B17 , NO

Arı Zehiri Kaynaklı Toksisitenin Değerlendirilmesi: Toksisite ve Yönetim

Öz

Arı zehrinin neden olduğu iltihaplanma ve artan hücresel ROS seviyeleri nekrotik doku oluşumunu ve alerjileri artırabilir. Arı zehri kaynaklı toksisite için birçok tedavi yöntemi kullanılmaktadır. İndüklenen toksisiteyi azaltmak için seçeneklerin terapötik etkileri ve farmakolojik değeri olan iki ajan dahil edilmiştir. L-triptofan iltihaplanma seviyesinde ve ROS miktarında azalmaya neden olur. Benzer şekilde mTOR/AKT yolunu da hedef alan ve iltihaplanmayı azaltan Amigdalin, β1 ve β4 integrinlerinin ekspresyonunu inhibe ederek FAK, ILK ve β-katenin konsantrasyonlarında azalmaya neden olur. Yüksek dozda arı zehri uygulaması, mellitin içeren PLA2 kaskadı aracılığıyla TRPV1'i aktive ederek nosiseptörlerin duyarlılaşmasına neden olur. Bu durum ağrı ve iltihaplanmaya neden olabilir. Fibroblast hücre hattında bir yara modeli oluşturarak arı zehrinin toksik etkilerini incelemeyi amaçladık. Doğrusal bir yara açıldıktan sonra hücreler 15 dakika boyunca arı zehrine (25 mg/ml) maruz bırakıldı. 15 dakikanın sonunda L-triptofan ve Vit B17 dozları uygulandı. 24 saatlik inkübasyondan sonra yara iyileşmesi görüntülendi ve hücre canlılığı ve oksidatif hasar testleri yapıldı. Sonuçlar özellikle BV+ B17 + LT dozunun arı zehri kontrol grubuna göre canlılık üzerinde %60 etkiye sahip olduğunu ve yaranın kapandığını gösterdi. Ayrıca hücresel ROS düzeyinin azaldığı belirlendi. Tüm bu sonuçlar L-triptofan ve amigdalin kombinasyonunun iyileşmesi zor yaralarda terapötik etkinliğe sahip olduğunu göstermektedir.

Anahtar Kelimeler

Arı Zehiri , Toksisite , L-Triptofan , B17 , NO

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Veteriner Hekimlik

Bölüm

Araştırma Makalesi

Yazarlar

Sıdıka Genç
0000-0003-0000-5103
Türkiye

Kübra Karabulut
0009-0002-7052-6086
Türkiye

Esmanur Niğde
0009-0004-6007-472X
Türkiye

Şevval Büyükgöçmen
0009-0005-7680-363X
Türkiye

Ali Taghizadehghalehjoughi ^*
0000-0002-3506-0324
Türkiye

Erken Görünüm Tarihi

31 Temmuz 2025

Yayımlanma Tarihi

31 Temmuz 2025

Gönderilme Tarihi

15 Mart 2025

Kabul Tarihi

21 Temmuz 2025

Yayımlandığı Sayı

Yıl 2025 Cilt: 10 Sayı: 4

DOI

https://doi.org/10.35229/jaes.1658697

IZ

https://izlik.org/JA86RC48XE

APA

Genç, S., Karabulut, K., Niğde, E., Büyükgöçmen, Ş., & Taghizadehghalehjoughi, A. (2025). Evaluation of Bee Venom Induced Toxicity: Toxicity and Management. Journal of Anatolian Environmental and Animal Sciences, 10(4), 514-520. https://doi.org/10.35229/jaes.1658697

JAES, 2016- 11.yıl

Evaluation of Bee Venom Induced Toxicity: Toxicity and Management

Abstract

Keywords

Arı Zehiri Kaynaklı Toksisitenin Değerlendirilmesi: Toksisite ve Yönetim

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

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