Antibacterial Effects of Boric Acid Against Aquatic Pathogens

Yıl 2021, Cilt: 6 Sayı: 2, 240 - 244, 30.06.2021

Ecren Uzun Yaylacı

https://doi.org/10.35229/jaes.881144

Öz

Boron is a bioactive trace element generally found in rock, soil, water and air. It is an essential micronutrient for plants besides having beneficial effects on biological functions of human and animal health. Boric acid has antibacterial properties. Thus, the aim of this study was to evaluate the possible bactericidal and bacteriostatic effects of boric acid on the aquatic pathogens: Aeromonas veronii, Photobacterium damselae subsp. damselae, Vibrio anguillarum, Vibrio vulnificus, Vibrio harveyi, Vibrio rotiferianus, Vibrio tubiashii, Vibrio parahaemolyticus, Vibrio furnissii, and Vibrio fluvialis. The inhibitory properties of boric acid were detected by agar well diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods. The results showed that Photobacterium damselae subsp. damselae and Vibrio species were resistant to different concentrations of boric acid. However, boric acid showed an inhibitory effect against Aeromonas veronii at concentrations of 3.09 and 1.54 mg/ml and zone diameters on the agar were measured as 19.4 ± 0.5, 15.92 ± 0.6 mm, respectively. The MIC and MBC of boric acid for Aeromonas veronii were found to be effective at a concentration of 1.54 mg/ml. These results suggested that boric acid might serve as a potential antimicrobial agent for Aeromonas veronii in aquaculture.

Anahtar Kelimeler

antibacterial, aquatic pathogen, boric acid, MIC and MBC

Borik Asitin Sucul Patojenler Üzerine Olan Antibakteriyel Etkileri

Öz

Bor, genellikle kaya, toprak, su ve havada bulunan biyoaktif bir eser elementtir. Borik asidin insan ve hayvan sağlığının biyolojik fonksiyonları üzerinde faydalı etkileri bulunmaktadır. Bitkiler için de vazgeçilmez bir mikro besin olan borik asit antibakteriyel özelliktedir. Bu çalışmanın amacı borik asidin sucul patojenlerden Aeromonas veronii, Photobacterium damselae subsp. damselae Vibrio anguillarum, Vibrio vulnificus, Vibrio harveyi, Vibrio rotiferianus, Vibrio tubiashii, Vibrio parahaemolyticus, Vibrio furnissii ve Vibrio fluvialis’e karşı bakterisidal ve bakteriostatik etkilerini incelemektir. Bu çalışmada, borik asidin inhibisyon özellikleri, agar kuyusu difüzyonu, minimum inhibisyon konsantrasyonu (MİK) ve minimum bakterisidal konsantrasyonu (MBK) yöntemleri kullanılarak tespit edilmiştir. Sonuçlar, Photobacterium damselae subsp. damselae ve Vibrio türlerinin farklı konsantrasyonlardaki borik aside karşı dirençli olduğunu göstermiştir. Bununla birlikte, borik asit 3,09 ve 1,54 mg/ml konsantrasyonlarında Aeromonas veronii’ ye karşı inhibitör etki göstermiştir ve agarda sırasıyla 19,4 ± 0,5 mm ve 15,92 ± 0,6 mm halka çapları ölçülmüştür. Aeromonas veronii için MİK ve MBK değerleri 1,54 mg/ml olarak belirlenmiştir. Bu sonuçlar, borik asidin, su ürünleri yetiştiriciliğinde Aeromonas veronii için potansiyel bir antimikrobiyal ajan görevi görebileceğini ileri sürmektedir.

Anahtar Kelimeler

antibakteriyel, borik asit, MİK ve MBK, sucul patojen

Kaynakça

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