Comparison to Toxic Effects of Copper Oxide Nanoparticles and Copper Sulphate on Some Serum Parameters and Enzyme Activities of Oreochromis niloticus
Abstract
Today, human effects due to industrial development and population increase caused water ecosystems to be polluted by various pollutant such as heavy metals. Serum biochemical parameters are widely used in blood analysis and these parameters are suitable indicators for monitoring physiological changes in fish. Hence, the aim of present study to the effects of waterborne copper on serum glucose, total protein, albumin and triglyceride levels and serum cholinesterase (ChE), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkalane phosphatase (ALP) and lactate dehydrogenase (LDH) activities of O. niloticus were determined after exposing the fish to 10, 50 and 100 µg/L copper applied as CuO nanoparticles (CuO NPs) and CuSO4 over 1, 7 and 15 days.
No mortality was observed during the experiments. Serum glucose and albumin levels increased while serum total protein, cholesterol and triglyceride levels decreased compared to control at the end of the 15th day. There was also a decrease in serum ChE activity whereas serum AST, ALT, ALP and LDH activities increased. Overall, CuSO4 and CuO NPs had similar effects in serum parameters of O. niloticus.
Keywords
O. niloticus , Copper oxide nanoparticles , copper sulphate , serum parameters
Kaynakça
- Abdel-Daim, M.M., Eissa, I.A., Abdeen, A., Abdel-Latif, H.M., Ismail, M., Dawood, M.A. & Hassan, A.M. (2019). Lycopene and resveratrol ameliorate zinc oxide nanoparticles-induced oxidative stress in Nile tilapia, Oreochromis niloticus. Environ. Toxicol. Pharmacol, 69:44-50.
- Abdel-Latif, H.M.R., Dawood, M.A.O., Mahmoud, S.F., Shukry, M., Noreldin, A.E., Ghetas, H.A. & Khallaf. M.A. (2021). Copper Oxide Nanoparticles Alter Serum Biochemical Indices, Induce Histopathological Alterations, and Modulate Transcription of Cytokines, HSP70, and Oxidative Stress Genes in Oreochromis niloticus. Animals, 11(3):652.
- Abdel-Khalek, A.A., Kadry, M.A.M., Badran, S.R. & Marie, M.S. (2015). Comparative Toxicity of Copper Oxide Bulk and Nano Particles in Nile Tilapia; Oreochromis niloticus: Biochemical and Oxidative Stress. J Basic Appl Biol, 72:43-57.
- Bhatt, I. & Tripathi, B.N. (2011). Interaction of Engineered Nanoparticles with Various Components of the Environment and Possible Strategies for Their Risk Assessment. Chemosphere 82(3):308-317.
- Brown, J.A. (1993). Endocrine Responses to Environmental Pollutants. In: Rankin, J.C., Jensen, F.B. (eds.) Fish Ecophysiology. Chapman and Hall, London, UK, s. 276-296.
- Buffet, P.E., Tankoua, O.F., Pan, J.F., Berhanu, D., Herrenknecht, C., Poirier, L., Amiard-Triquet, C., Amiard, J.C., Bérard, J.B., Risso, C., Guibbolini, M., Roméo, M., Reip, P., Valsami-Jones, E. & Mouneyrac, C. (2011). Behavioural and Biochemical Responses of Two Marine Invertebrates Scrobicularia plana and Hediste diversicolor to Copper Oxide Nanoparticles. Chemosphere 84:166-174.
- Bundschuh, M., Filser, J., Lüderwald, S., McKee, M.S., Metreveli, G., Schaumann, G.E., Schulz, R. & Wagner, S. (2018). Nanoparticles in the environment: where do we come from, where do we go to? Environ Sci Eur, 30(1):6.
- Chen, C.Y., Wooster, G.A. & Bowser, P.R. (2004). Comparative Blood Chemistry and Histopathology of Tilapia Infected with Vibrio vulnificus or Streptococcus iniae or Exposed to Carbon Tetrachloride, Gentamicin or Copper Sulphate. Aquac, 239:421-443.
- Cicik, B. & Engin, K. (2005). The Effects of Cadmium on Levels of Glucose in Serum and Glycogen Reserves in the Liver and Muscle Tissues of Cyprinus carpio (L., 1758). Turk J Vet Anim Sci, 29:113-117.
- De, T.K., De, M., Das, S., Ray, R. & Ghosh, P.B. (2010). Level of Heavy Metals in Some Edible Marine Fishes of Mangrove Dominated Tropical Estuarine Areas of Hooghly River, North East Coast of Bay of Bengal, India. Bull Environ Contam Toxicol, 85:385-390.