Immunity-Related Enzyme Gene Interactions in Archocentrus centrarchus Infected with Lactococcus garvieae

Yıl 2023, Cilt: 8 Sayı: 3, 449 - 455, 30.09.2023

Ertuğrul Kankaya , Şükrü Önalan

https://doi.org/10.35229/jaes.1304779

Cited By: 1

Öz

Unsuitable environmental conditions and low host resistance in the aquatic environment are the most important factors in fish diseases. Bacterial diseases in fish can be treated with different preparations. The aim of this study is to determine the disease symptoms, expression levels of TNF and IL genes and some biochemical parameter changes in Archocentrus centrarchus by using herbal extract for the treatment of the disease caused by Lactococcus garvieae bacteria. Gene expression was performed by Real-Time PCR and enzyme activities were determined by spectrophotometric analysis method. It was observed that the expression level of TNF gene was 2.5 times more effective in the antibiotic (oxytetracycline) group and 14 times more effectively in the extract application. It was found that the antibiotic group had a close expression level compared to the control at the IL gene expression level, while the extract group expressed 5.5 times more. Glutathione s-transferase activity in the extract group increased significantly compared to the control and antibiotic groups. Superoxide dismutase activity was significantly higher in the extract group compared to the antibiotic and bacterial groups. It can be said that oxidative stress caused by bacterial infection is tried to be eliminated more in the extract group. It was determined that the andiz root extract which at the patent stage, which was used for the first time in the treatment of A. centrarchus fish infected with L. garvieae, one of the most important bacterial agents in the field of aquaculture, is applicable.

Anahtar Kelimeler

Cichlid fish, fish diseases, gene expression, IL, TNF

Lactococcus garvieae ile Enfekte Archocentrus centrarchus’ta Bağışıklıkla İlişkili Enzim Gen Etkileşimleri

Öz

Akuatik ortamda olumsuz çevre koşulları ve düşük konakçı direnci, balık hastalıklarda en önemli etkenlerdir. Balıklardaki bakteriyel hastalıklar farklı preparatlarla tedavi edilebilir. Bu çalışmanın amacı, Archocentrus centrarchus balığında Lactococcus garvieae bakterisinin neden olduğu hastalığın tedavisi için bitkisel öz kullanarak hastalık semptomlarını, TNF ve IL genlerinin ekspresyon seviyelerini ve bazı biyokimyasal parametre değişikliklerini belirlemektir. Gen ekspresyonu Real-Time PCR ile gerçekleştirilmiştir. Enzim aktiviteleri spektrofotometrik analiz yöntemi ile belirlenmiştir. TNF geninin ekspresyon seviyesinin antibiyotik (oksitetrasiklin) grubunda 2,5 kat, ekstrakt uygulamasında ise 14 kat daha etkili olduğu gözlenmiştir. Antibiyotik grubunun IL gen ifadesi düzeyinde kontrole yakın bir ifade düzeyine sahip olduğu, ekstrakt grubunun ise 5,5 kat daha fazla ifade ettiği tespit edilmiştir. Ekstrakt grubundaki glutatyon s-transferaz aktivitesi, kontrol ve antibiyotik gruplarına kıyasla önemli ölçüde artmıştır. Süperoksit dismutaz aktivitesi, antibiyotik ve bakteri gruplarına göre ekstrakt grubunda önemli düzeyde yükselmiştir. Bakteriyel enfeksiyonun neden olduğu oksidatif stresin ekstrakt grubunda daha fazla giderilmeye çalışıldığı söylenebilir. Su ürünleri yetiştiriciliği alanında en önemli bakteriyel etkenlerden biri olan L. garvieae ile enfekte olmuş A. centrarchus balığının tedavisinde ilk kez kullanılan patent aşamasındaki andız kökü özünün uygulanabilir olduğu tespit edilmiştir.

Anahtar Kelimeler

Balık hastalıkları, çiklit balığı, gen ifadesi, IL, TNF

Kaynakça

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