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Current Approaches to Short and Long-Term Storage of Honey Bee Semen

Yıl 2022, Cilt: 7 Sayı: 1, 72 - 75, 31.03.2022
https://doi.org/10.35229/jaes.1008199

Öz

There are many studies on the storage of honey bee semen. Due to its unique structure, bee semen varies according to the storage period but is suitable for short-term storage conditions. However, in terms of long-term storage conditions, developments in mammals have not been caught. It is known that more studies are needed on this subject. The great advantages of semen storage are its effective role in eliminating the problems of inbreeding after a while in pure breed enterprises, increasing the yield characteristics and preventing gene pollution. To solve these problems, beekeepers have to buy breeding queens from abroad with very high costs, and the expected results cannot always be obtained from the bees brought. Especially considering the genetic risks faced by regional breeds in Turkey, it is necessary to preserve semen, to protect colonies in pure breed enterprises, as well as to develop genetically yield characteristics and to prevent inbreeding. It can also prevent the spread of diseases compared to natural mating by contributing to artificial insemination. By improving the storage conditions of honey bee semen, long-term use of semen from well-known colonies will be possible. Producers will be able to benefit from the advantages of artificial insemination at a cheaper cost, with the semen being sent to distant regions as in mammals under appropriate storage conditions. The purpose of this review is to give information about the storage conditions of honeybee semen.

Kaynakça

  • Abdelkader, F. B., Kairo, G., Bonnet, M., Barbouche, N., Belzunces, L. P., & Brunet, J. L. (2019). Effects of clothianidin on antioxidant enzyme activities and malondialdehyde level in honey bee drone semen. Journal of Apicultural Research, 58(5), 740-745.
  • Almeida, R., & Espencer Egea Soares, A. (2002). Usage of green coconut water and different tissue culture media for in vitro honey bee semen storage (Apis mellifera; Hymenoptera: Apoidea). Interciencia, 27(6), 317-321.
  • Cobey, S. W. (2007). Comparison studies of instrumentally inseminated and naturally mated honey bee queens and factors affecting their performance. Apidologie, 38(4), 390-410.
  • Cobey, S. W., Tarpy, D. R., & Woyke, J. (2013). Standard methods for instrumental insemination of Apis mellifera queens. Journal of Apicultural Research, 52(4), 1-18.
  • Collins, A. M. (2000). Survival of honey bee (Hymenoptera: Apidae) spermatozoa stored at above-freezing temperatures. Journal of economic entomology, 93(3), 568-571.
  • Çakmak, S., & Nur, Z. (2015). Effects of various cryoprotective agents on post-thaw drone semen quality
  • Dadkhah, F., Nehzati-Paghaleh, G., Zhandi, M., Emamverdi, M., & Hopkins, B. K. (2016). Preservation of honey bee spermatozoa using egg yolk and soybean lecithin-based semen extenders and a modified cryopreservation protocol. Journal of Apicultural Research, 55(4), 279-283.
  • den Boer, S. P., Boomsma, J. J., & Baer, B. (2009). Honey bee males and queens use glandular secretions to enhance sperm viability before and after storage. Journal of Insect Physiology, 55(6), 538-543.
  • Gül, A., Şahinler, N., Onal, A. G., Hopkins, B. K., & Sheppard, W. S. (2017). Effects of diluents and plasma on honey bee (Apis mellifera L.) drone frozen-thawed semen fertility. Theriogenology, 101, 109-113.
  • Harbo, J. R. (1979). Storage of honeybee spermatozoa at− 196° C. Journal of Apicultural Research, 18(1), 57-63.
  • Harbo, J. R. (1983). Survival of honey bee (Hymenoptera: Apidae) spermatozoa after two years in liquid nitrogen (–196 C). Annals of the Entomological Society of America, 76(5), 890-891.
  • Harbo, J. R., & Williams, J. L. (1987). Effect of above-freezing temperatures on temporary storage of honeybee spermatozoa. Journal of apicultural research, 26(1), 53-55.
  • Hopkins, B. K., & Herr, C. (2010). Factors affecting the successful cryopreservation of honey bee (Apis mellifera) spermatozoa. Apidologie, 41(5), 548-556.
  • Hopkins, B. K., Herr, C., & Sheppard, W. S. (2012). Sequential generations of honey bee (Apis mellifera) queens produced using cryopreserved semen. Reproduction, Fertility and Development, 24(8), 1079-1083.
  • Moritz, R.F.A. (1984) The effect of different diluents oninsemination success in the honeybee using mixedsemen. J. Apic. Res. 23(3), 164–167.
  • Lensky, Y., Eytan, B.-D. & Schindler, H. (1979) Ultrastructurc of the spermatozoon of the mature drone honeybee. Journal of Apicultural Research, 18, 264-271.
  • Lino-Neto, J., Báo, S. N., & Dolder, H. (2000). Sperm ultrastructure of the honey bee (Apis mellifera)(L)(Hymenoptera, Apidae) with emphasis on the nucleus-flagellum transition region. Tissue and Cell, 32(4), 322-327.
  • Locke, S. J., & Peng, Y. S. (1993). The effects of drone age, semen storage and contamination on semen quality in the honey bee (Apis mellifera). Physiological entomology, 18(2), 144-148.
  • Lodesani, M., Balduzzi, D., & Galli, A. (2004). Functional characterisation of semen in honeybee queen (Am ligustica S.) spermatheca and efficiency of the diluted semen technique in instrumental insemination. Italian Journal of Animal Science, 3(4), 385-392.
  • Paillard, M., Rousseau, A., Giovenazzo, P., & Bailey, J. L. (2017). Preservation of domesticated honey bee (Hymenoptera: Apidae) drone semen. Journal of economic entomology, 110(4), 1412-1418.
  • Taylor, M. A., Guzmán-Novoa, E., Morfin, N., & Buhr, M. M. (2009). Improving viability of cryopreserved honey bee (Apis mellifera L.) sperm with selected diluents, cryoprotectants, and semen dilution ratios. Theriogenology, 72(2), 149-159.
  • Wegener, J., & Bienefeld, K. (2012). Toxicity of cryoprotectants to honey bee semen and queens. Theriogenology, 77(3), 600-607.
  • Wegener, J., May, T., Kamp, G., & Bienefeld, K. (2014a). A successful new approach to honeybee semen cryopreservation. Cryobiology, 69(2), 236-242.
  • Wegener, J., May, T., Kamp, G., & Bienefeld, K. (2014b). New methods and media for the centrifugation of honey bee (Hymenoptera: Apidae) drone semen. Journal of economic entomology, 107(1), 47-53.

Bal Arısı Spermasının Kısa ve Uzun Süreli Saklanmasıyla İlgili Güncel Yaklaşımlar

Yıl 2022, Cilt: 7 Sayı: 1, 72 - 75, 31.03.2022
https://doi.org/10.35229/jaes.1008199

Öz

Bal arısı spermasının saklanabilmesiyle ilgili pek çok çalışma bulunmaktadır. Arı sperması kendine özgü yapısı nedeniyle saklama süresine göre değişmekle birlikte kısa süreli saklama koşulları için elverişlidir. Ancak uzun süreli saklama koşulları açısından memeli hayvanlardaki gelişmeler yakalanamamıştır. Bu konuda daha fazla çalışmaya ihtiyaç olduğu bilinmektedir. Spermanın saklanabilmesinin büyük avantajları saf ırk bulunan işletmelerde bir süre sonra akrabalı yetiştiriciliğin verdiği problemlerin giderilebilmesi, verim özelliklerinin arttırılabilmesi ve gen kirliliğinin önlenebilmesindeki etkin rolüdür. Arı yetiştiricileri bu problemleri giderebilmek için yurt dışından çok yüksek maliyetlerle damızlık kraliçe satın almak zorunda kalmakta ve getirilen arılardan her zaman beklenen sonuçlar alınamamaktadır. Özellikle ülkemizde bölgesel ırkların karşılaştığı genetik riskleri düşünürsek spermanın saklanabilmesi, saf ırk işletmelerdeki kolonilerin korunabilmesinin yanında genetik olarak verim özelliklerinin geliştirilmesi, akrabalı yetiştiriciliğin önlenebilmesi için de gereklidir. Ayrıca suni tohumlamaya katkıda bulunarak doğal çiftleşmeyle karşılaştırıldığında hastalıkların yayılmasını önleyebilmektedir. Bal arısı spermasının saklanma koşullarının geliştirilmesiyle özellikleri bilinen kaliteli kolonilerden alınan spermanın uzun süreli kullanımı mümkün olacaktır. Üreticiler alınan spermanın uygun saklama koşullarında memeli hayvanlardaki gibi uzak bölgelere gönderilebilmesiyle birlikte daha ucuza suni tohumlamanın sunduğu avantajlardan yararlanılabileceklerdir. Bu derlemenin amacı bal arası spermasının saklanma koşullarıyla ilgili bilgi vermektir.

Kaynakça

  • Abdelkader, F. B., Kairo, G., Bonnet, M., Barbouche, N., Belzunces, L. P., & Brunet, J. L. (2019). Effects of clothianidin on antioxidant enzyme activities and malondialdehyde level in honey bee drone semen. Journal of Apicultural Research, 58(5), 740-745.
  • Almeida, R., & Espencer Egea Soares, A. (2002). Usage of green coconut water and different tissue culture media for in vitro honey bee semen storage (Apis mellifera; Hymenoptera: Apoidea). Interciencia, 27(6), 317-321.
  • Cobey, S. W. (2007). Comparison studies of instrumentally inseminated and naturally mated honey bee queens and factors affecting their performance. Apidologie, 38(4), 390-410.
  • Cobey, S. W., Tarpy, D. R., & Woyke, J. (2013). Standard methods for instrumental insemination of Apis mellifera queens. Journal of Apicultural Research, 52(4), 1-18.
  • Collins, A. M. (2000). Survival of honey bee (Hymenoptera: Apidae) spermatozoa stored at above-freezing temperatures. Journal of economic entomology, 93(3), 568-571.
  • Çakmak, S., & Nur, Z. (2015). Effects of various cryoprotective agents on post-thaw drone semen quality
  • Dadkhah, F., Nehzati-Paghaleh, G., Zhandi, M., Emamverdi, M., & Hopkins, B. K. (2016). Preservation of honey bee spermatozoa using egg yolk and soybean lecithin-based semen extenders and a modified cryopreservation protocol. Journal of Apicultural Research, 55(4), 279-283.
  • den Boer, S. P., Boomsma, J. J., & Baer, B. (2009). Honey bee males and queens use glandular secretions to enhance sperm viability before and after storage. Journal of Insect Physiology, 55(6), 538-543.
  • Gül, A., Şahinler, N., Onal, A. G., Hopkins, B. K., & Sheppard, W. S. (2017). Effects of diluents and plasma on honey bee (Apis mellifera L.) drone frozen-thawed semen fertility. Theriogenology, 101, 109-113.
  • Harbo, J. R. (1979). Storage of honeybee spermatozoa at− 196° C. Journal of Apicultural Research, 18(1), 57-63.
  • Harbo, J. R. (1983). Survival of honey bee (Hymenoptera: Apidae) spermatozoa after two years in liquid nitrogen (–196 C). Annals of the Entomological Society of America, 76(5), 890-891.
  • Harbo, J. R., & Williams, J. L. (1987). Effect of above-freezing temperatures on temporary storage of honeybee spermatozoa. Journal of apicultural research, 26(1), 53-55.
  • Hopkins, B. K., & Herr, C. (2010). Factors affecting the successful cryopreservation of honey bee (Apis mellifera) spermatozoa. Apidologie, 41(5), 548-556.
  • Hopkins, B. K., Herr, C., & Sheppard, W. S. (2012). Sequential generations of honey bee (Apis mellifera) queens produced using cryopreserved semen. Reproduction, Fertility and Development, 24(8), 1079-1083.
  • Moritz, R.F.A. (1984) The effect of different diluents oninsemination success in the honeybee using mixedsemen. J. Apic. Res. 23(3), 164–167.
  • Lensky, Y., Eytan, B.-D. & Schindler, H. (1979) Ultrastructurc of the spermatozoon of the mature drone honeybee. Journal of Apicultural Research, 18, 264-271.
  • Lino-Neto, J., Báo, S. N., & Dolder, H. (2000). Sperm ultrastructure of the honey bee (Apis mellifera)(L)(Hymenoptera, Apidae) with emphasis on the nucleus-flagellum transition region. Tissue and Cell, 32(4), 322-327.
  • Locke, S. J., & Peng, Y. S. (1993). The effects of drone age, semen storage and contamination on semen quality in the honey bee (Apis mellifera). Physiological entomology, 18(2), 144-148.
  • Lodesani, M., Balduzzi, D., & Galli, A. (2004). Functional characterisation of semen in honeybee queen (Am ligustica S.) spermatheca and efficiency of the diluted semen technique in instrumental insemination. Italian Journal of Animal Science, 3(4), 385-392.
  • Paillard, M., Rousseau, A., Giovenazzo, P., & Bailey, J. L. (2017). Preservation of domesticated honey bee (Hymenoptera: Apidae) drone semen. Journal of economic entomology, 110(4), 1412-1418.
  • Taylor, M. A., Guzmán-Novoa, E., Morfin, N., & Buhr, M. M. (2009). Improving viability of cryopreserved honey bee (Apis mellifera L.) sperm with selected diluents, cryoprotectants, and semen dilution ratios. Theriogenology, 72(2), 149-159.
  • Wegener, J., & Bienefeld, K. (2012). Toxicity of cryoprotectants to honey bee semen and queens. Theriogenology, 77(3), 600-607.
  • Wegener, J., May, T., Kamp, G., & Bienefeld, K. (2014a). A successful new approach to honeybee semen cryopreservation. Cryobiology, 69(2), 236-242.
  • Wegener, J., May, T., Kamp, G., & Bienefeld, K. (2014b). New methods and media for the centrifugation of honey bee (Hymenoptera: Apidae) drone semen. Journal of economic entomology, 107(1), 47-53.
Toplam 24 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Makaleler
Yazarlar

Arda Onur Özkök 0000-0001-9932-3608

Yayımlanma Tarihi 31 Mart 2022
Gönderilme Tarihi 11 Ekim 2021
Kabul Tarihi 23 Aralık 2021
Yayımlandığı Sayı Yıl 2022 Cilt: 7 Sayı: 1

Kaynak Göster

APA Özkök, A. O. (2022). Current Approaches to Short and Long-Term Storage of Honey Bee Semen. Journal of Anatolian Environmental and Animal Sciences, 7(1), 72-75. https://doi.org/10.35229/jaes.1008199

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