Fusarium proliferatum: isolation, identification and evaluation of its association with the model insect Galleria mellonella (L.) (Lepidoptera: Pyralidae)

Pınar Güner; Aylin Er; Tülin Aşkun; Görkem Deniz Sönmez; Selin Meryem Şengül

doi:10.35229/jaes.1681652

TR EN

Fusarium proliferatum: izolasyonu, tanımlaması ve model Böcek Galleria mellonella (L.) (Lepidoptera: Pyralidae) ile ilişkisinin değerlendirilmesi

Öz

Fusarium cinsi, önemli bitki patojenleri ve mikotoksin üreticileri içermesiyle dikkat çekmesine rağmen birçok Fusarium türünün böcekleri kontrol etmede etkili olduğu ve yüksek ölüm oranlarına neden olma, hızlı etki ve bol sporlanma gibi tarımsal zararlı kontrolü için umut verici özellikler sergilediği bilinmektedir. Bu çalışmada, topraktan tuzak metodu ile izole edilen Fusarium proliferatum'un mikro-makro morfolojisi, DNA dizilimi ve model böcek Galleria mellonella üzerindeki etkisi araştırılmıştır. Morfolojik analizler, farklı agarlarda ayrıntılı koloni ve spor yapıları ortaya koymuştur. DNA analizi ile ITS ve CaM gen dizileri kullanılarak tür tanımlamasını doğrulanmıştır. F. proliferatum'un model böcek G. mellonella 'nın larval evresi uzattığı ve pupa süresini, ağırlığını ve toplam yumurta sayısını azalttığı gözlenmiştir. Çalışmamız fungusun G. mellonella 'nın gelişme ve üremesi üzerinde doza bağlı bir etkiye sahip olduğunu göstermektedir. Gelecekteki çalışmalar, gözlenen biyolojik etkilerden sorumlu ikincil metabolitlerin tanımlanmasına, hedef dışı organizmalar üzerindeki olası etkilerin değerlendirilmesi ve farklı çevre koşulları altında saha denemelerinin yürütülmesine odaklanmalıdır.

Anahtar Kelimeler

Fusarium proliferatum , fungal patojenite , Galleria mellonella , gelişim biyolojisi , izolasyon , tanımlama

Fusarium proliferatum: isolation, identification and evaluation of its association with the model insect Galleria mellonella (L.) (Lepidoptera: Pyralidae)

Abstract

Although the genus Fusarium is widely recognized for including significant plant pathogens and mycotoxin producers, many Fusarium species have also demonstrated promising potential for insect control. These species exhibit characteristics such as high mortality rates, rapid action, and prolific sporulation, making them attractive candidates for agricultural pest management. In this study, the micro and macromorphology, DNA sequencing, and the effects on the model insect Galleria mellonella of Fusarium proliferatum isolated from soil using the trap method were investigated. Morphological analyses revealed detailed colony and spore structures on various agar media. Species identification was confirmed through DNA analysis using ITS and CaM gene sequences. F. proliferatum was observed to prolong the larval stage of model ınsect G. mellonella while reducing pupal duration, weight, and total egg production. Our findings indicate that the fungus exerts a dose-dependent impact on the development and reproduction of G. mellonella. Future studies should focus on identifying secondary metabolites responsible for the observed biological effects, evaluating possible effects on non-target organisms, and conducting field trials under different environmental conditions.

Keywords

Fusarium proliferatum , Galleria mellonella , developmental biology , fungal pathogenicity , identification , isolation

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Hayvan Fizyolojisi - Ekofizyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Pınar Güner ^*
0000-0001-6922-7009
Türkiye

Aylin Er
0000-0002-8108-8950
Türkiye

Tülin Aşkun
0000-0002-2700-1965
Türkiye

Görkem Deniz Sönmez
0000-0002-3613-0195
Türkiye

Selin Meryem Şengül
0009-0002-1842-4525
Türkiye

Erken Görünüm Tarihi

15 Temmuz 2025

Yayımlanma Tarihi

31 Temmuz 2025

Gönderilme Tarihi

23 Nisan 2025

Kabul Tarihi

27 Haziran 2025

Yayımlandığı Sayı

Yıl 2025 Cilt: 10 Sayı: 4

DOI

https://doi.org/10.35229/jaes.1681652

IZ

https://izlik.org/JA38JD49GE

APA

Güner, P., Er, A., Aşkun, T., Deniz Sönmez, G., & Şengül, S. M. (2025). Fusarium proliferatum: isolation, identification and evaluation of its association with the model insect Galleria mellonella (L.) (Lepidoptera: Pyralidae). Journal of Anatolian Environmental and Animal Sciences, 10(4), 416-423. https://doi.org/10.35229/jaes.1681652

JAES, 2016- 11.yıl

Fusarium proliferatum: izolasyonu, tanımlaması ve model Böcek Galleria mellonella (L.) (Lepidoptera: Pyralidae) ile ilişkisinin değerlendirilmesi

Öz

Anahtar Kelimeler

Fusarium proliferatum: isolation, identification and evaluation of its association with the model insect Galleria mellonella (L.) (Lepidoptera: Pyralidae)

Abstract

Keywords

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

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