Kurşuna Maruz Bırakılan Gammarus pulex’de Antioksidan Enzim Yanıtları

Yıl 2019, Cilt: 4 Sayı: 2, 216 - 220, 31.08.2019

Osman Serdar Nuran Cıkcıkoğlu Yıldırım , Şule Tatar , Numan Yıldırım

https://doi.org/10.35229/jaes.571909

Cited By: 1

Öz

Bu çalışmada, kurşuna
maruz bırakılan G. pulex'de
süperoksit dismutaz, katalaz ve glutatyon peroksidaz antioksidan enzimlerinin
aktivite yanıtlarının araştırılması amaçlanmaktadır.  G.
pulex, 96 saat boyunca 10, 30, 50 µg/L kurşun içeren sentetik çözeltilere
maruz bırakılmıştır.  Süperoksit
dismutaz, katalaz ve glutatyon peroksidaz aktiviteleri ELISA kiti kullanılarak
belirlenmiştir.  Süperoksit dismutaz
aktivitelerindeki değişimler istatistiksel olarak anlamlı bulunmamıştır (P>0.05). Kurşuna maruz bırakıldıktan
sonra tüm uygulama gruplarında katalaz aktiviteleri azalmıştır (P<0.05).  Glutatyon peroksidaz aktiviteleri kurşun
maruziyetine bağlı olarak azalmıştır (P>0.05).  Bulgularımız, reaktif oksijen türleri
üreterek ve sülfhidril bağımlı antioksidan enzim aktivitelerini inhibe ederek
kurşunun oksidatif strese neden olabileceğini göstermektedir.  Sonuç olarak, antioksidan enzimlerdeki
değişiklikler, kurşunun çevresel risk değerlendirmesi için potansiyel olarak
hassas biyobelirteçler olarak kullanılabilir ve deşarj standartlarının
oluşturulmasına katkıda bulunabilir.

Anahtar Kelimeler

G. pulex, Süperoksit Dismutaz, Katalaz, Glutatyon Peroksidaz

Antioxidant Enzyme Responses to Lead Exposure of Gammarus pulex

Öz

It is
aimed to investigate the activity response of the antioxidant enzymes
superoxide dismutase, catalase and glutathione peroxidase of G. pulex exposed to lead.  The
G. pulex were exposed synthetic
solutions containing 10, 30, 50 µg/L lead for 96 h.  Superoxide dismutase, catalase, glutathione
peroxidase activities were determined by using ELISA kit.  The differences in
superoxide dismutase activities were statistically insignificant (P>0.05).  Catalase decreased in all treatment groups
after lead exposure (P<0.05).  The glutathione peroxidase activities were
decreased depending on lead exposure (P>0.05).  Our
findings suggest that lead can induce oxidative stress by generating reactive
oxygen species and inhibiting sulfhydryl dependent antioxidant enzymes
activities.  In conclusion, alterations
in antioxidant enzymes may potentially be used as sensitive biomarkers for risk
assessment of lead in the environment and may contribute to the establishment
of discharge regulations.

Anahtar Kelimeler

G. pulex, Superoxide Dismutase, Catalase, Glutathione Peroxidase

Kaynakça

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