Molecular Characterization of Anisakis typica (Nematoda: Anisakidae) from the Mediterranean Sea Coasts of Turkey: First Mitochondrial rrnS Sequence Data
Abstract
There is no study about the molecular characterization of the mitochondrial rrnS gene of Anisakis typica from the Turkish Mediterranean coasts. Thus, molecular characterization of the rrnS gene of A. typica from Turkish waters is aimed for the first time in the present study. Ascaridoid marine nematodes were collected and morphologically identified by light microscope. Identified Anisakis type I larvae were randomly selected and molecularly analysed by Restriction Fragment Length Polymorphism (RFLP) for the internal transcribed spacer (ITS) region. Anisakis typica larvae identified by PCR-RFLP analyses were amplified and sequenced with both directions for the rrnS gene. The rrnS sequence of A. typica from the Mediterranean coasts of Turkey (MT395672) showed 99.80% identity (100% query coverage) with rrnS gene of A. typica (JX500052) from the Caribbean Sea, Atlantic Ocean. Between A. typica from the Mediterranean Sea (MT395672) isolate and the Caribbean Sea isolate differed by only one nucleotide (C‒T, at alignment position 437). Pairwise genetic distance between the rrnS sequence of A. typica herein and other A. typica from the Caribbean Sea was 0.002. Consequently, we determined the mitochondrial rrnS data of A. typica from the Mediterranean Sea in the present study for the first time. The valid genetic data (MT395672) reported here can be used to molecular identification of Anisakis species from the Mediterranean Sea and worldwide.
Keywords
Anisakis typica,Mediterranean Sea,small subunit of rRNA (rrnS),molecular characterization
Kaynakça
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