Bacillus subtilis ve Lactobacillus plantarum Probiyotik Bakterilerinin Bazı Balık Patojenleri Üzerine in vitro Antagonistik Etkisi

Yıl 2019, , 278 - 284, 31.08.2019

Ekrem Şanver Çelik , Sebahattin Ergün , Sevdan Yılmaz

https://doi.org/10.35229/jaes.592933

Cited By: 1

Öz

Destekleyen Kurum

Çanakkale Onsekiz Mart Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Proje Numarası

FBA-2016-779

Teşekkür

Bu çalışma Çanakkale Onsekiz Mart Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından FBA-2016-779 no’lu proje ile desteklenmiştir. Ayrıca proje süresince laboratuar çalışmalarında bizden yardımlarını esirgemeyen Samet ALKAN ve Çağatay BAYİZİT'e teşekkürlerimizi sunarız.

In vitro Antagonistic activity of Probiotic Bacteria Bacillus subtilis and Lactobacillus plantarum on Fish Pathogens

Öz

Bu çalışmada iki
potansiyel probiyotik bakteri Bacillus
subtilis ve Lactobacillus plantarum’
un bazı balık patojenlerine karşı antagonistik etkisi araştırılarak, su
ürünleri yetiştiriciliğinde kullanılabilirlikleri değerlendirilmiştir. Bu
amaçla Aeromonas sobria, Citrobacter sp., Lactococcus garvieae, Listonella
anguillarum, Edwardsiella tarda, ve
Yersinia ruckeri patojenleri üzerine
probiyotiklerin ürettikleri antibakteriyel maddelerin etkisi sıvı kültür
inhibisyon testi ile in vitro olarak
değerlendirilerek antagonistik etkinin varlığı araştırılmıştır. Sıvı kültür inhibisyon
testi iki probiyotik türünün üst fazının 36 saatlik kültür sonucunda bazı balık
patojenlerinin çoğalmasını inhibe edebildiğini göstermiştir. Test sonuçları B. subtilis'in A. sobria, E. tarda ve L. garviae'nin inhibisyonunda etkili
olduğunu göstermiştir. L. plantarum
probiyotiğinin A. sobria, L. garviae, L. anguillarum ve Y. ruckeri'nin
büyümesini engellediği tespit edilmiştir. İleriki çalışmalarda Bacillus subtilis ve Lactobacillus plantarum probiyotik
bakterilerinin balık yemlerine ilave edilerek besleme çalışmalarının
yapılmasına ve bu çalışmada test edilen patojenlere karşı balıkların direnç
kazanımlarının araştırılmasına ihtiyaç vardır.

Anahtar Kelimeler

Bacillus subtilis, Lactobacillus plantarum, Probiyotik, Balık Patojenleri

Proje Numarası

FBA-2016-779

Kaynakça

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