Yıl 2019, Cilt 4 , Sayı 3, Sayfalar 480 - 483 2019-12-30

Preparation of O-antigen from Yersinia ruckeri Serotype O1 and Use in the Slide Agglutination Test

Fikri BALTA [1] , Zeynep DENGİZ BALTA [2]


In this study, it was aimed at early detection of yersiniosis caused by Yersinia ruckeri in rainbow trouts (Oncorhynchus mykiss) using agglutination test. O-antigen of Y. ruckeri isolated from diseased fishes was prepared. This prepared O-antigen was injected into the ear vein of the rabbit. Serum was removed from blood samples taken on 7th and 15th days after the last injection. Thus, the antibody of the Yersinia ruckeri was produced in the rabbit. Titers of the resulting antibodies were determined by microtitering in 96 well-based plates. It was used successfully in the slide agglutination tests by making necessary dilutions according to the titer ratios of the antibodies. We determined that the motile and Gr (-) bacteria isolated from diseased rainbow trout with yersiniozis symptom were Y. ruckeri according to the results of slide agglutination test without any biochemical test. The isolated bacteria were confirmed to be Y. ruckeri with the results of API 20E test kit and PCR test. The antibodies were stored at -20 and -80°C for use subsequent studies.

Yersinia ruckeri, O antigen, slide agglutination test
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Birincil Dil en
Konular Fen
Bölüm Makaleler
Yazarlar

Orcid: 0000-0002-1823-5823
Yazar: Fikri BALTA (Sorumlu Yazar)
Kurum: RECEP TAYYİP ERDOĞAN ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0002-7795-828X
Yazar: Zeynep DENGİZ BALTA
Kurum: RECEP TAYYİP ERDOĞAN ÜNİVERSİTESİ, FEN BİLİMLERİ ENSTİTÜSÜ
Ülke: Turkey


Tarihler

Yayımlanma Tarihi : 30 Aralık 2019

Bibtex @araştırma makalesi { jaes645416, journal = {Journal of Anatolian Environmental and Animal Sciences}, issn = {}, eissn = {2548-0006}, address = {}, publisher = {Bülent VEREP}, year = {2019}, volume = {4}, pages = {480 - 483}, doi = {10.35229/jaes.645416}, title = {Preparation of O-antigen from Yersinia ruckeri Serotype O1 and Use in the Slide Agglutination Test}, key = {cite}, author = {BALTA, Fikri and DENGİZ BALTA, Zeynep} }
APA BALTA, F , DENGİZ BALTA, Z . (2019). Preparation of O-antigen from Yersinia ruckeri Serotype O1 and Use in the Slide Agglutination Test. Journal of Anatolian Environmental and Animal Sciences , 4 (3) , 480-483 . DOI: 10.35229/jaes.645416
MLA BALTA, F , DENGİZ BALTA, Z . "Preparation of O-antigen from Yersinia ruckeri Serotype O1 and Use in the Slide Agglutination Test". Journal of Anatolian Environmental and Animal Sciences 4 (2019 ): 480-483 <https://dergipark.org.tr/tr/pub/jaes/issue/50506/645416>
Chicago BALTA, F , DENGİZ BALTA, Z . "Preparation of O-antigen from Yersinia ruckeri Serotype O1 and Use in the Slide Agglutination Test". Journal of Anatolian Environmental and Animal Sciences 4 (2019 ): 480-483
RIS TY - JOUR T1 - Preparation of O-antigen from Yersinia ruckeri Serotype O1 and Use in the Slide Agglutination Test AU - Fikri BALTA , Zeynep DENGİZ BALTA Y1 - 2019 PY - 2019 N1 - doi: 10.35229/jaes.645416 DO - 10.35229/jaes.645416 T2 - Journal of Anatolian Environmental and Animal Sciences JF - Journal JO - JOR SP - 480 EP - 483 VL - 4 IS - 3 SN - -2548-0006 M3 - doi: 10.35229/jaes.645416 UR - https://doi.org/10.35229/jaes.645416 Y2 - 2019 ER -
EndNote %0 Journal of Anatolian Environmental and Animal Sciences Preparation of O-antigen from Yersinia ruckeri Serotype O1 and Use in the Slide Agglutination Test %A Fikri BALTA , Zeynep DENGİZ BALTA %T Preparation of O-antigen from Yersinia ruckeri Serotype O1 and Use in the Slide Agglutination Test %D 2019 %J Journal of Anatolian Environmental and Animal Sciences %P -2548-0006 %V 4 %N 3 %R doi: 10.35229/jaes.645416 %U 10.35229/jaes.645416
ISNAD BALTA, Fikri , DENGİZ BALTA, Zeynep . "Preparation of O-antigen from Yersinia ruckeri Serotype O1 and Use in the Slide Agglutination Test". Journal of Anatolian Environmental and Animal Sciences 4 / 3 (Aralık 2020): 480-483 . https://doi.org/10.35229/jaes.645416
AMA BALTA F , DENGİZ BALTA Z . Preparation of O-antigen from Yersinia ruckeri Serotype O1 and Use in the Slide Agglutination Test. JAES. 2019; 4(3): 480-483.
Vancouver BALTA F , DENGİZ BALTA Z . Preparation of O-antigen from Yersinia ruckeri Serotype O1 and Use in the Slide Agglutination Test. Journal of Anatolian Environmental and Animal Sciences. 2019; 4(3): 483-480.