Cu, Zn ve Karışımlarının Galleria mellonella L. (Lepidoptera: Pyralidae) Larvalarının Hemosit Tipleri ve Sayıları Üzerine Etkileri

Yıl 2020, Cilt: 5 Sayı: 1, 45 - 51, 31.03.2020

Benay Tunçsoy Ayşe Kara Pinar Özalp

https://doi.org/10.35229/jaes.649536

Cited By: 1

Öz

Yapılan
çalışmada, Zn (30 mg/L) ve Cu (10 mg/L)’ nun tek başına ve karışım halinde
toksik etkilerinin Galleria mellonella larvalarının
hemosit tipleri ile total ve diferansiyel hemosit sayıları üzerine olan
etkileri incelenmiştir.

            G.mellonella
larvalarında prohemosit, granülosit, plazmatosit, sferülosit ve önositoid olmak
üzere beş tip hemosit gözlenmiştir. Total ve diferansiyel hemosit sayıları ağır metallerden önemli ölçüde etkilenmiştir. Metallerin
uygulanması sonucunda (72 saat sonunda) total hemosit sayısı (THS) azalmıştır.
Prohemosit, granülosit, sferülosit ve önositoidlerde azalma plazmatosit
sayılarında ise artış gözlenmiştir.

            G.mellonella
larvalarının hemosit sayılarında meydana gelen değişimler çevre kirliliği düzeylerini değerlendirmede iyi bir
model organizma olarak kullanılabilirliğini ve başka türler üzerinde de
yapılacak immünolojik çalışmalara yol gösterebileceğini ortaya koymuştur.

Anahtar Kelimeler

Bakır, Çinko, Galleria mellonella

Destekleyen Kurum

Çukurova Üniversitesi BAP birimi

Proje Numarası

FYL-2018-10328

Effects of Cu, Zn and its mixtures on Hemocyte Types and Counts of Galleria mellonella L. Larvae (Lepidoptera: Pyralidae)

Öz

Toxic
effects of Zn (30 mg / L) and Cu(10mg / L) applied singly and in mixture were
investigated on the total and differential hemocyte counts of Galleria mellonella larvae.

            Five types of hemocytes were
observed in G. mellonella larvae;
prohemocytes, granulocytes, plasmatocytes, spherulocytes and eunocytoids. Total
and differential hemocyte counts were significantly affected by heavy metals.
Total hemocyte count (THC) decreased when larvae exposed to metal for (72
hours).  Decreased  prohemocytes, granulocytes, spherulocytes and
eunocytoids whereas plasmatocyte numbers increased.

            Changes in hemocyte numbers of G. mellonella larvae have shown that it
can be used as a good model organism in evaluating environmental pollution
levels and it can also lead to immunological studies on other species.

Anahtar Kelimeler

Copper, Zinc, Galleria mellonella

Proje Numarası

FYL-2018-10328

Kaynakça

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