The Isolation of Lactococcus garvieae from Eyes of Diseased Rainbow Trout (Oncorhynchus mykiss) with Exopthalmia
Abstract
In this study, fish were
sampled from 9 different fish farms in the dam lakes in the Eastern Black Sea
Region between January 2016 and September 2018. It was isolated twenty-two bacteria
isolates from eyes of diseased rainbow trout (Oncorhynchus mykiss). All
isolates identification was carried out by using conventional biochemical
tests, api 20 strep tests and PCR tests. ATCC 43921 reference strain was used
as a positive control. According to results of the api 20 strep test, it was
determined that 20 isolates from 22 bacterial isolates were Lactococcus garvieae. The PZR test was
confirmed by using the pLG-1 and pLG-2 reference genes (16S rRNA) specific for
the L. garvieae species. As a result
of the study, it was confirmed that there were 20 L. garvieae from 22 strains isolated from rainbow trout in the dam
lakes fish farms. The antibiotic susceptibility test results showed that L. garvieae isolates were resistant %100
to streptomycin, sulfamethoxazole and sulfamethoxazole+trimethoprim, 94.2% to
ampicillin, 72.5% to oxytetracycline, 55.07% to erythromycin and 43.5% to
oxolinic acid, 26.09% to enrofloxacin, % 20.3 doxycycline, 8,7% to amoxicillin
and florphenicol. The most effective antibiotic was found to be florfenicol,
amoxicillin, doxisicline and enrofloxacin.
Keywords
Kaynakça
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