Molecular Characterization of Anisakis typica (Nematoda: Anisakidae) from the Mediterranean Sea Coasts of Turkey: First Mitochondrial rrnS Sequence Data

Yıl 2020, , 241 - 244, 30.06.2020

Gökmen Zafer Pekmezci , Ertan Emek Onuk

https://doi.org/10.35229/jaes.738822

Cited By: 3

Öz

There is no study about the molecular characterization of the mitochondrial rrnS gene of Anisakis typica from the Turkish Mediterranean coasts. Thus, molecular characterization of the rrnS gene of A. typica from Turkish waters is aimed for the first time in the present study. Ascaridoid marine nematodes were collected and morphologically identified by light microscope. Identified Anisakis type I larvae were randomly selected and molecularly analysed by Restriction Fragment Length Polymorphism (RFLP) for the internal transcribed spacer (ITS) region. Anisakis typica larvae identified by PCR-RFLP analyses were amplified and sequenced with both directions for the rrnS gene. The rrnS sequence of A. typica from the Mediterranean coasts of Turkey (MT395672) showed 99.80% identity (100% query coverage) with rrnS gene of A. typica (JX500052) from the Caribbean Sea, Atlantic Ocean. Between A. typica from the Mediterranean Sea (MT395672) isolate and the Caribbean Sea isolate differed by only one nucleotide (C‒T, at alignment position 437). Pairwise genetic distance between the rrnS sequence of A. typica herein and other A. typica from the Caribbean Sea was 0.002. Consequently, we determined the mitochondrial rrnS data of A. typica from the Mediterranean Sea in the present study for the first time. The valid genetic data (MT395672) reported here can be used to molecular identification of Anisakis species from the Mediterranean Sea and worldwide.

Anahtar Kelimeler

Anisakis typica, Mediterranean Sea, small subunit of rRNA (rrnS), molecular characterization

Türkiye'nin Akdeniz Kıyılarından Anisakis typica'nın (Nematoda: Anisakidae) Moleküler Karakterizasyonu: İlk Mitokondriyal rrnS Dizi Verileri

Öz

Anahtar Kelimeler

Anisakis typica, Akdeniz, küçük alt ünite rRNA (rrnS), moleküler karakterizasyon

Kaynakça

• Altschul, S.F., Gish, W., Miller, W., Myers, E.W. & Lipman, D.J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215, 403-410. doi: 10.1016/S0022-2836(05)80360-2. Berland, B. (1961). Nematodes from some Norwegian marine fishes. Sarsia, 2, 1-50.
• D'Amelio, S., Mathiopoulos, K. D., Santos, C. P., Pugachev, O. N., Webb, S. C., Picanço, M. & Paggi, L. (2000). Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: Ascaridoidea) defined by polymerase-chain-reaction-based restriction fragment length polymorphism. International Journal for Parasitology, 30(2), 223-226. doi: 10.1016/s0020-7519(99)00178-2.
• D'Amelio, S., Barros, N.B., Ingrosso, S., Fauquier, D.A., Russo, R. & Paggi L. (2007). Genetic characterization of members of the genus Contracaecum (Nematoda: Anisakidae) from fish-eating birds from west-central Florida, USA, with evidence of new species. Parasitology, 134, 1041-1051. doi: 10.1017/S003118200700251X.
• Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C., Thierer, T., Ashton, B., Meintjes, P. & Drummond, A. (2012). Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28(12), 1647-1649.
• Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018). MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology Evoluation, 35, 1547-1549. doi: 10.1093/molbev/msy096.
• Mattiucci, S., Cipriani, P., Levsen, A., Paoletti, M. & Nascetti, G. (2018). Molecular epidemiology of Anisakis and Anisakiasis: an ecological and evolutionary road map. Advances in Parasitology, 9, 93-263. doi: 10.1016/bs.apar.2017.12.001.
• Mattiucci, S. & Nascetti, G. (2008). Advances and trends in the molecular systematics of anisakid nematodes, with implications for their evolutionary ecology and host-parasite co-evolutionary processes. Advances in Parasitology, 66, 47-148. doi: 10.1016/S0065-308X(08)00202-9.
• Mattiucci, S., Cipriani, P., Webb, S. C., Paoletti, M., Marcer, F., Bellisario, B., Gibson, D. & Nascetti, G. (2014). Genetic and morphological approaches distinguish the three sibling species of the Anisakis simplex species complex, with a species designation as Anisakis berlandi n. sp. for A. simplex sp. C (Nematoda: Anisakidae). Journal of Parasitology, 100, 199-214. doi: 10.1645/12-120.1.
• Pekmezci, G.Z. (2019). Molecular characterization of Hysterothylacium aduncum (Nematoda: Raphidascarididae) larvae infecting Merlangius merlangus euxinus (Linnaeus, 1758) from the Turkish Black Sea coast based on mitochondrial small subunit ribosomal RNA gene analysis. Etlik Veteriner Mikrobiyoloji Dergisi, 30(1), 64-69. doi:10.35864/evmd.562427
• Pekmezci, G.Z., Onuk, E.E., Bolukbas, C.S., Yardimci, B., Gurler, A.T., Acici, M. & Umur, S. (2014). Molecular identification of Anisakis species (Nematoda: Anisakidae) from marine fishes collected in Turkish waters. Veterinary Parasitology, 201, 82-94. doi: 10.1016/j.vetpar.2014.01.005.
• Pekmezci, G. Z. & Yardimci, B. (2019). On the occurrence and molecular identification of Contracaecum larvae (Nematoda: Anisakidae) in Mugil cephalus from Turkish waters. Parasitology Research, 118(5), 1393-1402. doi: 10.1007/s00436-019-06278-x.
• Peter, A.J. & Maillard, C. (1988). Larves d’ascarides parasites de poisons en Mediterranee occidentale. Bulletin du Museum National d'histoire Naturelle, 10 (sect. A), 347-369.
• Zhu, X., Gasser, R.B., Podolska, M, & Chilton, N.B. (1998). Characterization of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences. International Journal for Parasitology, 28, 1911-1921. doi:10.1016/s0020-7519(98)00150-7