Effect of different genetic manipulations on the production of xylanase enzyme in Streptomyces strains

Yıl 2020, , 224 - 230, 30.06.2020

Fatma Esen Sarıgüllü Önalan

https://doi.org/10.35229/jaes.679536

Cited By: 1

Öz

Soil samples were collected from five different areas of University of Çukurova and 3 Streptomyces strains were isolated. The xylanase activity (at the end of the seventh day) of S40, S47 and S48 strains were found as 50,92U/mL, 49,51U/mL and 47,79U/mL respectively. After doing the protoplast fusion among these strains which have the highest xylanase activity, 9 strains which have different colony morphologies, were selected. The xylanase activity of (40-48) R2 and (47-48) R3 strains were increased in comparison with their parents, by 25,9% and 31,9% respectively. This study also indicated that the enzyme activity can be improved by protoplast fusion technique

Anahtar Kelimeler

Streptomyces, xylanase, , protoplast fusion

Proje Numarası

FEF2009D20

Streptomyces suşlarında farklı genetik manipulasyonların ksilinaz enzim üretimi üzerine etkisi

Öz

Çukurova Üniversitesi’nin 5 farklı bölgesinden alınan toprak örneklerinden 3 Streptomyces suşu izole edildi. S40, S47, S48 suşlarının 7 gün sonundaki ksilanaz aktivitesi sırasıyla 50,92 U/mL, 49,51 U/mL, 47,79 U/mL tespit edilmiştir. Yüksek ksilanaz aktivitesi gösteren bu suşlar arasında gerçekleştirilen protoplast füzyon çalışması sonucu farklı koloni morfolojsi gösteren 9 suş seçilmiştir. (40-48)R2 ve (47-48)R3 suşlarının ksilanaz aktivitesi ebeveyn suşlara göre sırasıyla %25,9 ve % 31,9 oranında arttığı tespit edilmiştir. Protoplast füzyon tekniğiyle enzim aktivitesinin geliştirilebileceği gösterilmiştir.

Anahtar Kelimeler

Streptomyces, ksilanaz, protoplast füzyonu

Destekleyen Kurum

Çukurova Üniversitesi

Proje Numarası

FEF2009D20

Teşekkür

Çukurova Üniversitesi BAP birimine katkılarından dolayı teşekkürler.

Kaynakça

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