Sucul Bitki Bacopa monnieri (L.) Wettst.’nin Yaprak Eksplantlarından Çoklu Sürgün Rejenerasyonu Üzerine Zeatin Ön Uygulamasının Etkisi

Yıl 2019, , 161 - 165, 31.08.2019

Muhammet Doğan

https://doi.org/10.35229/jaes.557425

Öz

Bacopa monnieri
(L.) Wettst su ortamının iyileştirilmesinde ve geleneksel tıp sisteminde önemli
bir bitkidir. Bu çalışmada, B. monnieri’nin
doku kültürü teknikleri ile üretimi hedeflenmiştir. B. monneri’nin yaprak eksplantları farklı dozlarda Zeatin
(ZEA)’li solüsyonlarda (0,5-8,0 mg/L) bir saat bekletilmiş ve ardından bitki
büyüme düzenleyici içermeyen kültür ortamına aktarılmıştır. Yaprak
eksplantlarından ilk sürgün çıkışları 16.
günde 4 mg/L ZEA ön uygulamasında gözlenmiştir. Sürgün rejenerasyon frekansı
%16,66-88,89 arasında sıralanmıştır. En yüksek sürgün rejenerasyon frekansları
4 ve 8 mg/L ZEA ön uygulamasında elde edilmiştir. Genel olarak ZEA
konsantrasyonu arttıkça sürgün sayısı ve sürgün uzunluğu da artmıştır. Eksplant
başına maksimum sürgün sayısı (10,31 sürgün/eksplant) 4 mg/L ZEA ön
uygulamasında, en uzun sürgünler ise (2,02 cm) 8 mg/L ZEA ön uygulamasında
tespit edilmiştir. Rejenere sürgünler 0,25 mg/L indol-3-butirik asit (IBA)
içeren kültür ortamında köklendirildikten sonra dış koşullara başarıyla
alıştırılmıştır. Sonuç olarak, ZEA ön uygulamasının B. monnieri’nin in vitro
üretimindeki etkinliği kaydedilmiştir.  

Anahtar Kelimeler

Sucul bitki, doku kültürü, sürgün rejenerasyonu, yaprak eksplantı

Effect of Pre-treatment of Zeatin on Multiple Shoot Regeneration from Leaf Explants of Aquatic Plant Bacopa monnieri (L.) Wettst.

Öz

Bacopa
monnieri (L.) Wettst is an important plant in the remediation
of aquatic environment and in the traditional medicine system. In this study,
the propagation of B. monnieri by
tissue culture techniques was aimed. Leaf explants of B. monneri were kept in different doses of Zeatin (ZEA) solutions
(0.5-8.0 mg/L) for one hour and then transferred to culture medium without
plant growth regulator. The first shoot exits from the leaf explants were
observed at 16 days with 4 mg/L ZEA pre-treatment. The frequency of shoot
regeneration was between 16.66-88.89%. The highest shoot regeneration
frequencies were obtained at 4 and 8 mg/L ZEA pre-treatment. In general, the
number of shoots and shoot length increased as the ZEA concentrations
increased. The maximum number of shoots per explant (10.31 shoots/explant) was
determined in the 4 mg/L ZEA pre-treatment, and the longest shoot (2.02 cm) was
detected in the 8 mg/L ZEA pre-treatment. The regenerated shoots were
successfully acclimatized to the external conditions after rooting in culture
medium containing 0.25 mg/L of indole-3-butyric acid (IBA). In conclusion, the
efficacy of the ZEA pre-treatment for in
vitro propagation of B. monnieri
was recorded.

Anahtar Kelimeler

Aquatic plant, tissue culture, shoot regeneration, leaf explant

Kaynakça

• Altpeter, F., Springer, N.M., Bartley, L.E., Blechl, A.E., Brutnell, T.P., Citovsky, V., Conrad, L., Gelvin, S.B., Jackson, D., Kausch, A.P., Lemaux, P.G., Medford, J.I., Orozco-Cardenas, M., Tricoli, D., VanEck, J., Voytas, D.F., Walbot, V., Wang, K., Zhang, Z.J. & Stewart, C.N. (2016). Advancing crop transformation in the era of genome editing. Plant Cell 28, 1510-1520.
• Chone, R.M.S., Rocha, D.I., Monte-Bello, C.C., Pinheiro, H.P., Dornelas, M.C., Haddad, C.R.B. & Almeida, J.A.S. (2018). Brassinosteroid increases the cytokinin efficiency to induce direct somatic embryogenesis in leaf explants of Coffea arabica L. (Rubiaceae). Plant Cell Tissue and Organ Culture, 135, 63-71.
• Cirik, Ş., Cirik, S. & Conk-Dalay, M. (2011). Su Bitkileri II (İçsu Bitkilerinin Biyolojisi, Ekolojisi, Yetiştirme Teknikleri). Ege Üniversitesi, Su Ürünleri Fakültesi Yayınları, İzmir.
• Dias, M.I., Sousa, M.J., Alves, R.C. & Ferreira, C.F.R.I. (2016). Exploring plant tissue culture to improve the production of phenolic compounds: A review. Industrial Crops and Products, 82, 9-22.
• Dogan, M. (2019). Callus formation from full leaf and leaf parts of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne. Acta Biologica Turcica, 32, 78-83.
• Dogan, M. (2019). In vitro rapid propagation of an aquatic plant Pogostemon erectus (Dalzell) Kuntze. Anatolian Journal of Botany, 3, 1-6.
• Dogan, M. (2019). Multiple shoot regeneration via ındirect organogenesis from shoot tip and nodal meristem explants of Ceratophyllum demersum L. Journal of Animal and Plant Sciences, 29, 568-577.
• Doğan, M. (2018). In vitro shoot regeneration of Limnophila aromatica (Lamk.) Merr. from nodal and internodal explants. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 8, 77-84.
• Emsen, B. & Dogan, M. (2018). Evaluation of antioxidant activity of in vitro propagated medicinal Ceratophyllum demersum L. extracts. Acta Scientiarum Polonorum-Hortorum Cultus, 17, 23-33.
• Enyew, M. & Feyissa, T. (2019). In vitro shoot regeneration from leaf explants of Echinops kebericho: an endangered endemic medicinal plant. Plant Biosystems, 153, 199-204.
• Faria, D.V., Simao, M.J., Cipriano, R., Werner, E.T., Soares, T.C.B. Aoyama, E.M. & Lima-Gontijo, A.B.P. (2018). In vitro morphogenesis and micropropagation of Aechmea ramosa var. ramosa Mart. ex Schult. f. (Bromeliaceae) from leaf explants. In Vitro Cellular & Developmental Biology-Plant, 54, 530-536.
• Hussain K. & Nabeesa, S. (2012). Bioaccumulation pattern of mercury in Bacopa monnieri (L. ) Pennell. Journal of Stress Physiology & Biochemistry, 8, 10-21.
• Hussain, K., Abdussalam, A.K., Chandra P.R. & Salim, N. (2011). Heavy metal accumulation potential and medicinal property of Bacopa monnieri-a paradox. Journal of Stress Physiology & Biochemistry 7, 39-50.
• Jenks, M.A., Kane, M.E., Dennis, B. & McConnell, D.B. (2000). Shoot organogenesis from petiole explants in the aquatic plant Nymphoides indica. Plant Cell, Tissue and Organ Culture, 63, 1-8.
• Kim, D.H., Gopal, J. & Sivanesan, I. (2017). Nanomaterials in plant tissue culture: the disclosed and undisclosed. RSC Advances, 7, 36492-36505.
• Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497.
• Özel, Ç.A. (2018). Efficient in vitro plant regeneration from cultured leaf and petiole explants of Isatis constricta Davis. Acta Sci. Pol. Hortorum Cultus, 17, 49-55.
• Rai, P.K. (2009). Heavy metal phytoremediation from aquatic ecosystems with special reference to macrophytes. Critical Reviews in Environmental Science and Technology, 39, 697-753.
• Ribeiro de Souza, R., Duarte de Oliveira Paiva, P., Reis da Silva, R., Corrêa da Silva, D.P., Valquíria dos Reis, M. & Paiva, R. (2019). Morphogenetic potential of different sources of explants for efficient in vitro regeneration of Genipa sp. Plant Cell, Tissue and Organ Culture, 136, 153-160.
• Shahzad, A., Parveen, S. & Fatema, M. (2011). Development of a regeneration system via nodal segment culture in Veronica anagallis-aquatica L. - an amphibious medicinal plant. Journal of Plant Interactions, 6, 61-68.
• Singh, D., Tiwari, A. & Gupta, R. (2012). Phytoremediation of lead from wastewater using aquatic plants. Journal of Agricultural Science and Technology, 8, 1-11.
• Sivanesan, I. & Park, S.W. (2015). Optimizing factors affecting adventitious shoot regeneration, in vitro flowering and fruiting of Withania somnifera (L.) Dunal. Industrial Crops and Products, 76, 323-328.
• Stanly, C., Bhatt, A. & Keng, C.L. (2011). An efficient in vitro plantlet regeneration of Cryptocoryne wendtii and Cryptocoryne becketti through shoot tip culture. Acta Physiologiae Plantarum, 33, 619-624.