Gebe Sığırlara İnaktif Escherichia coli Aşısı Uygulamasının Kolostrum ve Buzağı Kan Serumlarındaki IgG Seviyelerine Etkisinin Araştırılması

Yıl 2021, , 318 - 324, 28.09.2021

Timur Gülhan , Sinan Sancak

https://doi.org/10.35229/jaes.886678

Öz

Bu çalışmada, gebe sığırlara uygulanan inaktif E. coli aşısının kolostrum ve buzağı kan serumlarındaki immunoglobulin (Ig) G seviyelerine olan etkisinin araştırılması amaçlandı. Bu amaçla, gebeliklerinin farklı dönemlerindeki sığırlara (son 60 ve 30 gün) ticari bir inaktif E. coli aşısı uygulandı. Aşılanan sığırların kolostrumu ve bu kolostrumlarla beslenen buzağıların kan serumlarındaki IgG seviyeleri ELISA ile incelendi. Aşılama grupları ve kontrol grubu arasında IgG seviyelerindeki farklılıklar ölçülerek, gruplar arasındaki IgG değerleri karşılaştırıldı. Kolostrum örneklerine ait veriler incelendiğinde, kontrol grubu ile tek doz ve iki doz aşılanan hayvanlardan elde edilen sonuçlar arasındaki farkın önemli (p<0,001) olduğu belirlendi. Benzer şekilde buzağı serumlarına ait veriler değerlendirildiğinde; tek doz ve iki doz aşı yapılanan sığırların buzağılarında ölçülen kan serumu değerlerinde kontrol grubuna göre önemli oranda artış tespit edildi (p<0,001). Diğer yandan kontrol grubundaki 7 buzağının tamamında (%100), tek doz aşılanan sığırlara ait 7 buzağının 5’inde (%71,4) pasif transfer yetersizliği (PTY) görülürken, iki doz aşılanan 7 buzağının hiçbirinde PTY belirlenemedi. Böylece aşılanan hayvanlarda PTY oranlarında önemli oranda düşüş sağlanmış oldu. Sonuç olarak gebe sığırlara uygulanan inaktif E. coli aşısı ile oluşturulan immun yanıtın pasif olarak buzağılara transfer edildiği, gebe sığırların gebeliklerinin son 60 ve 30. gününde iki doz aşılamanın, tek doz aşılamaya göre daha etkili olduğu kanaatine varıldı.

Anahtar Kelimeler

Blood sera, Bovine, Colostrums, ELISA, Inactive E. coli vaccine, IgG

Destekleyen Kurum

Ondokuz Mayıs Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı

Proje Numarası

PYO.VET.1904.20.004

Teşekkür

Bu çalışmaya PYO.VET.1904.20.004 nolu proje kapsamında maddi destek sağlayan Ondokuz Mayıs Üniversitesi Bilimsel Araştırma Projeleri Başkanlığına teşekkür ederiz

Investigation of Effect of Inactive Escherichia coli Vaccination on Pregnant Cattle IgG Levels in Colostrums and Calf Blood Serums

Öz

In this study, it was aimed to investigate the effect of inactive E. coli vaccine administered to pregnant cattle on immunoglobulin (Ig) G levels in colostrums and calf blood serum. For this purpose, a commercial inactive E. coli vaccine was administered to cattle at different stages of their pregnancy (last 60 and 30 days). The colostrums of vaccinated cattle and IgG levels in blood serum of calves fed with these colostrums were analyzed by ELISA. Differences in IgG levels between vaccination groups and control groups were measured and IgG values between groups were compared. When the data of the colostrum samples were examined, it was determined that the difference between the results obtained from the control group and the animals that were vaccinated with one dose and two doses was significant (p <0.001). Similarly, when the data of calf sera are evaluated; There was a significant increase in blood serum values measured in calves of cattle receiving single dose and two doses of vaccine compared to the control group (p <0.001). On the other hand, passive transfer failure (PTF) was observed in all 7 calves (100%) in the control group and in 5 (71.4%) of the 7 calves that were vaccinated with a single dose, while PTF could not be determined in any of the 7 calves that were vaccinated with two doses. Thus, a significant decrease in PTF rates was achieved in vaccinated animals. As a result, it was concluded that the immune response generated by the inactive E. coli vaccine administered to pregnant cattle was transferred to calves passively, and that two doses of vaccination on the last 60 and 30 days of pregnancy of pregnant cattle were more effective than single dose vaccination.

Anahtar Kelimeler

ELISA, İnaktif E. coli aşısı, IgG, Kan serumu, Kolostrum, Sığır

Proje Numarası

PYO.VET.1904.20.004

Kaynakça

• Acres, S.D., Isaacson, R.E., Babiuk, L.A. & Kapitany, R.A. (1979). Immunization of calves against enterotoxigenic colibacillosis by vaccinating dams with purified K99 antigen and whole cell bacterins. Infection and Immunity, 25(1), 121-126.
• Al-Alo KZK., Nikbakht Brujeni, G., Lotfollahzadeh, S., Moosakhani, F. & Gharabaghi, A. (2018). Correlation between neonatal calf diarrhea and the level of maternally derived antibodies. Iranian Journal of Veterinary Research, 19(1), 3-8.
• Avila, F.A., Paulillo, A.C., Schocken-Iturrino, R.P., Lucas, F.A., Orgaz, A. & Quintana, J.L. (1995). A comparative study of the efficiency of a pro-biotic and the anti-K99 and anti-A14 vaccines in the control of diarrhea in calves in Brazil. Revue d'élevage et de médecine vétérinaire des pays tropicaux. 48(3), 239-243.
• Collins, N.F., Halbur, T., Schwenck, W.H., Hoogeveen, P., Pierce, R.L., Behan, R.W. & Pankratz, D. (1988). Duration of immunity and efficacy of an oil emulsion Escherichia coli bacterin in cattle. American Journal of Veterinary Research, 49(5), 674-677.
• Contrepois, M., Girardeau, J.P., Dubourguier, H.C., Gouet, P. & Levieux, D. (1978). Specific protection by colostrum from cows vaccinated with the K99 antigen in newborn calves experimentally infected with E. coli Ent+ K99+. Annals of Veterinary Research, 9(2), 385-388.
• Crouch, C.F., Oliver, S. & Francis, M.J. (2001). Serological. colostral and milk responses of cows vaccinated with a single dose of a combined vaccine against Rotavirus, Coronavirus and Escherichia coli F5 (K99). Veterinary Record, 149, 105-108.
• da Silva, É.B.R., da Silva, W.C., de Sousa, E.D.V., da Cruz Gato, A.P., Araújo, L.J.S., de Moraes Sales, R., do Couto, T.M. & da Silva, A.A. (2019). Major diseases affecting neonatal calves. Research Society and Development, 8(8), e04881173. DOI: http://dx.doi.org/10.33448/rsd-v8i8.1173.
• Deluyker, H.A., Rossitto, P., Van Oye, S.N. & Cullor, J.S. (2004). Efficacy of an Escherichia coli J-5 mutant strain bacterin in the protection of calves from endotoxin disease caused by subcutaneous challenge with endotoxins from Escherichia coli. Vaccine, 23, 709-717.
• Dezfoulie, M.R.M., Lotfollahzadeh, S., Heidari, M. & Boroujeni, G.N. (2019). Clinical and paraclinical changes in experimental colisepticemia in neonatal calves. Journal of Istanbul Veterinary Science, 3(2), 49-56.
• Dubreuil, J.D., Isaacson, R.E & Schifferli, D.M. (2016). Animal enterotoxigenic Escherichia coli. EcoSal Plus. 7(1), doi:10.1128/ecosalplus.ESP-0006.
• Eichhorn, W., Bachmann, P.A., Baljer, G., Plank, P. & Schneider, P. (1983). Vaccination of cows with a combined rotavirus/enterotoxigenic E. coli K99 vaccine to protect newborn calves against diarrhoea. Developments in Biological Standardization, 53, 237-43.
• Figueiredo, H.C.P., Lage, A.P., Pereira Júnior, F.N. & Leite, R.C. (2004). Passive immunity in cattle against enterotoxigenic Escherichia coli: serologic evaluation of a bacterin containing K99 and F41 fimbriae in colostrum of vaccinated females and calf serum. Brazilian Journal of Veterinary and Animal Science, 56(4), 425-432.
• IBM Corp, (2011). IBM SPSS Statistics for windows, Version 20.0. IBM Corporation, Armonk, NY.
• Kohara, J., Hirai, T., Mori, K., Ishizaki, H. & Tsunemitsu, H. (1997). Enhancement of passiye immunity with maternal vaccine against newbom caif diarrhea. Journal of Veterinary Medical Science, 59, 1023-1025.
• Krogh, H.V. (1983). Infection with enterotoxigenic Escherichia coli in calves and protection of the calves by vaccination of the dams. Annals of Veterinary Research, 14(4), 522-525.
• Loucks, M.E., Morrıll, J.L. & Dayton, A.D. (1985). Effect of prepartum vaccination with K99 Escherichia coli vaccine on maternal and calf blood antibody concentration and calf health. Journal of Dairy Science, 68: 1841-1847.
• McGee, M. & Earley, B. (2019). Review: passive immunity in beef-suckler calves. Animal, 13(4): 810-825,
• Mohammed, S.A.E.M., Marouf, S.A.E.M., Erfana, A.M., El-Jakee, J.K.A., Hessain, A.M., Dawoud, T.M. & Moussa, I.M. (2019). Risk factors associated with E. coli causing neonatal calf diarrhea. Saudi Journal of Biological Sciences, 26, 1084-1088.
• Myers, L.L. (1976). Vaccination of cows with an Escherichia coli bacterin for the prevention of naturally occurring diarrheal disease in their calves. American Journal of Veterinary Research, 37(7), 831-834.
• Nagy, B. (1980). Vaccination of cows with a K99 extract to protect newborn calves against experimental enterotoxic colibacillosis. Infection and Immunity, 27(1): 21-24.
• Picco, N.Y., Alustiza, F.E., Bellingeri, R.V., Grosso, M.C., Motta, C.E., Larriestra, A.J., Vissio, C., Tiranti, K.I., Terzolo, H.R., Moreira, A.R. &Vivas, A,B. (2015). Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina. Revista Argentina de Microbiología, 47(2), 95-102.
• Selim, S.A., Cullor, J.S., Smith, B.P., Blanchardz, P., Farver, T.B., Hoffmant, R., Dilling, G., Da Rodent, L. & Wilgenburg, B. (1995). The effect of Escherichia coli J5 and modified live Salmonella dublin vaccines in artificially reared neonatal calves. Vaccine, 13(4), 381-390.
• Sherman, D.M., Acres, S.D., Sadowski, P.L., Springer, J.A., Bray, B., Raybould, T.J.G. & Muscoplat, C,C. (1983). Protection of calves against fatal enteric colibacillosis by orally administered Escherichia coli K99-specific monoclonal antibody. Infection and Immunity, 42(2), 653-658.
• Snodgrass DR, Nagy LK, Sherwood D, Campbell I. (1982). Passive immunity in calf diarrhea: Vaccination with K99 antigen of enterotoxigenic Escherichia coli and rotavirus. Infection and Immunity, 37(2), 586-591.
• Snodgrass, D.R. (1983). Development of calf diarrhoea vaccines. Annals of Veterinary Research, 14(4), 519-521. Snodgrass, D.R. (1986). Evaluation of a combined rotavirus and enterotoxigenic Escherichia coli vaccine in cattle. Veterinary Record, 119(2), 39-42.
• Stĕpánek, J., Salajka, E., Zuffa, A., Mensík, J. & Franz, J. (1987). A new polyvalent vaccine against enteral infections in newborn calves. Veterinarni Medicina, 32(2), 65-80.
• Valente, C., Fruganti, G., Tesei, B., Ciorba, A., Cardaras, P., Floris, A. & Bordoni, E. (1988). Vaccination of pregnant cows with K99 antigen of enterotoxigenic Escherichia coli and protection by colostrum in newborn calves. Comparative Immunology, Microbiology and Infectious Diseases, 11(3/4), 189-198.
• Yano, T., Garcia, M., Leite, D.S., Pestana-de-Castro, A.F. & Shenk, M.A. (1995). Determination of the efficiency of K99-F41 fimbrial antigen vaccine in newborn calves. Brazilian Journal of Medical and Biological Research, 28(6), 651-654.
• Waltner-Toews, D., Martin, S.W., Meek, A.H., MeMillan, I. & Croueh, C.F. (1985). A field trial to evaluate the efficacy of a combined rotavirus-coronavirus/Escherichia coli vaccine in dairy cattle. Canadian Journal of Comparative Medicine, 49, 1-9.
• Wieda, J., Bengelsdorff, H.J., Bernhardt, D. & Hungerer, K.D. (1987). Antibody levels in milk of vaccinated and unvaccinated cows against organisms of neonatal diarrhoea. Journal of Veterinary Medicine Series B, 34, 495-503.