Hastalık Semptomları Gösteren Gökkuşağı Alabalıklarından İzole Edilen Chryseobacterium piscicola İzolatlarının Fenotipik, Filogenetik Karakterizasyonu ve Antimikrobiyal Duyarlılıklarının Belirlenmesi

Yıl 2020, Cilt: 5 Sayı: 4, 624 - 629, 31.12.2020

Izzet Burcin Saticioglu Soner Altun Muhammed Duman

https://doi.org/10.35229/jaes.808537

Cited By: 3

Öz

Çalışmamızda iştahsızlık, ekzoftalmus, renkte kararma ve sırt yüzgeci erozyonu belirtileri gösteren 1-4 gram ağırlıkları arasında gökkuşağı alabalıklarından izole edilen oniki izolat kullanılmıştır. Konvansiyonel mikrobiyolojik testlerin yanı sıra Biolog GENIII mikroplate kullanılarak geniş kapsamlı fenotipik karakterizasyon yapılmıştır. 16S rRNA bölgesi kullanılarak moleküler identifikasyon ve karakterizasyon yapılmıştır. Çalışmada kullanılan izolatların antimikrobiyal duyarlılıkları disk difüzyon yöntemi kullanılarak belirlenmiştir. 16S rRNA bölgesine dayalı dizi analizi ile yapılan moleküler identifasyonda izolatlarımız %99 oranında Chryseobacterium piscicola olarak tanımlanmıştır. İzolatlarımız, Amerika, Şili ve Finlandiya’daki üç farklı konakçıdan elde edilen izolatlarla yapılan filogenetik analizde yüksek benzerlik oranı ile beş farklı genogrup belirlenmiştir. Finlandiya, Şili ve ABD'den izole edilen izolatlar ve C-316 (Türkiye) izolatı aynı genogrupta bulunmuştur. 16S rRNA bölgesi ile oluşturulan filogenetik analizin bakterinin izole edildiği konakçı ayrımının yapamadığı görülmüştür. Filogenetik analize göre seçilen altı temsili izolatın fenotipik özellikleri Biolog GENIII mikroplakası ile belirlenmiştir. Temsili izolatların Biolog GENIII sonuçlarına göre, 94 testin 40'ının sonuçlarının değişken olduğu bulunmuştur. Bu sonuçla, C. piscicola izolatlarının fenotipik olarak homojen bir yapıda olmadığı görülmüştür. Ayrıca çalışmamızda izolatlarımızın florfenikol, enrofloksasin ve sülfametoksazol/trimetoprime karşı oluşan zon çaplarının diğerlerine göre daha yüksek olduğu ve C-41’in en dirençli izolat olduğu bulunmuştur.

Anahtar Kelimeler

Flavobacteriaceae, Chryseobacterium piscicola, Gökkuşağı alabalığı

Destekleyen Kurum

Erciyes Üniversitesi Bilimsel Araştırmalar Projeleri Birimi

Proje Numarası

TCD-2018-8586

Phenotypic, Phylogenetic Characterization and Antimicrobial Susceptibility Determination of Chryseobacterium piscicola Isolates Recovered from Diseased Rainbow Trout

Öz

Twelve isolates recovered from rainbow trout weighing 1-4 grams showing signs of anorexia, exophthalmos, darkening, and dorsal fin erosion were used in our study. In addition to conventional microbiological tests, comprehensive phenotypic characterization has been performed using the Biolog GEN III microplate. Molecular identification and characterization were performed using the 16S rRNA region. Antimicrobial susceptibilities of the isolates were determined using the disk diffusion method. Our isolates were identified as Chryseobacterium piscicola in molecular identification performed by sequence analysis based on the 16S rRNA region. In a phylogenetic analysis of our isolates, obtained from three different hosts in America, Chile, and Finland, five genogroups were determined with high similarity rate. Isolates from Finland, Chile, the United States, and Turkey (only C-316) were found in the same genogroup. It was determined that the phylogenetic analysis created with the 16S rRNA region could not distinguish the host from which the bacteria was isolated. The phenotypic characterization of six representative isolates selected according to phylogenetic analysis, was determined with the Biolog GENIII microplate. Based on the Biolog GENIII results of the representative isolates, the results of 40 out of 94 tests were found to be variable. With this result, it was found that C. piscicola isolates were not phenotypically homogeneous. Besides, it was found that the zone diameters of our isolates against florfenicol, enrofloxacin, and sulfamethoxazole/trimethoprim were higher than the other isolates, in addition to that C-41 was the most resistant isolate.

Anahtar Kelimeler

Flavobacteriaceae, Chryseobacterium piscicola, Rainbow Trout

Proje Numarası

TCD-2018-8586

Kaynakça

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