Gökkuşağı Alabalıklarında (Oncorhynchus mykiss) Görülen Lactococcus garvieae Enfeksiyonu ve Tedavisi

Yıl 2021, Cilt: 6 Sayı: 4, 651 - 661, 31.12.2021

İsmail Karatay Fikri Balta

https://doi.org/10.35229/jaes.1024825

Cited By: 2

Öz

Bu çalışma, 2012 Ocak ve 2017 Eylül tarihleri arasında Doğu Karadeniz Bölgesi’ndeki 12 farklı gökkuşağı alabalık çiftliklerinden balıklar toplanmıştır. Hastalıklı gökkuşağı alabalıklarından (Oncorhynchus mykiss) 100 adet bakteri izole edilmiştir. Tüm izolatların tanımlanması geleneksel biyokimyasal testler, api 20 strep testi ve PZR testi kullanılarak tanımlanması yapılmıştır. Pozitif kontrol olarak ATCC 43921 referans izolatı kullanılmıştır. Api 20 strep test sonuçlarına göre izole edilen 100 bakteriden 68 tanesi Lactococcus garvieae olduğu belirlenmiştir. L. garvieae izolatına özel pLG-1 ve pLG-2 (16S rRNA) referans genleri kullanılarak PCR testi ile doğrulanmıştır. Çalışmanın sonucunda, gökkuşağı alabalıklardan izole edilen 100 izolattan 68 adet L. garvieae olduğu teyit edilmiştir. Lactococcus garvieae izolatlarına yapılan antibiyogram test sonuçlarına göre sulfamethoksazol, streptomisin ve sulfametoksazol+trimetoprim’e %100, ampisilin’e % 94.2, oksitetrasiklin %72.5, eritromisin’e % 55.07 ve oksolinik asit % 43.5 dirençli olduğu, fakat enrofloksasin % 26.09, doksisikline %20,3, amoksiklin ve florfenikol % 8.7 daha az düzeyde direnç tespit edilmiştir. Hastalığın tedavisinde üreticiye antibiyogram test sonuçlarına göre en etkili olan antibiyotiklerden florfenikol amoksiklin ve doksisiklinden birinin kullanılması önerilmiştir

Anahtar Kelimeler

Antimikrobiyal ajanlar, api 20 strep, L. garvieae, PZR

Destekleyen Kurum

Recep Tayyip Erdoğan Üniversitesi BAP Birimi

Proje Numarası

2014.103.02.3

Teşekkür

Recep Tayip Erdoğan Üniversitesi, Bilimsel Araştırma Projeleri Birimine teşekkür ederiz.

Lactococcus garvieae Infection and Treatment Observed in Rainbow Trout (Oncorhynchus mykiss)

Öz

In this study, fish were sampled from 12 different fish farms in Eastern Black Sea Region between January 2012 and September 2017. It was isolated hundred bacteria from diseased rainbow trout (Oncorhynchus mykiss). All isolates identification were carryed out by using conventional biochemical tests, api 20 strep tests and PCR tests. ATCC 43921 reference strain was used as a positive control. According to results of the api 20 strep test, it was determined that 68 isolates from 100 bacterial isolates were Lactococcus garvieae. It was confirmed by PCR testing using the reference genes pLG-1 and pLG-2 (16S rRNA) specific to the L. garvieae isolate. As a result of the study, it was confirmed that there were 68 L. garvieae from 100 strains isolated from rainbow trout. The antibiotic susceptibility test results showed that L. garvieae isolates were resistant %100 to streptomycin, sulfamethoxazole and sulfamethoxazole+trimethoprim, 94.2% to ampicillin, 72.5% to oxytetracycline, 55.07% to erythromycin and 43.5% to oxolinic acid, 26.09% to enrofloxacin, % 20.3 doxycycline, 8,7% to amoxicillin and florphenicol. The most effective antibiotic was found to be florfenicol, amoxicillin and doxisicline.

Anahtar Kelimeler

Antimicrobial agents, api 20 strep, L. garvieae, PZR

Proje Numarası

2014.103.02.3

Kaynakça

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