Cyfluthrin Pestisitinin Zebra Midyesi (Dreissena polymorpha) Üzerindeki Etkisinin Bazı Antioksidan Enzim Aktiviteleriyle Belirlenmesi

Yıl 2021, Cilt: 6 Sayı: 1, 77 - 83, 31.03.2021

Osman Serdar

https://doi.org/10.35229/jaes.804479

Cited By: 5

Öz

Yapılan bu çalışmada, Dreissena polymorpha’da ticari insektisit olan Cyfluthrin (CFT)’nin bazı biyokimyasal yanıtları araştırılmıştır. CFT’nin D. polymorpha üzerindeki 96 saatlik LC50 değeri 553,22 ± 27,3 µg/L olarak hesaplanmıştır. D. polymorpha, CFT’nin subletal konsantrasyonlar (LC50 değerinin 1/20, 1/10 ve 1/5)’ına 24 ve 96 saat süre ile maruz bırakılmıştır. D. polymorpha bireylerinde superoksit dismustaz (SOD), katalaz (CAT) ve Glutatyon peroksidaz (GPx) enzim aktiviteleri belirlenmiştir. CFT’ye maruz bırakılan D. polymorpha’da, SOD aktivitesi kontrole kıyasla artmıştır. CAT ve GPx aktivitelerinin kontrole kıyasla inhibe olduğu belirlenmiştir.
Sonuç olarak, CFT'ye maruz kalan D. polymorpha bireylerinde oksidatif hasarda artışa olduğu bulunmuştur.

Anahtar Kelimeler

Dreissena polymorpha, Cyfluthrin, Glutatyon peroksidaz, Superoksit dismustaz

Destekleyen Kurum

Munzur Üniversitesi Bilimsel Araştırmalar Proje Birimi

Proje Numarası

PPMUB019-17.

Determination of the Effect of Cyfluthrin Pesticide on Zebra Mussel (Dreissena polymorpha) by Some Antioxidant Enzyme Activities

Öz

In this study, some biochemical responses of Cyfluthrin (CFT), a commercial insecticide in Dreissena polymorpha, were investigated. The 96 hour LC50 value of CFT on D. polymorpha was calculated as 553.22 ± 27.3 µg / L. D. polymorpha was exposed to subletal concentrations (1/20, 1/10 and 1/5 of LC50 value) of CFT for 24 and 96 hours. The enzyme activities of superoxide dismustase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were determined in D. polymorpha individuals. In D. polymorpha exposed to CFT, SOD activity increased compared to control. It was determined that CAT and GPx activities were inhibited compared to the control.
As a result, D. polymorpha individuals exposed to CFT were found to cause increased oxidative damage.

Anahtar Kelimeler

Dreissena polymorpha, Catalase, Cyfluthrin, Glutathione peroxidase, Superoxide dismustase

Proje Numarası

PPMUB019-17.

Kaynakça

• Ahmad, I., Hamid, T., Fatima, M., Chand, H.S., Jain, S.K., Athar, M. & Raisuddin, S. (2000). Induction of hepatic antioxidants in freshwater catfish (Channa punctatus Bloch) is a biomarker of paper mill effluent exposure. Biochimica et Biophysica Acta (BBA)-General Subjects, 1523(1), 37-48.
• Almeida, J.A., Diniz, Y.S., Marques, S.F.G., Faine, L.A., Ribas, B.O., Burneiko, R.C. & Novelli, E.L.B. (2002). The use of the oxidative stress responses as biomarkers in Nile tilapia (Oreochromis niloticus) exposed to in vivo cadmium contamination. Environment International, 27(8):673–679
• Bainy, A.C.D., Arisi, A.C.M., Azzalis, L.A., Simizu, K., Barros, S.B.M., Videla, L.A. & Junqueira, V.B.C. (1993). Differential effects of short-term lindane administration on parameters related to oxidative stress in rat liver and erythrocytes. Journal of biochemical toxicology, 8(4):187–194
• Ballesteros, M.L., Wunderlin, D.A. & Bistoni, M.A. (2009). Oxidative stress responses in different organs of Jenynsia multidentata exposed to endosulfan. Ecotoxicology and Environmental Safety, 72(1):199–205
• Bilgin, M., & Uluturhan, E.S. (2015). Homa Dalyan'ında (İzmir Körfezi) dağılım gösteren Mytilus galloprovincialis ve Tapes decussatus (Bivalvia) türlerinde ağır metal birikimlerinin incelenmesi, Su Ürünleri Dergisi, 32(1), 1-8.
• Binelli, A., Della Torre, C., Magni, S., & Parolini, M. (2015). Does zebra mussel (Dreissena polymorpha) represent the freshwater counterpart of Mytilus in ecotoxicological studies? A critical review, Environmental pollution, 196, 386-403.
• Bogan, A.E. (1993). Freshwater bivalve extinctions (Mollusca: Unionoida): a search for causes, American Zoologist, 33(6), 599-609
• Chelikani, P,, Fita, I. & Loewen, P.C. (2004). Diversity of structures and properties among catalases. Cellular and Molecular Life Sciences CMLS, 61(2):192–208
• Cheung, C.C.C., Zheng, G.J., Li, A.M.Y., Richardson, B.J. & Lam, P.K.S. (2001). Relationships between tissue concentrations of polycyclic aromatic hydrocarbons and antioxidative responses of marine mussels, Perna viridis. Aquatic toxicology, 52(3–4):189–203
• Crestani, M., Menezes, C., Glusczak, L., dos Santos Miron, D., Spanevello, R., Silveira, A. & Loro, V. L. (2007). Effect of clomazone herbicide on biochemical and histological aspects of silver catfish (Rhamdiaquelen) and recovery pattern. Chemosphere 67(11):2305–2311
• Dimitrova, M.S., Tishinova, V. & Velcheva, V. (1994). Combined effect of zinc and lead on the hepatic superoxide dismutase-catalase system in carp (Cyprinus carpio). Comp Biochem Physiol C: Pharmacol Toxicol Endocrinol 108(1):43–46
• Doyotte, A., Cossu, C., Jacquin, M.C., Babut, M. & Vasseur, P. (1997). Antioxidant enzymes, glutathione and lipid peroxidation as relevant biomarkers of experimental or field exposure in the gills and the digestive gland of the freshwater bivalve Unio tumidus. Aquatic Toxicology 39(2):93–110
• Durmaz H, Sevgiler Y, Üner N (2006). Tissue-specific antioxidative and neurotoxic responses to diazinon in Oreochromis niloticus. Pestic Biochem Physiol 84(3):215–226
• Erguven, O.G., Tatar, Ş., Serdar, O. & Yildirim, N. C. (2020). Evaluation of the efficiency of chlorpyrifos-ethyl remediation by Methylobacterium radiotolerans and Microbacterium arthrosphaerae using response of some biochemical biomarkers. Environmental Science and Pollutation Research. https://doi.org/10.1007/s11356-020-10672-9
• Fatima, M., Ahmad, I., Sayeed, I., Athar, M. & Raisuddin, S. (2000). Pollutant-induced over-activation of phagocytes is concomitantly associated with peroxidative damage in fish tissues. Aquatic Toxicology 49(4):243–250
• Glusczak, L., dos Santos Miron, D., Moraes, B.S., Simões, R.R., Schetinger, M.R.C., Morsch, V.M. & Loro, V.L. (2007). Acute effects of glyphosate herbicide on metabolic and enzymatic parameters of silver catfish (Rhamdia quelen). Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 146(4):519–524
• Halliwell, B., & Gutteridge, J.M. (2015). Free radicals in biology and medicine”, Oxford University Press, USA.
• Hasspieler, B.M., Behar, J.V., Carlson, D.B., Watson, D.E. & Di Giulio, R.T. (1994). Susceptibility of channel catfish (Ictalurus punctatus) and brown bullhead (Ameriurus nebulosus) to oxidative stress: a comparative study. Aquatic Toxicology 28(1–2):53–64
• Kappus, H. (1985). Lipid peroxidation: mechanisms, analysis, enzymology and biological relevance. Oxidative Stress 273
• Kohen, R. & Nyska, A. (2002). Invited review: Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicologic pathology, 30(6), 620-650.
• Kinzelbach, R.A.G.N.A.R. (1992). The main features of the phylogeny and dispersal of the zebra mussel Dreissena polymorpha”, pp. 5-17. In: D. Neumann and H. A. Jenner (eds.). The Zebra Mussel Dreissena polymorpha: Ecology. Biological Monitoring and First Applications in the Water Quality Management. Gustav Fisher, Stuttgart.
• Livingstone, D.R. (2003). Oxidative stress in aquatic organisms in relation to pollution and aquaculture, Revue de Medecine Veterinaire, 154(6), 427-430.
• Lushchak, V.I. (2011). Environmentally induced oxidative stress in aquatic animals, Aquatic toxicology, 101(1), 13-30.
• Monteiro, D.A., De Almeida, J.A., Rantin, F.T. & Kalinin, A.L. (2006). Oxidative stress biomarkers in the freshwater characid fish, Brycon cephalus, exposed to organophosphorus insecticide Folisuper 600 (methyl parathion). Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 143(2):141–149
• Moreno, I., Pichardo, S., Jos, A., Gomez-Amores, L., Mate, A., Vazquez, C.M. & Camean, A.M. (2005). Antioxidant enzyme activity and lipid peroxidation in liver and kidney of rats exposed to microcystin-LR administered intraperitoneally. Toxicon 45(4):395–402
• Müller S., Wilck N., Kleinewietfeld M., Haase D.N., & Linker, R.A. (2019). Sodium chloride triggers Th17 mediated autoimmunity. Journal of neuroimmunology, 329, 9-13.
• Nishida, Y. (2011). The chemical process of oxidative stress by copper (II) and iron (III) ions in several neurodegenerative disorders, Monatshefte für Chemie-Chemical Monthly, 142(4), 375-384.
• Oliveira, C., Almeida, J., Guilhermino, L., Soares, A.M. & Gravato, C. (2012). Acute effects of deltamethrin on swimming velocity and biomarkers of the common prawn Palaemon serratus. Aquatic Toxicology 124:209–216
• Oruç, E.O., Sevgiler, Y. Üner, & N., (2004). Tissue-specific oxidative stress responses in fish exposed to 2,4-D and azinphosmethyl, Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 137(1): 43–51.
• Pohanka, M. (2011). Cholinesterases, a target of pharmacology and toxicology”, Biomedical Papers of the Medical Faculty of Palacky University in Olomouc, 155(3).
• Rand, G.M. (Ed.). (1995). Fundamentals of aquatic toxicology: effects, environmental fate and risk assessment, CRC press.
• Remacle, J., Lambert, D., Raes, M., Pigeolet, E., Michiels, C. & Toussaint, O. (1992). Importance of various antioxidant enzymes for cell stability. Confrontation between theoretical and experimental data. Biochemical journal, 286(1):41-46
• Sayeed, I., Parvez, S., Pandey, S., Bin-Hafeez, B., Haque, R. & Raisuddin, S. (2003). Oxidative stress biomarkers of exposure to deltamethrin in freshwater fish, Channa punctatus Bloch. Ecotoxicology and environmental safety 56(2):295–301
• Saygı, Ş., (2003). Deneysel toksikolojide toksisite testleri ve test sonuçlarının önemi, Gülhane Tıp Dergisi (GTD), Gülhane Medıcal Journal (GMJ), 291.
• Serdar, O., Yildirim, N. C., Tatar, S., Yildirim, N., & Ogedey, A. (2018). Antioxidant biomarkers in Gammarus pulex to evaluate the efficiency of electrocoagulation process in landfill leachate treatment. Environmental science and pollution research, 25(13), 12538-12544.
• Serdar, O., (2019). The effect of dimethoate pesticide on some biochemical biomarkers in Gammarus pulex. Environmental Science and Pollutation Research 26(21):21905–21914
• Sobjak, T.M., Romão, S., do Nascimento, C.Z., dos Santos, A.F.P., Vogel, L. & Guimarães, A.T.B. (2017). Assessment of the oxidative and neurotoxic effects of glyphosate pesticide on the larvae of Rhamdia quelen fish. Chemosphere 182:267–275
• Slaninova, A., Smutna, M., Modra, H., & Svobodova, Z. (2009). Reviews Oxidative stress in fish induced by pesticides, Neuroendocrinology Letters, 30(1), 2.
• Tatar, S., Cikcikoglu Yildirim, N., Serdar, O., Yildirim, N. & Ogedey, A., (2018). The using of Gammarus pulex as a biomonitor in ecological risk assessment of secondary effluent from municipal wastewater treatment plant in Tunceli, Turkey. Human and Ecological Risk Assessment: An International Journal, 24(3), 819-829.
• Thomas, P.C. & Murthy, T.L. (1976). Studies on the impact of a few organic pesticides on certain fish enzymes. Indian Journal of Animal Sciences, 46(11):619–624
• Tokatlı, C., Emiroğlu, Ö., Arslan, N., Köse, E., Çiçek, A., Dayıoğlu, H., & Başkurt, S. (2016). “Maden Havzası Balıklarında Vücut Ağırlığı ile Ağır Metal Biyoakümülasyon İlişkileri: Emet Çayı Havzası”, Anadolu Üniversitesi Bilim ve Teknoloji Dergisi-C Yaşam Bilimleri ve Biyoteknoloji, 4(2), 57-72.
• Uluturhan, E., Darılmaz, E., Kontas, A., Bilgin, M., Alyuruk, H., Altay, O., & Sevgi, S. (2019). Seasonal variations of multi-biomarker responses to metals and pesticides pollution in M. galloprovincialis and T. decussatus from Homa Lagoon, Eastern Aegean Sea, Marine Pollution Bulletin, 141, 176-186.
• Valavanidis, A., Vlahogianni, T., Dassenakis, M., & Scoullos, M. (2006). Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants, Ecotoxicology and environmental safety, 64(2), 178-189.
• Viarengo, A., Lowe, D., Bolognesi, C., Fabbri, E., & Koehler, A., (2007). The use of biomarkers in monitoring: A 2-tier approach assessing the level of pollutant-induced stress syndrome in sentinel organisms, Comperative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 146:281-300. doi: 10.1016/j.cbpc.2007.04.011
• Wesch, C., Bredimus, K., Paulus, M., & Klein, R. (2016). Towards the suitable monitoring of ingestion of microplastics by marine biota: A review. Environmental Pollution, 218, 1200-1208.
• Wildi, W., Dominik, J., Loizeau, J.L., Thomas, R.L., Favarger, P. ., Haller, L., Perroud, A., & Peytremann, C., (2004). River, reservoir and lake sediment contamination by heavy metals downstream from urban areas of Switzerland. Lakes & Reservoirs: Research & Management, 9(1): 75-87.
• Yildirim, N.C., Tanyol, M., Yildirim, N., Serdar, O. & Tatar, S. (2018). Biochemical responses of Gammarus pulex to malachite green solutions decolorized by Coriolus versicolor as a biosorbent under batch adsorption conditions optimized with response surface methodology. Ecotoxicology and environmental safety 156:41–47
• Yildirim, N. C., Tanyol, M., Serdar, O., & Yildirim, N. (2019). Gammarus pulex as a Model Organism to Assess the Residual Toxicity of Slaughterhouse Wastewater Treated by Electrocoagulation Process. Bulletin of environmental contamination and toxicology, 103(3), 447-452.
• Zhang, J., Shen, H., Wang, X., Wu, J. & Xue, Y. (2004). Effects of chronic exposure of 2, 4-dichlorophenol on the antioxidant system in liver of freshwater fish Carassius auratus. Chemosphere 55(2):167–174.