Türkiye’deki Fırat Nehri Drenajından Oxynoemachelius (Teleostei: Nemacheilidae) Türlerinin COI-barkod Bölgesine Dayalı Genetik Tanımlaması ve Filogenetik İlişkileri

Yıl 2020, Cilt: 5 Sayı: 3, 408 - 418, 30.09.2020

Hande Rakıcı İsmail Aksu Yusuf Bektaş

https://doi.org/10.35229/jaes.776381

Cited By: 2

Öz

Bu çalışmada, DNA barkodlama tekniği ile Türkiye’nin Fırat nehir havzasındaki Oxynoemacheilus türlerini güvenilir bir şekilde tanımlama yeteneği araştırılmıştır. Oxynoemacheilus genusunu temsilen 6 türe ait 167 bireyi barkodlamak için mitokondri sitokrom c oksidaz altünite I' in (COI) barkod bölgesi (627 bp) kullanıldı. Kimura 2 parametresine dayalı olarak (K2P), maksimum tür içi ve minimum türlerarası genetik mesafeleri sırasıyla % 0.59 ve % 1.62‘dır. En yakın komşu türler arası mesafeler, ortalama tür içi mesafeden 7 kat daha yüksektir ve belirgin bir DNA barkod aralığı fark edilmiştir. Filogeni tabanlı DNA barkodlama başarısı testine göre komşu-birleştirme fenogramları K2P modeli kullanılarak 1000 seç-bağla replikasyonu ile üretildi ve örneklerin tümünün türlerin taksonomik durumları ile uyumlu olarak kümelendiği belirlenmiştir. MP ve ML filogenileri, Doğu ve Batı Anadolu gruplarına karşılık gelen iki klad içeren bir ortak ağaç topolojisi gösterdi. Bu çalışmanın sonuçları, COI geninin yaklaşık % 81 başarı oranıyla Oxynoemacheilus türlerinin belirlenmesi ve sınırlandırılması için uygun bir DNA barkod belirleyicisi olabileceğini göstermektedir.

Anahtar Kelimeler

COI, COI, Endemizm, taş ısıran balığı, taş ısıran balığı, taş ısıran balığı, Türkiye

Destekleyen Kurum

Recep Tayyip Erdoğan Üniversitesi

Proje Numarası

2015.53008.103.01.01

Teşekkür

Bu proje Recep Tayyip Erdoğan Üniversitesi Bilimsel Araştırmalar Birimi tarafından 2015.53008.103.01.01 nolu ile proje desteklenmiştir. Prof. Dr. Davut Turan, Dr. Cüneyt Kaya ve Dr. Esra Bayçelebi'ye arazi ve morfolojik teşhis konusundaki yardımlarından dolayı teşekkür ederiz.

Genetic identification and phylogenetic relations of Oxynoemachelius species (Teleostei: Nemacheilidae) from drainage of Euphrates in Turkey based on COI-barcode region

Öz

The present study investigated the ability of DNA barcoding to reliably identify Oxynoemacheilus species in Euphrates River basin of Turkey, known as biodiversity hotspots. The barcode region (627 bp) of the mitochondrial cytochrome c oxidase I (COI) were used to barcode 167 individuals belonging to six Oxynoemacheilus species. Based on Kimura two-parameter (K2P), the intraspecific diversity and minimuminterspecific genetic distance were 0.59% and 1.62% respectively. Nearest neighbor distance was 7-fold higher than maximum intra-specific distance on average and a distinct DNA barcoding gap was observed.According to the phylogeny-based succes test neighbour-joining phenograms were generated with 1000 bootstrap replications using the K2P model and all the specimens clustered in concurrence with the taxonomic status of the species. The MP and ML phylogenies indicated a consensus tree topology containing two cladescorresponding to the geographical origins: Western and Eastern Anatolia groups. The results of present study indicated that the COI gene could be a suitable DNA barcode marker for the Oxynoemacheilus species identification and delimitation with approximately 81% success rate.

Anahtar Kelimeler

DNA BArcoding, Turkey, Spined loach, DNA barkodlama, DNA barkodlama, COI, COI, COI, COI, taş ısıran balığı, taş ısıran balığı, taş ısıran balığı, taş ısıran balığı

Proje Numarası

2015.53008.103.01.01

Kaynakça

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