Qualitative Phytochemical Screening, Total Phenolics Content, In Vitro Antioxidant Activity and Antibacterial Activities of Arbutus unedo L. Leaves

Ahmet Beyatli

doi:10.35229/jaes.1669409

EN TR

Qualitative Phytochemical Screening, Total Phenolics Content, In Vitro Antioxidant Activity and Antibacterial Activities of Arbutus unedo L. Leaves

Abstract

The leaves of Arbutus unedo traditionally were used in Mediterranean folk medicine. In this study phytochemical composition, phenolic content, and bioactivities were investigated. The phytochemical screening revealed the presence of multiple bioactive compounds, including flavonoids, tannins, terpenoids, steroids, and glycosides, distributed across different fractions. Quantitative analysis demonstrated that the ethanolic extract contained substantially greater amounts of both total phenolics and flavonoids compared to other extracts (738.43 ± 5.11 mg GAE/g and 98.14 ± 2.13 mg CE/g, respectively). In vitro antioxidant activity, assessed through DPPH, ABTS, and FRAP assays, the ethyl acetate fraction demonstrated the strongest antioxidant effects in every assay. Moreover, ethyl acetate fraction showed the strongest antibacterial activity, with the largest inhibition zones against S. aureus (15.25±1.10 mm) and P. aeruginosa (17.75±1.33 mm) and the lowest MIC values (78–156 µg/mL). The ethanol extract exhibited moderate activity, while the hexane, chloroform, and aqueous fractions were less effective, with MICs >625 µg/mL for most strains. The results demonstrate that A. unedo leaf extracts exhibit significant antioxidant and antimicrobial properties, validating their traditional medicinal applications and suggesting their potential as a natural alternative for therapeutic or preservative purposes.

Keywords

Antibacterial activity , Antioxidant activity , Arbutus unedo , Phenolics , Phytochemical screening

Arbutus unedo L. Yapraklarının Kalitatif Fitokimyasal Taraması, Toplam Fenolikler İçeriği, İn Vitro Antioksidan Aktivitesi ve Antibakteriyel Etkileri

Öz

Arbutus unedo yaprakları geleneksel olarak Akdeniz halk hekimliğinde kullanılmıştır. Bu çalışmada fitokimyasal bileşim, fenolik içerik ve biyoaktiviteler araştırılmıştır. Çeşitli fraksiyonların fitokimyasal analizinde, flavonoidler, tanenler, terpenoidler, steroidler ve glikozitler belirlenmiştir. Toplam fenolik içerik ve toplam flavonoid içeriği etanol ekstresinde önemli ölçüde daha yüksek (sırasıyla 738,43 ± 5,11 mg GAE/g ve 98,14 ± 2,13 mg CE/g) bulunmuştur. DPPH, ABTS ve FRAP analizleri ile değerlendirilen in vitro antioksidan aktivitede, etil asetat fraksiyonu tüm analizlerde en güçlü antioksidan etkiyi göstermiştir. Ayrıca etil asetat fraksiyonunda S. aureus'a (15,25 ± 1,10 mm) ve P. aeruginosa'ya (17,75 ± 1,33 mm) karşı en büyük inhibisyon bölgeleri ve en düşük MİK değerleri (78–156 µg/mL) göstererek en güçlü antibakteriyel aktivite tespit edilmiştir. Etanol ekstresi orta düzeyde aktivite gösterdi, hekzan, kloroform ve sulu fraksiyonlar ise daha az etki göstermiştir ve çoğu suşa karşı MİK'ler > 625 µg/mL olarak belirlenmiştir. Bulgular, A. unedo yapraklarının geleneksel ve gelecekteki doğal bir antimikrobiyal ve antioksidan ajan olarak potansiyelini vurgulamaktadır.

Anahtar Kelimeler

Antibakteriyel aktivite , Antioksidan aktivite , Arbutus unedo , Fenolikler , Fitokimyasal tarama

Kaynakça

Baytop, T. (1999). Curing with plants in Turkey, in the past and today (Türkiye ‘de bitkiler ile tedavi, geçmişte ve bugün). Nobel Medical Books, Capa, İstanbul, 299p.
Benzie, I.F.F., & Strain, J.J. (1996). The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay. Analytical Biochemistry, 239(1), 70-76. DOI: 10.1006/abio.1996.0292
Beyatli, A., Peker, E.G.G., Gül, N., & Cevher, Ş.C. (2022). Morin (2′, 3, 4′, 5, 7-Pentahydroxyflavon) antioxidant effect in streptozotocin-ınduced diabetic rat brain and heart tissues. Journal of Anatolian Environmental and Animal Sciences, 7(3), 257-262. DOI: 10.35229/jaes.1103000
Chanda, S., & Dave, R. (2009). In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: An overview. African Journal of Microbiology Research, 3(13), 981-996.
Chua, K.W., Sia, C.M., Akowuah, G.A., Samuagam, L., & Yim, H.S. (2015). Antioxidative properties and HPLC profile of chloroform fraction from ethanolic extract of the peel of Citrus hystrix. Chiang Mai Journal of Science, 42, 173-184.
CLSI. (2008). Performance standards for antimicrobial susceptibility testing. (Clinical and Laboratory Standards Institute 28th ed. CLSI supplement M100). Wayne, PA.
Dewanto, V., Wu, X., Adom, K.K., & Liu, R.H. (2002). Thermal Processing Enhances the Nutritional Value of Tomatoes by Increasing Total Antioxidant Activity. Journal of Agricultural and Food Chemistry, 50(10), 3010-3014. DOI: 10.1021/jf0115589
Di Lorenzo, R., Ferraro, M. G., Carrera, C., Iazzetti, F., Chinchilla, N., Maisto, M., Aliaño-González, M.J., Piccolo, V., Romano, A., Ricci, L., Medronho, B., Marzocchi, A., Piccolo, M., Tenore, G.C., Irace, C., & Laneri, S. (2025). Valorization of Arbutus unedo L. Pomace: Exploring the recovery of bioactive phenolic compounds from distillation by-products. Antioxidants, 14(3), 278. DOI: 10.3390/antiox14030278
Endalew, S.A., Taddese, M.G., & Muhammed, M. (2024). Evaluation of antioxidant and antibacterial properties of dehydrocostus lactone isolated from Echinops kebericho root. Health Science Reports, 7(3), e1990. DOI: 10.1002/hsr2.1990
El Haouari, M., Assem, N., Changan, S., Kumar, M., Daştan, S.D., Rajkovic, J., Taheri, Y., & Sharifi-Rad, J. (2021). An insight into phytochemical, pharmacological, and nutritional properties of Arbutus unedo L. from Morocco. Evidence-Based Complementary and Alternative Medicine, 2021(1). DOI: 10.1155/2021/1794621

Ertürk, Ö. (2006). Antibacterial and antifungal activity of ethanolic extracts from eleven spice plants. Biologia, 61(3), 275-278. DOI: 10.2478/s11756- 006-0050-8
Halim, A.P., Wijayanti, N., Hidayati, L., & Nuringtyas, T.R. (2022). Antioxidant activity evaluation of agarwood Aquilaria malaccensis Lamk. leaves extract using DPPH, FRAP and ABTS assays. 7th International Conference on Biological Science (pp. 18-25). Atlantis Press.
Harborne, A.J. (1998). Phytochemical methods a guide to modern techniques of plant analysis. Springer Science & Business Media.
Haruni, M.J., Parvin, M.S., Munira, S., & Islam, M.E. (2025). Phytochemical profile and evaluation of the antioxidant, anticancer, and antimicrobial potential of Artocarpus lacucha bark extracts: In vitro and ın silico studies. Journal of Food Biochemistry, 2025(1), 8016598. DOI: 10.1155/jfbc/8016598
Hernandez, M.M., Heraso, C., Villarreal, M.L., Vargas-Arispuro, I., & Aranda, E. (1999). Biological activities of crude plant extracts from Vitex trifolia L.(Verbenaceae). Journal of Ethnopharmacology, 67(1), 37-44. DOI: 10.1016/S0378-8741(99)00041-0
Hossain, M.A., Al-Hdhrami, S.S., Weli, A.M., AlRiyami, Q., & Al-Sabahi, J.N. (2014). Isolation, fractionation and identification of chemical constituents from the leaves crude extracts of Mentha piperita L grown in Sultanate of Oman. Asian Pacific Journal of Tropical Biomedicine, 4, S368-S372. DOI: 10.12980/APJTB.4.2014C1051
Huang, J., Zaynab, M., Sharif, Y., Khan, J., Al-Yahyai, R., Sadder, M., … & Li, S. (2024). Tannins as antimicrobial agents: Understanding toxic effects on pathogens. Toxicon, 247, 107812. DOI: 10.1016/j.toxicon.2024.107812
Ibrahim, A.M., Lawal, B., Abubakar, A.N., Tsado, N.A., Kontagora, G.N., Gboke, J.A., & Berinyuy, E.B. (2017). Antimicrobial and free radical scavenging potentials of N-hexane and ethyl acetate fractions of Phyllanthus Fraternus. Nigerian Journal of Basic and Applied Sciences, 25(2), 6-11.
IUCN. (2017). IUCN Red List of Threatened Species: Arbutus unedo. Retrieved March 28, 2025, from https://www.iucnredlist.org/species/202930/6807 6133
Khanum, R., Jahangir, M., Abbasi, M.A., Mazhar, F., Kausar, S., Riaz, T., & Ajaib, M. (2013). Phytochemical screening and antioxidant evaluations of different fractions of Argyrolobium roseum. Asian Journal of Chemistry, 25(13), 7485-7489.
Klimek, P., Dervic, E., Friesenbichler, K., Gerschberger, M., & Yang, L. (2023). The anatomy of the current antibiotics shortage. Vienna, Austria: Supply Chain Intelligence Institute. Retrieved from https://papers.ssrn.com/sol3/papers.cfm?abstract _id=4451526
Koyu, H., Koyu, E.B., Demir, S., & Baykan, Ş. (2019). Arbutus unedo L.(Kocayemiş). Türk Farmakope Dergisi, 4(3), 29-51.
Markovinović, A.B., Karačonji, I.B., Jurica, K., Lasić, D., Babojelić, M.S., Duralija, B., Žlabur, J.S., Putnik, P., & Kovačević, D.B. (2022).
Strawberry tree fruits and leaves (Arbutus unedo L.) as raw material for sustainable functional food processing: A review. Horticulturae, 8(10), 881. DOI: 10.3390/horticulturae8100881
Mariem, S., Hanen, F., Inès, J., Mejdi, S., & Riadh, K. (2014). Phenolic profile, biological activities and fraction analysis of the medicinal halophyte Retama raetam. South African Journal of Botany, 94, 114-121. DOI: 10.1016/j.sajb.2014.06.010
Miguel, M.G., Faleiro, M.L., Guerreiro, A.C., & Antunes, M.D. (2014). Arbutus unedo L.: Chemical and biological properties. Molecules, 19(10), 15799-15823. DOI: 10.3390/molecules191015799
Morales, D. (2022). Use of strawberry tree (Arbutus unedo) as a source of functional fractions with biological activities. Foods, 11(23), 3838. DOI: 10.3390/foods11233838
Murray, C.J.L., Ikuta, K.S., Sharara, F., Swetschinski, L., Aguilar, G.R., Gray, A., …& Naghavi, M. (2022). Global burden of bacterial antimicrobial resistance in 2019: A systematic analysis. The Lancet, 399(10325), 629-655. DOI: 10.1016/S0140-6736(21)02724-0
Nikkon, F., Saud, Z.A., Rahman, M.H. & Haque, Md. E. (2003). In vitro antimicrobial activity of the compound isolated from chloroform extract of Moringa oleifera Lam. Pakistan Journal of Biological Sciences, 6(22), 1888-1890.
Pandey, A.K., Cohn, J., Nampoothiri, V., Gadde, U., Ghataure, A., Kakkar, A. K., … & Charani, E. (2025). A systematic review of antibiotic drug shortages and the strategies employed for managing these shortages. Clinical Microbiology and Infection, 31(3), 345-353. DOI: 10.1016/j.cmi.2024.09.023
Radulovic, N.S., Blagojevic, P.D., Stojanovic-Radic, Z.Z. & Stojanovic, N.M. (2013). Antimicrobial plant metabolites: Structural diversity and mechanism of action. Current Medicinal Chemistry, 20(7), 932-952.
Rumpf, J., Burger, R. & Schulze, M. (2023). Statistical evaluation of DPPH, ABTS, FRAP, and FolinCiocalteu assays to assess the antioxidant capacity of lignins. International Journal of Biological Macromolecules, 233, 123470. DOI: 10.1016/j.ijbiomac.2023.123470
Tastekin, B., & Çiftci, G. (2023). Antioxidant capacity and antibacterial potential of rosehip (Rosa canina) fruits grown. Journal of Anatolian Environmental and Animal Sciences, 8(1), 103- 109. DOI: 10.35229/jaes.1240877
Tyler, V.E. (1993). Phytomedicines in Western Europe: Potential impact on herbal medicine in the United States. ACS Publications.
Wahid, N., Faida, R., Aabdousse, J., Boulli, A., & Bouda, S. (2019). Ethnobotanical uses and distribution status of Arbutus (Arbutus unedo L.) in Morocco. Ethnobotany Research and Applications, 18, 1-12. DOI: 10.32859/era.18.30
Xie, Y., Yang, W., Tang, F., Chen, X., & Ren, L. (2015). Antibacterial activities of flavonoids: Structureactivity relationship and mechanism. Current Medicinal Chemistry, 22(1), 132-149. DOI: 10.2174/0929867321666140916113443
Yoo, S., Kim, K., Nam, H., & Lee, D. (2018). Discovering health benefits of phytochemicals with integrated analysis of the molecular network, chemical properties and ethnopharmacological evidence. Nutrients, 10(8), 1042. DOI: 10.3390/nu10081042
Zhou, K., Su, L., & Yu, L. (2004). Phytochemicals and antioxidant properties in wheat bran. Journal of Agricultural and Food Chemistry, 52(20), 6108- 6114. DOI: 10.1021/jf049214g
Zitouni, H., Hssaini, L.H., Ouaabou, R., Viuda-Martos, M., Hernandez, F., Ercisli, S., … & Hanine, H. (2021). Functionnal and Technological Properties of Five Strawberry (Arbutus Unedo L.) Fruit as Bioactive Ingredients in Functional Foods. International Journal of Food Properties, 24(1), 380-399. DOI: 10.1080/10942912.2021.1883058

Ayrıntılar

Birincil Dil

İngilizce

Konular

Ekoloji (Diğer)

Bölüm

Araştırma Makalesi

Yazarlar

Ahmet Beyatli ^*
0000-0001-5225-6217
Türkiye

Erken Görünüm Tarihi

15 Eylül 2025

Yayımlanma Tarihi

30 Eylül 2025

Gönderilme Tarihi

3 Nisan 2025

Kabul Tarihi

5 Ağustos 2025

Yayımlandığı Sayı

Yıl 2025 Cilt: 10 Sayı: 5

DOI

https://doi.org/10.35229/jaes.1669409

IZ

https://izlik.org/JA66GC43UH

APA

Beyatli, A. (2025). Qualitative Phytochemical Screening, Total Phenolics Content, In Vitro Antioxidant Activity and Antibacterial Activities of Arbutus unedo L. Leaves. Journal of Anatolian Environmental and Animal Sciences, 10(5), 533-539. https://doi.org/10.35229/jaes.1669409

JAES, 2016- 11.yıl

Qualitative Phytochemical Screening, Total Phenolics Content, In Vitro Antioxidant Activity and Antibacterial Activities of Arbutus unedo L. Leaves

Abstract

Keywords

Arbutus unedo L. Yapraklarının Kalitatif Fitokimyasal Taraması, Toplam Fenolikler İçeriği, İn Vitro Antioksidan Aktivitesi ve Antibakteriyel Etkileri

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

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