Effects of essential amino acids on development and synthesised protein amount of male larvae of Pimpla turionellae L. (Hymenoptera: Ichneumonidae).

Yıl 2021, , 92 - 98, 31.03.2021

Mehmet Sulanç

https://doi.org/10.35229/jaes.830843

Cited By: 1

Öz

Effects of different essential amino acids ratios on the rate of development and the percentage of synthesised protein amount on the male larvae of endoparasitoid hymenopterous, Pimpla turionellae L. were investigated by using chemically defined synthetic diets under aseptic conditions. It was shown that the amino acids isoleucine, leucine and methionine had a qualitatively and quantitatively critical effect on the developmental rate and protein synthesis increased after increasing the rate of isoleucine and leucine by 50% and decreasing the rate of methionine by 50%, however decreased both the development date and protein synthesis.
It was decided that arginine, histidine, lysine, phenylalanine, threonine, tryptophan and valine had no critical qualitative or quantitative effect and that their rates in the control diet should be assumed optimal.

Anahtar Kelimeler

Pimpla turionellae, Essential amino acids, Development, Synthesised protein

Temel Amino Asitlerin Erkek Pimpla turionellae L. (Hymenoptera: Ichneumonidae) Larvalarının Gelişmesine ve Sentezledikleri Protein miktarına Etkileri

Öz

Temel amino asitlerin farklı oranlarının bir endoparazitoid hymenopter türü olan Pimpla turionellae L. erkek larvalarının gelişme hızı ve sentezledikleri protein miktarı üzerine etkileri, larvaların kimyasal yapısı bilinen sentetik besinler kullanılarak aseptik koşullarda beslemek suretiyle incelenmiştir. İzolösin, lösin ve metiyoninin larvaların gelişim hızı ve protein sentezi üzerinde kalitatif ve kantitatif olarak kritik bir etkiye sahip olduğu, izolösin ve lösinin besindeki oranının %50 arttırılması, metiyoninin %50 azaltılması ile hem gelişme hızı hem de protein sentezi üzerinde değişik etkiler yaptığı gösterilmiştir.
Arjinin, histidin, lizin, fenilalanin, treonin, triptofan ve valinin kalitatif ve kantitatif olarak kritik etkisi olmadığına ve kontrol besindeki oranlarının optimal kabul edilmesi gerektiğine karar verildi.

Anahtar Kelimeler

Pimpla turionellae, temel amino asitler, gelişme, protein sentezi

Kaynakça

• Arthur, A.P., Hegdekar, B.M. & Batsch, W.W. (1972). A chemically defined synthetic medium that induces oviposition in the parasite Itoplectis conquisitor (Hymenoptera : Ichneumonidea) . Canada Entomology 104, 1251-1258.
• Assar, A.A., Abo El-Mahasen, M.M., Khalil, M.E. & Mahmoud, S.H. (2010). Biochemical effects of some insect growth regulators on the house fly, Musca domestica (Diptera:Muscidae). Egyptian Academic Journal of Biological Sciences 2, 33-44.
• Chang, C.L. (2004). Effect of amino acids on larvae and adults of Ceratitis capitata (Diptera: Tephritidae).Annals of the Entomological Society of America 97, 529-535.
• Chen, C.P. (1985). Amino Acid and Protein Metabolism. In: Kerkut, G. A., Gilbert, L. I. (Eds.). Comprehensive Insect Physiology Biochemistry and Pharmacology. Pergamon Press, Oxford, 177-199. Çelik, S. (1984). Pimpla turionellae L (Hymenoptera:Ichneumonidae)’nın kalitatif ve kantitatif Amino asit ihtiyaçları. Doğa Bilim Dergisi A2 8(3), 321-329.
• Dadd, R.H. (1985). Nutrition: Organisms. In Comprehensive Insect Physiology, Biochemistry and Pharmacology. Ed. by Kerkut, G.A. and Gilbert, L.I., 8, 313-390. Pergamon Press, Oxford.
• Davis, G.R.F. (1961a). Sulfur-containing amino acids in the nutrition of the saw-toothed grain beetle, Oryzaephilus surinamensis (L.) (Coleoptera:Silvanidae). Journal Nutrition 75, 275-279.
• Davis, G.R.F. (1972). Quantitative requirements of the saw-toothed grain beetle, Oryzaephilus surinamensis for dietary glicine and L-threonine.Journal of Insect Physiology 18, 1287-1294.
• Davis, G.R.F. (1973). Quantitative requirements of the saw-toothed grain beetle, Oryzaephilus surinamensis for dietary L-isoleucine and L- valine. Journal of Insect Physiology 19, 1657- 1661.
• Emre, İ. (1988). Meridik bir besinin Pimpla turionellae L (Hymenoptera:Ichneumonidae) ergin dişilerinin yumurta verimine etkisi. Doğa Turk Biyoloji Dergisi 12(2), 101-105.
• Emre, İ. & Yazgan, Ş. (1990). Besin bileşenlerinin Pimpla turionellae L (Hymenoptera: Ichneumonidae)‘nın üremesi üzerine etkileri. Doğa Turk Journal of Biology 14, 96-104.
• Fısher, R.C. & Ganesalıngam, V.K. (1970). Changes in the composition of host hemolymph after attack by an insect parasitoid. Nature, London, 227,191-192.
• Plummer, D.T. (1971). An Introduction of Practical Biochemistry, McGraww-Hill Book Comp., pp 369.
• Richards, E.H. & Edwards, J.P. (1999). Parasitism of Lacanobia oleracea (Lepidoptera, Noctuidae) by the ectoparasitoid wasp Eulophus pennicornis, results in the appearance of a 27 kDa parasitism-specific protein in host plasma. Insect Biochemistry & molecularBiology 29, 557-569.
• Roe, H.J., Batley, J.M., Gray, R.R. & Robinson, J.N. (1961).Complete removal of glycogen from tissues by extraction with cold trichloroasetic acid solution. Journal of Biological Chemistry 236, 1224-1246.
• Sulanç, M., Emre, İ. & Yazgan, Ş. (1992). İnorganik tuzların erkek Pimpla turionellae L.(Hymenoptera: Ichneumonidae) larvalarının gelişmesine ve sentezledikleri protein miktarına kalitatif ve kantitatif etkileri. Doğa Turk Journal of Zoology 16, 92-100.
• Sulanç, M. & Yazgan, Ş. (1990). Aspartik asit, Glutamik asit ve amidlerinin (Asparajin,Glutamin) Pimpla turionellae L (Hymenoptera: Ichneumonidae) larvalarının gelişme ve sentezlenen protein miktarına etkileri. Ç.Ü.Fen ve Mühendislik Bilimleri Dergisi 4(2), 97-107.
• Sulanç, M. & Emre, İ. (2000). Effects of B group vitamins and choline chloride on the development and protein synthesis in the male larvae of Pimpla turionellae L (Hym., Ichneumonidae). Journal of Applied Entomology 124,151-153.
• Sokal, R.R. & Rohlf, F.J. (1995). Biometry: ThePrinciplesandPractice of Statistics in Biological Research, 3rdedition. W.H. FreemanandCo., New York, s. 887.
• Thompson, S.N. (1976a).The amino acid requirements for larval development of the hymenopterous parasitoid Exeristes roborator Fabricius (Hymenoptera:Ichneumonidae). Comparative Biochemistry and Physiology 53A, 211-213.
• Thompson, S.N. (1976b). Effects of dietary amino acid level and nutritional balance on larval survival and development of the parasite Exeristes roborator. Annals of the Entomological Society of America 69, 835 -838.
• Thompson, S.N. (1986). Nutrition and in vitro culture of insect parasitoids. Annual Review of Entomology 31,197-219.
• Thompson, S.N. & Lee, R.W.K. (1994). Glucose metabolism in an insect, Manduca sexta, and effects of parasitism. Biochimica et Biophysica Acta 1200, 322-330.
• Yanıkoğlu, A. (1985). Bazı karbonhidratların Pimpla turionellae L (Hymenoptera: Ichneumonidae)’nin glikojen sentezine etkileri.C.Ü.Fen-Edebiyat Fakültesi Fen Bilimleri Dergisi 3, 205-210.
• Yazgan, Ş. (1972). A Chemically Defined Synthetic Diet and Nutritional Requirement of the endoparasitoid Itoplectis conquisitor (Hymenoptera). Journal of Insect Physiology 18, 2123-2141.
• Yazgan, Ş. (1981). A meridic diet and quantitative effect of tween 80, fatty acid mixtures and İnorganic salts on development and survival of the endoparasitoid Pimpla turionellae L., Zeitschrift für angewandte. Entomologie 91, 433-441.
• Yazgan, Ş. (1982). Effects of dietary fatty acids on development and survival of Itoplectis conquisitor (Hymenoptera:Ichneumonidae). Comminications de la Faculte des Sciences de L’Universite d’Ankara,Serie C 26,1-8.