Analysis of Gut Microbiota and Assessment of Environmental Health in Western Anatolian Vole (Microtus lydius Blackler, 1916)

Yıl 2025, Cilt: 10 Sayı: 4, 471 - 477, 31.07.2025

Tuba Yağcı , Gözde Ayseçkin

https://doi.org/10.35229/jaes.1661557

Öz

The significant risk posed by zoonotic pathogens to humans increases the need to better understand how these pathogens are maintained and transmitted within ecosystems. Microtus (voles), one of the important members of wildlife, are widely distributed in agricultural fields and can directly interact with humans through farmers or agricultural products. Microbiological studies on this genus have identified certain pathogenic bacteria. However, the microbiota of free-living voles in nature also includes members with significant probiotic effects. In this study, the gut microbiota of Microtus lydius, a species widely distributed in Western Anatolia, was characterized for the first time through DNA isolation from fecal samples and the use of 16S rRNA next-generation sequencing technology. The detected bacterial groups were examined across all taxonomic categories. According to the microbial analysis results of the species, the dominant bacteria in the gut microbiota are probiotic in nature, reflecting the species' dietary characteristics. Pathogenic bacteria, on the other hand, are present at low abundance and contribute to species diversity. The microbial records identified for Microtus lydius provide valuable insights for assessing the infectious risks of this species, which interacts with humans in the wild, as well as for understanding the probiotic health effects in social behavior models and contributing to phylogenetic research.

Anahtar Kelimeler

microtus , wildlife , microbiota

Etik Beyan

Ethical approval is not required.

Destekleyen Kurum

2209-A Tübitak

Proje Numarası

1919B012105702

Teşekkür

This research was supported by TÜBİTAK 2209-A.

Batı Anadolu Tarla Faresi (Microtus lydius Blackler, 1916)’nde Bağırsak Mikrobiyotası Analizi ve Çevresel Sağlık Değerlendirmesi

Öz

Zoonotik patojenlerin insanlar için önemli bir risk oluşturması, bu patojenlerin ekosistemlerde nasıl korunduğu ve bulaştığı hakkında daha fazla bilgi edinme gerekliliğini artırmaktadır. Yaban hayatının önemli üyelerinden biri olan Microtus (tarla fareleri), tarlalarda geniş bir yayılışa sahiptir ve doğrudan çiftçilerle veya tarım ürünleri aracılığıyla insanlarla etkileşime girebilmektedir. Bu cinse ait mikrobiyolojik çalışmalarda bazı patojenik bakteriler tespit edilmiştir. Bununla birlikte, doğada serbest yaşayan tarla farelerinin mikrobiyotası, önemli probiyotik etkilere sahip üyeler de içermektedir. Bu araştırmada Batı Anadolu’ da geniş bir yayılışa sahip Microtus lydius türünün bağırsak mikrobiyotası, dışkı örneklerinden DNA izolasyonu yapılarak ve 16S rRNA yeni nesil dizileme tekniği kullanılarak ilk kez karakterize edilmiştir. Tespit edilen bakteri grupları, tüm taksonomik kategorilerde incelenmiştir. Türün mikrobiyal analiz sonuçlarına göre, bağırsak mikrobiyotasındaki baskın bakteriler, türün beslenme özelliklerini yansıtacak şekilde ve probiyotik niteliktedir. Patojenik özellikteki bakteriler ise düşük yoğunlukta ve tür çeşitliliğine katkıda bulunmaktadır. Araştırma verileri, yaban hayatında insanlarla etkileşime giren bu türün enfeksiyonel risk değerlendirmesi, sosyal davranış modellerindeki probiyotik sağlık etkileri ve filogenetik araştırmalar için önemli bir katkı sağlayacak niteliktedir.

Anahtar Kelimeler

Microtus , Yaban Hayatı , Mikrobiyota

Etik Beyan

Etik kurul onayı gerekmemektedir.

Destekleyen Kurum

Tübitak 2209-A

Proje Numarası

1919B012105702

Teşekkür

Bu araştırma, TÜBİTAK 2209-A tarafından desteklenmiştir.

Kaynakça

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