Fasulye gelişiminde, Rhizoctonia solani B227'nin biyotik stresine karşı Trichoderma harzianum ID11C'nin biyokontrol etkinliği

Şengül Alpay Karaoğlu; Arif Bozdeveci; Müberra Pınarbaş; Gökhan Veyisoğlu; Cemre Tarakçı

doi:10.35229/jaes.555230

TR EN

Fasulye gelişiminde, Rhizoctonia solani B227'nin biyotik stresine karşı Trichoderma harzianum ID11C'nin biyokontrol etkinliği

Öz

Çalışmada çay toprağından izole edilen Trichoderma harzianum ID11C suşunun bazı enzim aktivitesi, ağır metallere toleransı, tohum (domates, mısır ve fasulye) çimlenmesi üzerine etkinliği ve fasulyede Rhizoctonia solani B227 (AG-4)'nin biyotik stresine karşı biyokontrol aktivitesinin araştırılması amaçlandı. T. harzianum ID11C suşunun bazı enzim aktiviteleri kromojenik yöntemle incelendi. Sonuçlar farklı konsantrasyonlarda ağır metal (Cu, Pb, Zn ve Cd) ve tuz toleransı olduğunu gösterdi.T. harzianum ID11C Rhizoctonia solani B227(AG4) izolatının fasulye gelişimine karşın patojenite testi ve T. harzianum ID11C suşunun tohum çimlenme başarısına olan etkinliği, su agar metodu ile incelendi. T. harzianum ID11C ve R. solani B227 varlığında fasulye gelişim parametreleri saksı deneyi ile araştırıldı. Fizyolojik (ana kök ve gövde uzunluğu, saçaklanma sayısı, yaprak sayısı, yaş ve kuru ağırlıkları) parametreler ölçüldü ve istatistiksel analizleri yapıldı.

Trichoderma harzianum ID11C suşu, yüksek konsantrasyonlarda ağır metallere (100-400 mM) ve tuza (250 mM) karşı toleranslıdır. Bitki gelişimini teşvik eden enzim aktivitelerinin var olduğu gözlendi. T. harzianum suşunun tohumların çimlenme başarısı üzerinde kontrole kıyasla olumsuz bir etkisinin olmadığı tespit edildi. Saksı deneyinde, kontrol ile R. solani B227 grupları birbirine kıyaslandığında, gövde ve ana kök uzunluğu, saçaklanma sayısı ve kökte lezyon skala değerleri ile yapılan istatistik analizi sonucu aralarında anlamlı farkın (p< 0.05, Tukey) olduğu belirlendi. R. solani B227 ve T. harzianum ID11D’nin ayrı ayrı fasulye gelişimi üzerine olan etkileri, kontrol grubuna göre iyi olmadığı belirlendi. Ancak ID11C ve B227+ID11C grupları B227 grubu ile kıyaslandığında, patojenik etkinliği azalttığı belirlendi.

R. solani B227 (AG4) izolatı, fasulyede (Zulbiye) patojeniteye neden olduğu, T. harzianum ID11C suşunun ise istatistiksel olarak olumlu yönde anlamlı bir fark oluşturmamasına rağmen, tüm parametrelerde patojeniteyi azalttığı belirlendi. T. harzianum ID11C suşunun fasulye tarımında Rhizoctonia solani’ye karşı biyokontrol ve bitki destekleyici ajan olarak kullanılabileceği sonucuna varıldı.

Anahtar Kelimeler

Trichoderma harzianum,Rhizoctonia solani,biyokontrol,bitki patojeni

The biocontrol activity of Trichoderma harzianum ID11C against to the biotic stress of Rhizoctonia solani B227 in bean development

Abstract

The aim of the study was to determine the efficacy of Trichoderma harzianum ID11C strain isolated from tea soil on germination of seeds (tomato, maize and bean) and to investigate the biocontrol efficacy against biotic stress of Rhizoctonia solani B227(AG4) in beans. Some enzyme activities of T. harzianum ID11C strain were investigated by chromogenic method. It was determined that it had tolerance to heavy metals (Cu, Pb, Zn and Cd) and salt in different concentrations. The results showed different concentration of some heavy metal(Cu, Pb, Zn ve Cd) resistance and salt tolerance. The pathogenicity test against the bean development of the Rhizoctonia solani isolate and the efficiency of the T. harzianum strain to seed germination success were determined by the water agar method. In the presence of T. harzianum and Rhizoctonia solani, bean development parameters were investigated by pot experiment. Physiological parameters (root and stem length, number of hair root, leaf surface area and number, wet and dry weights, etc.) were measured and then statistical analyzes were performed.

Trichoderma harzianum ID11C strain was determinated to tolerant against to high concentrations of heavy metals (100-400 mM) and salt (250 mM). It was observed that there were enzyme activities that promote plant growth. It was determined that T. harzianum strain had no negative effect on the germination success of the seeds compared to the control. In the pot experiment, when the control group and R. solani groups were compared with each other, it was determined that there was a significant difference between the results of the statistical analysis performed with the stem and main root length, the number of lateral root and the root lesion scale (Tukey test, p<0.05). The effects of R. solani or T. harzianum on bean development were not as good as the control group. However, ID11C and B227+ID11C groups were found to reduce pathogenic activity when compared with group B227.

It was determined that R. solani caused pathogenesis in the bean (Zulbiye), whereas T. harzianum strain decreased pathogenicity in all parameters, although it is not statistically significant. It was concluded that T. harzianum ID11C strain could be used as biocontrol and plant supporting agent against Rhizoctonia solani in bean farming.

Keywords

Trichoderma harzianum,Rhizoctonia solani,biocontrol,plant pathogen

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Şengül Alpay Karaoğlu ^*
0000-0003-1047-8350
Türkiye

Arif Bozdeveci
0000-0002-0729-9143
Türkiye

Müberra Pınarbaş Bu kişi benim
0000-0001-6064-0673
Türkiye

Gökhan Veyisoğlu Bu kişi benim
0000-0003-0224-3148
Türkiye

Cemre Tarakçı Bu kişi benim
0000-0003-0171-5369
Türkiye

Yayımlanma Tarihi

30 Aralık 2019

Gönderilme Tarihi

18 Nisan 2019

Kabul Tarihi

1 Temmuz 2019

Yayımlandığı Sayı

Yıl 2019 Cilt: 4 Sayı: 3

DOI

https://doi.org/10.35229/jaes.555230

IZ

https://izlik.org/JA34LX36SW

APA

Alpay Karaoğlu, Ş., Bozdeveci, A., Pınarbaş, M., Veyisoğlu, G., & Tarakçı, C. (2019). Fasulye gelişiminde, Rhizoctonia solani B227’nin biyotik stresine karşı Trichoderma harzianum ID11C’nin biyokontrol etkinliği. Journal of Anatolian Environmental and Animal Sciences, 4(3), 302-311. https://doi.org/10.35229/jaes.555230

JAES, 2016- 11.yıl

Fasulye gelişiminde, Rhizoctonia solani B227'nin biyotik stresine karşı Trichoderma harzianum ID11C'nin biyokontrol etkinliği

Öz

Anahtar Kelimeler

The biocontrol activity of Trichoderma harzianum ID11C against to the biotic stress of Rhizoctonia solani B227 in bean development

Abstract

Keywords

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

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