Cyfluthrin Pestisitinin Zebra Midyesi (Dreissena polymorpha) Üzerindeki Etkisinin Bazı Antioksidan Enzim Aktiviteleriyle Belirlenmesi

Yıl 2021, , 77 - 83, 31.03.2021

Osman Serdar

https://doi.org/10.35229/jaes.804479

Cited By: 5

Öz

Yapılan bu çalışmada, Dreissena polymorpha’da ticari insektisit olan Cyfluthrin (CFT)’nin bazı biyokimyasal yanıtları araştırılmıştır. CFT’nin D. polymorpha üzerindeki 96 saatlik LC50 değeri 553,22 ± 27,3 µg/L olarak hesaplanmıştır. D. polymorpha, CFT’nin subletal konsantrasyonlar (LC50 değerinin 1/20, 1/10 ve 1/5)’ına 24 ve 96 saat süre ile maruz bırakılmıştır. D. polymorpha bireylerinde superoksit dismustaz (SOD), katalaz (CAT) ve Glutatyon peroksidaz (GPx) enzim aktiviteleri belirlenmiştir. CFT’ye maruz bırakılan D. polymorpha’da, SOD aktivitesi kontrole kıyasla artmıştır. CAT ve GPx aktivitelerinin kontrole kıyasla inhibe olduğu belirlenmiştir.
Sonuç olarak, CFT'ye maruz kalan D. polymorpha bireylerinde oksidatif hasarda artışa olduğu bulunmuştur.

Anahtar Kelimeler

Dreissena polymorpha, Cyfluthrin, Glutatyon peroksidaz, Superoksit dismustaz

Destekleyen Kurum

Munzur Üniversitesi Bilimsel Araştırmalar Proje Birimi

Proje Numarası

PPMUB019-17.

Determination of the Effect of Cyfluthrin Pesticide on Zebra Mussel (Dreissena polymorpha) by Some Antioxidant Enzyme Activities

Öz

In this study, some biochemical responses of Cyfluthrin (CFT), a commercial insecticide in Dreissena polymorpha, were investigated. The 96 hour LC50 value of CFT on D. polymorpha was calculated as 553.22 ± 27.3 µg / L. D. polymorpha was exposed to subletal concentrations (1/20, 1/10 and 1/5 of LC50 value) of CFT for 24 and 96 hours. The enzyme activities of superoxide dismustase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were determined in D. polymorpha individuals. In D. polymorpha exposed to CFT, SOD activity increased compared to control. It was determined that CAT and GPx activities were inhibited compared to the control.
As a result, D. polymorpha individuals exposed to CFT were found to cause increased oxidative damage.

Anahtar Kelimeler

Dreissena polymorpha, Catalase, Cyfluthrin, Glutathione peroxidase, Superoxide dismustase

Proje Numarası

PPMUB019-17.

Kaynakça

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