Genetic analysis of Carasobarbus Karaman, 1971 (Actinopterygii Cyprinidae) in Anatolia

Yıl 2020, Cilt: 5 Sayı: 4, 520 - 526, 31.12.2020

Didem Bahadır İsmail Aksu , Yusuf Bektaş

https://doi.org/10.35229/jaes.796051

Cited By: 1

Öz

We sequenced the complete mitochondrial cytochrome b gene (1141 bp) of 70 individuals from ten populations of three Carasobarbus species in Anatolia. Totally eight haplotypes were identified. The intraspecies genetic distance ranged from 0.00% to 0.21%, while it varied from 2.6% to 9.0% for interspecies. Except for Gaziantep samples with low sample size, high haplotype diversity (Hd= 0,590-0,833) and low nucleotide diversity (Pi= 0,05-0,65) values can be explained by the presence of small populations sensitive to genetic drift and founder effects. Phylogenetic analyses constructed with neihgbour joining, maximum likelihood and maximum parsimony generated similar topologies supported by high bootstrap values. Phylogenetic tree topologies showed that the C. apoensis haplotype was located in the C. luteus species. Therefore, the validity of species status of C. apoensis should be checked morphologically. On the other hand, since the Kahta population in C. luteus has a remarkably high genetic diversity, it must be re-evaluated morphologically. The tectonic uplift of the Anatolian Plateau between the African and European plates during the Pliocene period may have probably prevented the presence of Carasobarbus in the west of the Anatolian diagonal.

Anahtar Kelimeler

Anatolia, Carasobarbus, Phylogeny, mtDNA

Destekleyen Kurum

Recep Tayyip Erdoğan Üniversitesi

Proje Numarası

2015.53008.103.01.01

Teşekkür

This work was funded by the scientific research funds of Recep Tayyip Erdogan University (RTEÜ BAP, Project No: 2015.53008.103.01.01).

Anadolu’daki Carasobarbus Karaman, 1971 (Actinopterygii Cyprinidae) genusunun genetik analizi

Öz

Anadolu'daki üç Carasobarbus türünün 10 populasyonundan 70 bireyin mitokondri sitokrom b geninin (1141 bp) DNA dizin analizi yapılmıştır. Toplamda sekiz haplotip tanımlandı. Sitokrom b veri analizi kullanılarak, tür içi genetik mesafenin % 0,00 ile % 0,21 arasında, türler arası için ise % 2,6 ile% 9,0 arasında değiştiği belirlenmiştir. Yeterli örneklem büyüklüğüne sahip olmayan Gaziantep örnekleri hariç yüksek haplotip çeşitlilik (Hd= 0,590-0,833) ve düşük nükleotit çeşitlilik (Pi= 0,05-0,65) değerleri, genetik sürüklenme ve kurucu etkilere karşı duyarlı olan küçük populasyonların varlığı ile açıklanabilir. Komşu birleştirme, maksimum tutumluluk ve maksimum olasılık metotları yoluyla oluşturulan filogenetik analizler yüksek güvenirlilik değerleri ile desteklenen benzer topolojiler üretmiştir. Filogenetik ağaç topolojileri gösterdi ki C. apoensis haplotipleri C. luteus haplotipleri içinde konumlandığı için türün geçerliliği morfolojik olarak kontrol edilmelidir. Diğer taraftan, C. luteus içindeki Kahta populasyonu dikkate değer düzeyde yüksek genetik çeşitliğe sahip olduğundan morfolojik olarak mutlaka yeniden değerlendirilmelidir. Pliyosen döneminde Afrika ve Avrupa levhaları arasındaki çarpışmadan kaynaklanan Anadolu Platosu’nun tektonik yükselişi muhtemelen Anadolu köşegeninin batısında Carasobarbus’un varlığını engellemiştir.

Anahtar Kelimeler

Anadolu, Carasobarbus, Filogeni, Türkiye

Proje Numarası

2015.53008.103.01.01

Kaynakça

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