Transcriptional expression profiling of feline squamous cell carcinomas

Mahmut Sözmen; Yonca Betil Kabak; Sinem İnal; Fatma Betül Akça

doi:10.35229/jaes.1775035

TR EN

Kedi skuamöz hücreli karsinomlarının transkripsiyonel ekspresyon profili

Öz

Skuamöz hücreli karsinom (SCC), kedilerde en sık görülen malign kutanöz ve oral tümörlerdir. Kedi skuamöz hücreli karsinomlarında (FSCC) genomik değişiklikler kedilerde karakterize edilmemiştir. Bu çalışmanın amacı, mikroarray analizi ile doğal olarak ortaya çıkan FSCC vakalarında ekspresyonu önemli ölçüde değişen genleri belirlemektir. İki adet formalinle fikse edilmiş parafin gömülü (FFPE) FSCC vakası, GeneChipTM Feline Gene 1.0 ST Array ile analiz edildi. Mikroarray analizi, FSCC vakalarının 35.034 gen düzeyinde prob setinden 148 farklı şekilde ifade edilen gen barındırdığını ortaya koydu. Bu değişikliklerden 136 gen yukarı doğru düzenlenirken, diğer 12 gen aşağı doğru düzenlendi. Mikroarray analizi sonuçları, yukarı regüle edilen genlerin çoğunun onkojenik olduğunu ortaya koydu. FSCC gelişimine yol açan moleküler olaylarda rol oynayabilecek, ekspresyon seviyeleri önemli ölçüde yükselmiş genler (NCK1, PDE7A, UBAP2L, PPP2R5A ve CDK1) seçildi ve bölüm arşivlerinden alınan toplam 26 FFPE FSCC vakasında gerçek zamanlı PCR ile daha ayrıntılı olarak incelendi. Buna göre, bu 26 FFPE FSCC vakasında, NCK1, PDE7A, UBAP2L, PPP2R5A ve CDK1 genlerinin kat değişiklikleri sırasıyla 6,96, 2,58, 5,24, 55,13 ve 3,5 idi. Mikroarray analizi ve gerçek zamanlı PCR sonuçları, yukarı regüle edilen genlerin hücre döngüsünde rol oynadığını ve proliferasyonun da FSCC'nin gelişimine katkıda bulunabileceğini göstermektedir.

Anahtar Kelimeler

gen ifadesi , kedi , mikroarray , skuamöz hücreli karsinom.

Transcriptional expression profiling of feline squamous cell carcinomas

Abstract

Squamous cell carcinoma (SCC) is the most common malignant cutaneous and oral tumors occurring in cats. Genomic alterations in feline squamous cell carcinomas (FSCC) have not been characterized in cats. The objective of the present study was to determine genes of which expression was significantly altered in naturally occurring FSCC cases by microarray analysis. Two formalin fixed paraffin embedded (FFPE) FSCC cases were analyzed with GeneChipTM Feline Gene 1.0 ST Array. Microarray analysis revealed that FSCC cases harbored 148 differentially expressed genes out of 35,034 gene-level probe sets. Of these changes, 136 genes were up-regulated while the other 12 genes were down-regulated. Microarray analysis results revealed that most of the upregulated genes were oncogenic. Expression levels significantly elevated genes (NCK1, PDE7A, UBAP2L, PPP2R5A, and CDK1) which may have a role in the molecular events leading to the development of FSCC were selected and further examined by real-time PCR in a total of 26 FFPE FSCC cases retrieved from departmental archives. Accordingly, in those 26 FFPE FSCC cases, the fold changes for the NCK1, PDE7A, UBAP2L, PPP2R5A, and CDK1 genes were 6.96, 2.58, 5.24, 55.13, 3.5, respectively. Microarray analysis and real-time PCR results indicate that upregulated genes are involved in the cell cycle and proliferation may also contribute to the development of FSCC.

Keywords

cat , gene expression , microarray , squamous cell carcinoma

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Mahmut Sözmen ^*
0000-0001-7976-4051
Türkiye

Yonca Betil Kabak
0000-0002-3442-8377
Türkiye

Sinem İnal
0000-0002-2552-5159
Türkiye

Fatma Betül Akça
0000-0003-3030-3101
Türkiye

Erken Görünüm Tarihi

15 Kasım 2025

Yayımlanma Tarihi

30 Kasım 2025

Gönderilme Tarihi

1 Eylül 2025

Kabul Tarihi

20 Ekim 2025

Yayımlandığı Sayı

Yıl 2025 Cilt: 10 Sayı: 6

DOI

https://doi.org/10.35229/jaes.1775035

IZ

https://izlik.org/JA67JP62AG

APA

Sözmen, M., Kabak, Y. B., İnal, S., & Akça, F. B. (2025). Transcriptional expression profiling of feline squamous cell carcinomas. Journal of Anatolian Environmental and Animal Sciences, 10(6), 861-868. https://doi.org/10.35229/jaes.1775035

JAES, 2016- 11.yıl

Kedi skuamöz hücreli karsinomlarının transkripsiyonel ekspresyon profili

Öz

Anahtar Kelimeler

Transcriptional expression profiling of feline squamous cell carcinomas

Abstract

Keywords

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

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