Escherichia coli Strains Isolated from Cats and Dogs with Urinary Tract Infections

Yıl 2018, Cilt: 3 Sayı: 3, 131 - 136, 18.12.2018

Arzu Funda Bağcıgil Banu Dokuzeylül Hüban Göçmen Ebru Yalçın Serkan İkiz Naciye Yakut Özgür Seyyal Ak

https://doi.org/10.35229/jaes.431221

Cited By: 3

Öz

The aim of this
study is to perform phylogenetic grouping, to detect the virulence associated
genes in Escherichia coli strains
recovered from the urinary tract infections of dogs and cats, and to determine
the antibiotic susceptibility profiles, as well. The urine samples from the
animals showing clinical signs of urinary tract infection examined for the
occurrence of E. coli. Phylogenetic
classification of the isolates was performed according to the existence of chuA, yjaA and TSPE4.C2 genes. The isolates were also examined for the
presence of uropathogenecity-associated genes, such as, fimH, sfa1, iut, fyu, hly,
cnf-1, papG and for the diversity of
the papG allelles. Finally, the
antimicrobial susceptibility profiles were examined. E. coli was isolated from 11 (6.08%) of 181 samples. Two isolates
belonged to group A-2, while the remaining isolates belonged to group B-2. hylA was detected in 2 (25%); sfa1, cnf1, papG and papGIII were detected in 3 (37.5%); iutA was detected in 4 (50%);  fyuA
was detected in 6 (75%); and fimH was
detected in all of the isolates. Most of the isolates were resistant to
ampicillin while one isolate also showed multidrug resistance. The isolation
rate of E. coli was low in this
study, however, we had a brief data about the phylo-groups and virulence
factors of canine and feline uropathogenic strains. 

Anahtar Kelimeler

Antibiotic resistance, Cats, Dogs, Phylo-typing, Uropathogenic Escherichia coli

Kaynakça

• Reference1. Babacan O., Akan M., İzgür M., (2011). Kedi ve köpeklerin ürogenital sistem infeksiyonlarından izole edilen Escherichia coli suşlarının antibiyotik duyarlılıklarının belirlenmesi. Veteriner Hekimler Derneği Dergisi. 82, 43-48.Reference 2. Çetin C., Şentürk S., Kocabıyık A.L., Temizel M., Özel E., (2003). Bacteriological examination of urine samples from dogs with symptoms of urinary tract infection. The Turkish Journal of Veterinary and Animal Sciences, 27, 1225-1229. Reference 3. Chang S.K., Lo D.Y., Wei H.W., Kuo H.C., (2015). Antimicrobal resistance of E. coli isolates from canine urinary tract infections. Journal of Veterinary Medical Sciences, 77, 59-65. Reference 4. Chen Y.M.M., Wright P.J., Lee C.S., Browning G.F., (2003). Uropathogenic virulence factors in isolates of Escherichia coli from clinical cases of canine pyometra and feces of healthy bitches. Veterinary Microbiology, 94, 57-69. Reference 5. Clermont O., Bonarcorsi S., Bingen E., (2000). Rapid and simple determination of the Escherichia coli phylogenetic group. Applied and Environmental Microbiology, 66, 4555-4558. Reference 6. Clermont O., Christenson J.K., Denamur E., Gordon D.V., (2013). The Clermont Escherichia coli phylo-typing method revisted: improvemet of specificity and detection of new phylo-groups. Environmental Microbiology Reports, 5, 58-65. Reference 7. Clinical and Laboratory Standards Institute (CLSI) (2013). M100-S23 Performance Standards for antimicrobial susceptibility testing: 23rd international supplement CLSI 2013, Wayne, Pennsylvania; 2013. Reference 8. Drazenovich N., Ling G.V., Foley J. (2004). Molecular investigation of Escherichia coli strains associated with apparently persistent urinary tract infection in dogs. Journal of Veterinary Internal Medicine, 18 , 301-306. Reference 9. Eggertsdóttir A.V., Lund H.S., Krontveit R., Sørum H., (2007). Bacteriuria in cats with feline lower urinary tract disease: a clinical study of 134 cases in Norway. Journal of Feline Medicine and Surgery, 9, 458-65. Reference 10. Emödy L., Kerenyi M., Nagy G., (2003). Virulence factors of uropathogenic Escherichia coli. The International Journal of Antimicrobial Agents, 22, 529-533.Reference 11. Günaydın E., Müştak H.K., Önat K., Diker S., (2017). Evaluation of virulence factors and phylogrouping of Escherichia coli strains isolated from acute bovine mastitis in Turkey. Kafkas Universitesi Veteriner Fakültesi Dergisi, 23, 431-435. Reference 12. Hariharan H., Brathwaite-Sylvester E., Belmar V.M., Sharma R., (2016). Bacterial isolates from urinary tract infection in dogs in Grenada, and their antibiotic susceptibility. Open Journal of Veterinary Medicine, 6, 85-88.Reference 13. Johnson J.R, Brown J.J., (1996). A novel multiply polymerase chain reaction assay for identification of variant papG genes encoding the Gal(α 1-4) Gal-binding papG adhesins of Escherichia coli. The Journal of infectious diseases, 173, 920-926.Reference 14. Johnson J.R., Kaster N., Kuskowski M.A., Gerald V.L., (2003). Identification of urovirulence traits in Escherichia coli by comparison of urinary and rectal E. coli isolates from dogs with urinary tract infection. Journal of Clinical Microbiology, 41, 337-345. Reference 15. Johnson J.R., O’Bryan T.T., Low D.A., Ling G., Delavari P., Fasching C., Russo T.A., Carlino U., Stell A.L., (2000). Evidence of commonality between caine and human extraintestinal pathogenic E. coli strains that express papG Allele III. Infection and Immunity, 68, 3327-3336. Reference 16. Johnson J.R., Stell A.L., (2000). Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and host compromise. The Journal of infectious diseases, 181, 261-72Reference 17. Johnson J.R., Stell A.L., Delavari P., Murray A.C., Kuskowski M., Gaastra W., (2001). Phylogenetic and pathotypic similarities between Escherichia coli isolates from urinary tract infections in dogs and extraintestinal infections in humans. The Journal of infectious diseases, 183, 897-906. Reference 18. Kariyama R., Mitsuhata R., Chow J.W., Clewell D.B., Kumon H., (2000). Simple and reliable Multiplex-PCR assay for surveliance isolates of vancomycin-resistant enterococci. Journal of Clinical Microbiology, 38, 3092-3095. Reference 19. le Bouguenec C., Archambaud M., Labigne A., (1992). Rapid and specific detection of the pap, afa and sfa adhesin-encoding operons in uropathogenic Escherichia coli strains by polymerase chain reaction. Journal of Clinical Microbiology, 30, 1189-1193. Reference 20. Lund H.S., Skogtun G., Sørum H., Eggertsdóttir A.V., (2015). Antimicrobial susceptibility in bacterial isolates from Norwegian cats with lower urinary tract disease. Journal of Feline Medicine and Surgery, 17, 507-51.)Reference 21. NCCLS. (2004) Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria ısolated from animals; Informational Supplement. NCCLS document M31-S1 (ISBN 1-56238-534-8). NCCLS,940 West Valley Road,Suite 1400, Wayne, Pennsylvania 19087-1898,USA.Reference 22. Oluoch A.O., Kim C.H., Weisiger M., Koo H.Y., Siegel A.M., Campbell K.L., Burke T.J., McKiernan B.C., Kakoma I., (2001). Nonenteric Escherichia coli isolates from dogs: 674 cases (1990-1998). Journal of American Veterinary Medical Association, 218, 381-384. Reference 23. Quinn P.J., Carter M.E., Markey B.K., Carter G.R., (1999). Section 18: Enterobactericeae. In: Quinn PJ, Carter ME, Markey BK and Carter GR editors. Clinical Veterinary Microbiology. 3rded. Harcourt Publishers Limited, pp. 209-226.Reference 24. Siqueira A.K., Ribeiro M.G., Leite D.S., Tiba M.R., Moura C., Lopes M.D., Prestes N.C., Salerno T., Silva A.V., (2009). Virulence factors in Escherichia coli strains isolated from urinary tract infection and pyometra cases and from feces of healthy dogs. Research in Veterinary Science, 86, 206-210. Reference 25. Thompson M.F., Litster A.L., Platell J.L., Trott D.J., (2011). Canine bacterial urinary tract infections: new developments in old pathogens. The Veterinary Journal, 190, 22-27. Reference 26. Tramuta C., Nucera D., Robino P., Salvarani S., Nebbia P., (2011). Virulence factors and genetic variability of uropathogenic Escherichia coli isolated from dogs and cats in Italy. The Journal of Veterinary Science, 12, 49-55.Reference 27. Tsai Y.L., Palmer C.J., Sangrmano L.R., (1993). Detection of Escherichia coli in sewage and sludge by polymerase chain reaction. Applied and Environmental Microbiology, 59, 353.Reference 28. Wells J.E., Bartges J.W., Kania S.A., Bemis D.A., Gluhak T., (2013). Association between presence of urovirulence factors, phylogenetic class, and antimicrobial resistance patterns in 159 uropathogenic Escherichia coli samples isolated from dogs. Open Journal of Veterinary Medicine, 3, 199-203.Reference 29. Yamamoto S., Terai A., Yuri K., Kurazono H., Takeda Y., Yoshida O., (1995). Detection of urovirulence factors in Escherichia coli by multiplex polymerase chain reaction. FEMS Immunology and Medical Microbiology, 12, 85-90. Reference 30. Yuri K., Nakata K., Katae H., Yamamoto S., Hasegawa A., (1998). Distribution of uropathogenic virulence factors among Escherichia coli strains isolated from dogs and cats. Journal of Veterinary Medical Sciences, 60, 287-290.