Türkiye’nin Samsun İlindeki Bazı Yabani Baklagil Türlerini Nodüle Eden Mesorhizobium Türlerinin Çeşitliliği

Yıl 2019, Cilt: 4 Sayı: 3, 401 - 408, 30.12.2019

Okan Kadir Nohut Cem Tolga Gürkanlı İbrahim Özkoç

https://doi.org/10.35229/jaes.599593

Öz

Bu çalışmada Türkiye’nin Samsun
ilinden toplanan iki yabani Baklagil türünün (Argyrolobium biebersteinii (Ball in Feddes) and Lotus angustissimus
L.,)
mikrosimbiyontları geleneksel ve moleküler yöntemler kullanılarak incelenmiştir.
Toplamda orijinal konakçıları üzerinde aktif kök nodülleri oluşturan beş adet
rizobiyal izolat incelenmiştir. Bu izolatların teşhisleri 16S rDNA ve recA olmak üzere iki genin filogenetik
analizleri ile yapılmıştır. Sonuç olarak OKN-1 ve OKN-4 Mesorhizobium
tarimense ve OKN-3 M. japonicum
olarak teşhis edilmişlerdir. Diğer taraftan diğer iki izolatımız OKN-7 ve
OKN-10 potansiyel iki yeni Mesorhizobium türü olarak ortaya çıkmıştır. Sonuç olarak bu çalışmada Türkiye’den Mesorhizobium
cinsine ait ilk moleküler olarak tanımlanmış örnekleri sunmaktayız. Ayrıca M.
tarimense ve M. japonicum türleri için Türkiye’den ve Avrupa’dan ilk
kayıtları da vermekteyiz. Ek olarak M. tarimense ve M. japonicum izolatlarımız,
bu iki türün orijinal konaklarından ve lokalitelerinden tanımlanmasından sonra
bildirilen ilk izolatlardır. Bu çalışmada potansiyel olarak yeni iki Mesorhizobium
türü için de moleküler kanıtlar sunuyoruz.

Anahtar Kelimeler

Filogeni, Mesorhizobium, Nodülasyon, Samsun

Destekleyen Kurum

Ondokuz Mayıs Üniversitesi

Proje Numarası

PYO.FEN.1904.10.006

Diversity of Mesorhizobium Species Nodulating Some Wild Legumes in Samsun Province of Turkey

Öz

In this study microsymbionts of
two wild legumes, Argyrolobium
biebersteinii (Ball
in Feddes) and Lotus
angustissimus L.,
collected from Samsun province of Turkey were investigated using conventional
and molecular methods. A total of five rhizobial isolates which produced active
root nodules on their original hosts were considered. Identifications of the
isolates were depending on the phylogenetic analyses of two housekeeping genes,
16S rDNA and recA. As a result,
isolates OKN-1 and OKN-4 identified as Mesorhizobium
tarimense and OKN-3 identified as M.
japonicum. On the other hand, our other two isolates OKN-7 and OKN-10
appeared as two potentially new Mesorhizobium species. In this study we present the first Mesorhizobium isolates identified using valid molecular methods from Turkey. We also
give the first reports of M. tarimense and M. japonicum from Turkey
and Europe. Additionally, our M. tarimense and M. japonicum
isolates are the first isolates reported after the description of these two
species from their original hosts and locations. In this study we also present
molecular evidences for two potentially new Mesorhizobium species.

Anahtar Kelimeler

Mesorhizobium, Nodulation, Phylogeny, Samsun

Proje Numarası

PYO.FEN.1904.10.006

Kaynakça

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