Influence of Lead and Applied Singly and in Mixture with Chitosan on Tissue Accumulation of Clarias gariepinus
Abstract
Clarias gariepinus was exposed to 1 ppm Pb singly and in mixture with 75 ppm chitosan (CT) and the levels of Pb in gill, liver, spleen, kidney and muscle tissues were determined after 1, 7 and 15 days. Lead accumulation increased in gill tissue compared to control when exposed to Pb alone and in mixture on 7th day. A significant decrease, however, was observed in gill level on exposure to Pb+CT mixture on day 15. Exposure to Pb alone increased the level in liver significantly after 15 days compared to control fish. Exposure to Pb alone and to mixture had no effect on kidney accumulation. Pb accumulation increased in spleen tissue compared to control when exposed to Pb alone on 1st and 7th days, it decreased when exposed to Pb+CT mixture at these exposure periods. Muscle Pb accumulation was higher in fish exposed to Pb only as compared to controls and in fish exposed to Pb+CT mixture on 1st day. The studied lead concentrations caused alterations in the metal accumulation especially gill and liver tissues. Chitosan seemed to decrease the harmful effects of Pb on long term exposures.
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- Abbas, H.H.H., Mahmoud, H.M. & Miller, J.D. (2007). Vitamin C and Cadmium Toxicity in Fish Oreochromis niloticus. Online Journal of Veterinary Research 11, 54-74.
- Cicik, B. (2003). Bakır-Çinko Etkileşiminin Sazan (Cyprinus carpio L.)’nın Karaciğer, Solungaç ve Kas Dokularındaki Metal Birikimi Üzerine Etkileri. Ekoloji, 12(48), 32-36.
- Duran, S., Tunçsoy, M., Yeşilbudak, B., Ay, Ö., Cicik, B. & Erdem, C. (2015). Metal Accumulation in Various Tissues of Clarias Gariepinus Exposed to Copper, Zinc, Cadmium and Lead Singly and in Mıxture. Fresenius Environmental Bulletin. 24(12c), 4738-4742.
- Firidin, G. (2019). Effects of Lead and Its Selenium Mixtures on Biochemical Parameters of Oreochromis niloticus. Fresenius Environmental Bulletin. 28(1), 383-390.
- Gaber H.S. (2007). Impact of Certain Heavy Metals on the Gill and Liver of the Nile Tilapia Oreochromis niloticus, Egyptian Journal of Aquatic Biology and Fisheries, 11, 79–100.
- Garcia-Abiado, M.A., Mbahinzireki, G., Rinchard, J., Lee, K.J. & Dabrowski, K. (2004). Effect of Diets Containing Gossypol on Blood Parameters and Spleen Structure in Tilapia, Oreochromis sp., reared in a Recirculating system. Journal of Fish Diseases, 27(6), 359-368.
- Heath, A.G. (1995). Water Pollution and Fish Physiology. CRC press, Florida, USA, pp. 245.
- Hollis, L., Mcgeer, J.C., Mcdonald, D.G. & Wood, C.M. (1999). Cadmium Accumulation, Gill Cd Binding, Acclimation and Physiological Effects during Long Term Sublethal Cd Exposure in Rainbow Trout. Aquatic Toxicology, 46, 101–119.
- Krishnamurthy, K.V. & Harris, G.M. (1960). The Chemistry of the Metal Oxalato Complexes. Chemical Reviews, 1, 213-246.
- Kusemiju, V., Amoruwa, P. & Aderinola, J.A. (2012). Accumulation of Lead in the Tissues of Freshwater Catfish Clarias gariepinus Exposed to Static Nominal Concentrations of Lead Nitrate. Agriculture and Biology Journal of North America, 3(12), 510-515.