Anoxybacillus thermarum A4 Suşundaki Katalaz Aktivitesinin İncelenmesi ve Tam Hücre İmmobilizasyonu

Yıl 2019, , 581 - 588, 30.12.2019

Barbaros Dinçer , Murat Durmaz Ahmet Adıgüzel

https://doi.org/10.35229/jaes.647364

Öz

Yapılan bu çalışma ile Anoxybacillus thermarum A4 suşunun, katalaz enzimini üretebilme kapasitesi ve üretilen enzimin bazı kinetik verileri incelendi. Bununla birlikte A. thermarum A4 suşunun tam hücre immobilizasyonu agar ve agaroz ortamlarında gerçekleştirilerek, katalaz aktivitesi karakterize edildi. A4 suşunun yüksek katalaz aktivitesine sahip olduğu belirlendi. A4 suşu katalazının en yüksek aktivitesini pH 7,0 ve 50 ^oC’de gösterdiği belirlendi. A4 suşu özütü katalazının 17,5±2,2 mM K_m değerine ve 250 000±980 U mg protein^-1  V_maks değerine sahip olduğu belirlenirken, tam hücre immobilizasyonu sonrası K_m değeri 200±28 mM ve V_maks değeri  50.000±413 U g jel^-1 olarak belirlendi. A. thermarum A4 suşundan elde edilen katalazın Sodyum azid, Potasyum siyanür, Civa (II) klorür ve 3-amino-1,2,4-triazol gibi bilinen katalaz inhibitörleri ile inhibe olduğu belirlendi. Bu çalışma sonucunda, materyal olarak kullanılan A. thermarum A4 suşu yüksek bir katalaz aktivitesine sahip olmasından dolayı hidrojen peroksidin kullanıldığı endüstri alanları için bir potansiyel katalaz kaynak olabileceği öngörüldü.  

Anahtar Kelimeler

Katalaz, Hidrojen Peroksit Oksidoredüktaz, Termofilik Bakteri

Destekleyen Kurum

Recep Tayyip Erdoğan Üniversitesi

Investigation of Catalase Activity from Anoxybacillus thermarum A4 strain and Whole Cell Immobilization

Öz

In this study, the capacity of Anoxybacillus
thermarum A4 strain to produce the catalase enzyme and some kinetic data of the
produced enzyme were investigated. In addition, A. thermarum A4 strain was performed immobilization of its whole
cell in agar and agarose environments, and their catalase activities were
characterized. It was determined that A4 strain had high catalase activity. The
highest activity of A4 strain catalase was determined at pH 7.0 and 50 ^oC.
While catalase of A4 strain extract was determined to have 17.5 ± 2.2 mM Km and
250,000 ± 980 U mg protein^-1 Vmax, 
after whole cell immobilization , Km value was determined as 200 ± 28 mM
and Vmax was 50,000 ± 413 U g gel^-1. The catalase obtained from A.
thermarum A4 strain was inhibited by known catalase inhibitors such as Sodium
azide, Potassium cyanide, Mercury (II) Lys and 3-amino-1,2,4-triazole. As a
result of this study, it was envisaged that the A. thermarum A4 strain used as material could be a potential
catalase source for the industrial areas where hydrogen peroxide was used
because of its high catalase activity.

Anahtar Kelimeler

Catalase, Hydrogen Peroxide Oxidoreductase, Thermophilic Bacteria

Kaynakça

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