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Antibacterial, Antioxidant, Antidiabetic Potentials and Chemical Composition of Nicotiana glauca Graham Leaf Extract

Yıl 2023, Cilt: 8 Sayı: 4, 700 - 706, 31.12.2023
https://doi.org/10.35229/jaes.1325678

Öz

The purpose of this study is to investigate the chemical composition and potentiality of Nicotiana glauca G. leaf extract taken from the Turkish Republic of Northern Cyprus (TRNC), such as antibacterial, antioxidant, and antidiabetic compounds. In February 2020, a leaf of N. glauca was gathered in the Kyrenia district of Northern Cyprus. Methanol was used to extract the leaf sample. The content of essential oils in the extract was determined using gas chromatography and a headspace system test. Several methods were used to investigate the extract's antibacterial, antioxidant activities, and antidiabetic potential. On all microorganisms examined, the leaf methanol extract of N. glauca had no antibacterial activity. Total phenolic and flavonoid content, 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, ferric reducing antioxidant power, metal chelating, and phosphomolybdenum assays were all active with the methanol extract. The extract showed no antidiabetic efficacy. Furthermore, the essential oil was not found in the extract's chemical composition. According to the findings, the presence of antioxidant components in N. glauca leaf extract can protect against the detrimental effects of free radicals. Its application in Northern Cyprus becomes even more crucial as a result of this potential feature.

Kaynakça

  • Abdel Rahman, S.M., Abd-Ellatif, S.A., Deraz, S.F. & Khalil, A.A. (2011). Antibacterial activity of some wild medicinal plants collected from western Mediterranean coast, Egypt: Natural alternatives for infectious disease treatment. African Journal of Biotechnology, 10(52), 10733-10743. DOI: 10.5897/AJB11.007
  • Aldesuquy, H.S., Mashaly, I.A., El-Aal, M.A. & Mahdee, B.A. (2018). Phytochemical constituents, antibacterial and antioxidant activities of some medicinal plants. Current Trends in Biomedical Engineering & Biosciences, 16(2), 0047-0055. DOI: 10.19080/CTBEB.2018.16.55933
  • Alghamdi, A.A. (2021). Phytoconstituents screening and antimicrobial activity of the invasive species Nicotiana glauca collected from Al-Baha region of Saudi Arabia. Saudi Journal of Biological Sciences, 28(3), 1544-1547. DOI: 10.1016/j.sjbs.2020.12.034
  • Alharthi, A.S., Abd-ElGawad, A.M. & Assaeed, A.M. (2021). Influence of the invasive shrub Nicotiana glauca Graham on the plant seed bank in various locations in Taif region, western of Saudi Arabia. Saudi Journal of Biological Sciences, 28, 360-370. DOI: 10.1016/j.sjbs.2020.10.014
  • Başyiğit, B., Sağlam, H., Köroğlu, K. & Karaaslan, M. (2020). Compositional analysis, biological activity, and food protecting ability of ethanolic extract of Quercus infectoria gall. Journal of Food Processing and Preservation, 44(9), 1-12. DOI: 10.1111/jfpp.14692
  • Blois, M.S. (1958). Antioxidant determinations by the use of stable free radical. Nature, 1199-1200. DOI: 10.1038/1811199a0
  • Bogdanovic, S., Mitic, B., Ruscic, M. & Dolina, K. (2006). Nicotiana glauca Graham (Solanaceae), a new invasive plant in Croatia. Acta Botanica Croatica, 65, 203-209.
  • Cherif, A., Ammar, S. & Boukhchina, S. (2019). Composition and characterization by GC-MS of the essential oil extracted from Nicotiana glauca Graham. Grasas Y Aceites, 70(3), 1-5. DOI: 10.3989/gya.0927182
  • CLSI. (2012). Performance standards for antimicrobial disk susceptibility tests; approved standard, (11th ed.), CLSI document M02-A11, Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087, USA, 58p.
  • Dinis, T.C., Madeira, V.M. & Almeida, L.M. (1994). Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and assay peroxyl radical scavengers. Archives of Biochemistry and Biophysics, 315(1), 161-169. DOI: /10.1006/abbi.1994.1485
  • DiTomaso, J.M., Kyser, G.B., Oneto, S.R., Wilson, R.G., Orloff, S.B., Anderson, L.W., Wright, S.D., Roncoroni, J.A., Miller, T.L., Prather, T.S., Ransom, C., Beck, K.G., Duncan, C., Wilson, K.A. & Mann, J.J. (2013). Weed control in natural areas in the Western United States. Weed Research and Information Center, University of California, 544 pp.
  • Güngör Bilen, Z. (2004). Antidiabetik etkili bitkilerde αglukozidaz inhibitörleri. Yüksek Lisans, İstanbul Üniversitesi, Fen Bilimleri Enstitüsü. İstanbul, Türkiye, 83s.
  • Hassan, H.E., Abd El-Hameed Turki, Z. & Nasr, E.A. (2014). Ecological and phytochemical studies on Nicotiana glauca from Egypt. The Egyptian Society of Experimental Biology, 10(1), 87-95.
  • Kasiotis, K.M., Evergetis, E., Papachristos, D., Vangelatou, O., Antonatos, S., Milonas, P., Harotounian, S.A. & Machera, K. (2020). An essay on ecosystem availability of Nicotiana glauca graham alkaloids: the honeybees case study. BMC Ecology, 20(57), 1-14. DOI: 10.1186/s12898-020- 00325-3
  • Kazeem, M.I., Ogungbe, S.M., Saibu, G.M. & Aboyade, O.M. (2014). In vitro study on the hypoglycemic potential of Nicotiana tabacum leaf extracts. A Journal of the Bangladesh Pharmacological Society, 9(2), 140-145. DOI: 10.3329/bjp.v9i2.17540
  • Massadeh, R.K., El-Elimat, T., Al-Gharaibeh, M., Tawaha, K. & Alali, F.Q. (2022). UPLC-HRESIMS and GC-MS analysis of the leaves of Nicotiana glauca. Acta Pharmaceutica, 72, 1-12. DOI: 10.2478/acph-2022-0001
  • Mortimer, C.L., Bramley, P.M. & Fraser, P.D. (2012). The identification and rapid extraction of hydrocarbons from Nicotiana glauca: A potential advanced renewable biofuel source. Phytochemistry Letters, 5, 455-458. DOI: 10.1016/j.phytol.2012.04.004
  • Najah, Z., Elsherif, K.M., Kawan, E. & Farah, N. (2015). Phytochemical screening and heavy metals contents of Nicotiana glauca plant. International Journal of Pharmacy & Pharmaceutical Research, 4(3), 82-91.
  • Ollerton, J., Watts, S., Connerty, S., Lock, J., Parker, L., Wilson, I., Schueller, S., Nattero, J., Cocucci, A.A., Izhaki, I., Geerts, S., Pauw, A. & Stout, J.C. (2012). Pollination ecology of the invasive tree tobacco Nicotiana glauca: Comparisons across native and non-native ranges. Journal of Pollination Ecology, 9(12):85-95. DOI: 10.26786/1920- 7603(2012)12
  • Oyaizu, M. (1986). Studies on products of browning reactionAntioxidative activities of products of browning reaction prepared from glucosamine. The Japanese Journal of Nutrition and Dietetics, 44(6), 307-315. DOI: 10.5264/eiyogakuzashi.44.307
  • Owusu, E., Ahorlu, M.M., Afutu, E., Akumwena, A. & Asare, G.A. (2021). Antimicrobial activity of selected medicinal plants from a Sub-Saharan African Country against bacterial pathogens from post-operative wound infections. Medical Sciences, 9(2), 23:1-16. DOI: 10.3390/medsci9020023
  • Rinez, A., Daami-Remadi, M., Omezzine, F., Ladhari, A., Rinez, I. & Haouala, R. (2012). Assessment of the antifungal activity of Nicotiana glauca Graham aqueous and organic extracts against some pathogenic and antagonistic fungi. African Journal of Microbiology Research, 6(229), 4655-4661. DOI: 10.5897/AJMR11.979
  • Saliha, B., Khellaf, R., Wafa, H. & Fatiha, M. (2018). Bioactivity of Nicotiana glauca Graham (Solanaceae) and its toxic effects on Culiseta longiareolata (Diptera; Culicidae), International Journal of in Ayurveda and Pharmcy, 9(1), 123-126. DOI: 10.7897/2277-4343.09124
  • Sevindik, B. (2020). Stability of volatile compounds of Turkish saffron (Crocus sativus) after one-year storage. Journal of Raw Materials to Processed Foods, 1(2), 72-79.
  • Sharma, S. & Vig, A.P. (2013). Evaluation of in vitro antioxidant properties of methanol and aqueous extracts of Parkinsonia aculeata l. leaves. The Scientific World Journal, 1, 2-7. DOI: /10.1155/2013/604865
  • Stanković, M.S (2011). Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum l. extracts. Kragujevac Journal of Science, 33, 63-72.
  • Tabana, Y.M., Dahham, S.S., Ahmed Hassan, L.E., AlMansoub, M.A., Taleb-Agha, M. & Abdul Majid Amin, M.S. (2015). In vitro anti-metastatic and antioxidant activity of Nicotiana glauca fraction against breast cancer cells. Advances in Biological Research 9(2), 95-102. DOI: 10.5829/idosi.abr.2015.9.2.9521
  • Trifa, W., Akkal, S., Lefahal, M., Benmekhebi, L. & Khennouf, S. (2020). Preliminary screening of Nicotiana glauca extracts for determination of antioxidant activity by different methods. Current Issues in Pharmacy and Medical Sciences, 33(1), 32-37. DOI: 10.2478/cipms-2020-0007
  • Ucan Turkmen, F. & Mercimek Takci, H.A. (2018). Ultraviolet-C and ultraviolet-B lights effect on black carrot (Daucus carota ssp. sativus) juice. Journal of Food Measurement and Characterization, 12, 1038- 1046. DOI: 10.1007/s11694-018-9719-2
  • Zengin, G., Sarikurkcu, C., Aktumsek, A. & Ceylan, R. (2014). Sideritis galatica Bornm.: A source of multifunctional agents for the management of oxidative damage, Alzheimer’s’s and diabetes mellitus. Journal of Functional Foods, 11, 538-547. DOI: 10.1016/j.jff.2014.08.011.

Nicotiana glauca Graham Yaprak Ekstraktının Antibakteriyel, Antioksidan, Antidiyabetik Potansiyelleri ve Kimyasal Bileşimi

Yıl 2023, Cilt: 8 Sayı: 4, 700 - 706, 31.12.2023
https://doi.org/10.35229/jaes.1325678

Öz

Bu çalışmanın amacı, Kuzey Kıbrıs Türk Cumhuriyeti'nden (KKTC) alınan Nicotiana glauca G. yaprak ekstraktının kimyasal bileşimini ve antibakteriyel, antioksidan ve antidiyabetik bileşikler gibi potansiyelini araştırmaktır. Şubat 2020'de Kuzey Kıbrıs'ın Girne ilçesinde N. glauca yaprağı toplandı. Yaprak örneğini ekstrakte etmek için metanol kullanıldı. Ekstrakttaki uçucu yağların içeriği, gaz kromatografisi ve headspace sistem testi kullanılarak belirlendi. Ekstraktın antibakteriyel, antioksidan aktivitelerini ve antidiyabetik potansiyelini araştırmak için çeşitli yöntemler kullanıldı. İncelenen tüm mikroorganizmalar üzerinde, N. glauca'nın yaprak metanol ekstraktının antibakteriyel aktivitesi yoktu. Toplam fenolik ve flavonoid içeriği, 1,1-difenil-2-pikrilhidrazil radikal süpürme aktivitesi, ferrik indirgeyici antioksidan gücü, metal şelatlama ve fosfomolibden deneylerinin tümü metanol ekstraktı ile aktifti. Ekstrakt antidiyabetik etkinlik göstermedi. Ayrıca, ekstraktın kimyasal bileşiminde uçucu yağ bulunmadı. Elde edilen bulgulara göre, N. glauca yaprak ekstraktında antioksidan bileşenlerin bulunması, serbest radikallerin zararlı etkilerine karşı koruma sağlayabiliyor. Bu potansiyel özelliği nedeniyle Kuzey Kıbrıs'ta uygulanması daha da önem kazanmaktadır.

Kaynakça

  • Abdel Rahman, S.M., Abd-Ellatif, S.A., Deraz, S.F. & Khalil, A.A. (2011). Antibacterial activity of some wild medicinal plants collected from western Mediterranean coast, Egypt: Natural alternatives for infectious disease treatment. African Journal of Biotechnology, 10(52), 10733-10743. DOI: 10.5897/AJB11.007
  • Aldesuquy, H.S., Mashaly, I.A., El-Aal, M.A. & Mahdee, B.A. (2018). Phytochemical constituents, antibacterial and antioxidant activities of some medicinal plants. Current Trends in Biomedical Engineering & Biosciences, 16(2), 0047-0055. DOI: 10.19080/CTBEB.2018.16.55933
  • Alghamdi, A.A. (2021). Phytoconstituents screening and antimicrobial activity of the invasive species Nicotiana glauca collected from Al-Baha region of Saudi Arabia. Saudi Journal of Biological Sciences, 28(3), 1544-1547. DOI: 10.1016/j.sjbs.2020.12.034
  • Alharthi, A.S., Abd-ElGawad, A.M. & Assaeed, A.M. (2021). Influence of the invasive shrub Nicotiana glauca Graham on the plant seed bank in various locations in Taif region, western of Saudi Arabia. Saudi Journal of Biological Sciences, 28, 360-370. DOI: 10.1016/j.sjbs.2020.10.014
  • Başyiğit, B., Sağlam, H., Köroğlu, K. & Karaaslan, M. (2020). Compositional analysis, biological activity, and food protecting ability of ethanolic extract of Quercus infectoria gall. Journal of Food Processing and Preservation, 44(9), 1-12. DOI: 10.1111/jfpp.14692
  • Blois, M.S. (1958). Antioxidant determinations by the use of stable free radical. Nature, 1199-1200. DOI: 10.1038/1811199a0
  • Bogdanovic, S., Mitic, B., Ruscic, M. & Dolina, K. (2006). Nicotiana glauca Graham (Solanaceae), a new invasive plant in Croatia. Acta Botanica Croatica, 65, 203-209.
  • Cherif, A., Ammar, S. & Boukhchina, S. (2019). Composition and characterization by GC-MS of the essential oil extracted from Nicotiana glauca Graham. Grasas Y Aceites, 70(3), 1-5. DOI: 10.3989/gya.0927182
  • CLSI. (2012). Performance standards for antimicrobial disk susceptibility tests; approved standard, (11th ed.), CLSI document M02-A11, Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087, USA, 58p.
  • Dinis, T.C., Madeira, V.M. & Almeida, L.M. (1994). Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and assay peroxyl radical scavengers. Archives of Biochemistry and Biophysics, 315(1), 161-169. DOI: /10.1006/abbi.1994.1485
  • DiTomaso, J.M., Kyser, G.B., Oneto, S.R., Wilson, R.G., Orloff, S.B., Anderson, L.W., Wright, S.D., Roncoroni, J.A., Miller, T.L., Prather, T.S., Ransom, C., Beck, K.G., Duncan, C., Wilson, K.A. & Mann, J.J. (2013). Weed control in natural areas in the Western United States. Weed Research and Information Center, University of California, 544 pp.
  • Güngör Bilen, Z. (2004). Antidiabetik etkili bitkilerde αglukozidaz inhibitörleri. Yüksek Lisans, İstanbul Üniversitesi, Fen Bilimleri Enstitüsü. İstanbul, Türkiye, 83s.
  • Hassan, H.E., Abd El-Hameed Turki, Z. & Nasr, E.A. (2014). Ecological and phytochemical studies on Nicotiana glauca from Egypt. The Egyptian Society of Experimental Biology, 10(1), 87-95.
  • Kasiotis, K.M., Evergetis, E., Papachristos, D., Vangelatou, O., Antonatos, S., Milonas, P., Harotounian, S.A. & Machera, K. (2020). An essay on ecosystem availability of Nicotiana glauca graham alkaloids: the honeybees case study. BMC Ecology, 20(57), 1-14. DOI: 10.1186/s12898-020- 00325-3
  • Kazeem, M.I., Ogungbe, S.M., Saibu, G.M. & Aboyade, O.M. (2014). In vitro study on the hypoglycemic potential of Nicotiana tabacum leaf extracts. A Journal of the Bangladesh Pharmacological Society, 9(2), 140-145. DOI: 10.3329/bjp.v9i2.17540
  • Massadeh, R.K., El-Elimat, T., Al-Gharaibeh, M., Tawaha, K. & Alali, F.Q. (2022). UPLC-HRESIMS and GC-MS analysis of the leaves of Nicotiana glauca. Acta Pharmaceutica, 72, 1-12. DOI: 10.2478/acph-2022-0001
  • Mortimer, C.L., Bramley, P.M. & Fraser, P.D. (2012). The identification and rapid extraction of hydrocarbons from Nicotiana glauca: A potential advanced renewable biofuel source. Phytochemistry Letters, 5, 455-458. DOI: 10.1016/j.phytol.2012.04.004
  • Najah, Z., Elsherif, K.M., Kawan, E. & Farah, N. (2015). Phytochemical screening and heavy metals contents of Nicotiana glauca plant. International Journal of Pharmacy & Pharmaceutical Research, 4(3), 82-91.
  • Ollerton, J., Watts, S., Connerty, S., Lock, J., Parker, L., Wilson, I., Schueller, S., Nattero, J., Cocucci, A.A., Izhaki, I., Geerts, S., Pauw, A. & Stout, J.C. (2012). Pollination ecology of the invasive tree tobacco Nicotiana glauca: Comparisons across native and non-native ranges. Journal of Pollination Ecology, 9(12):85-95. DOI: 10.26786/1920- 7603(2012)12
  • Oyaizu, M. (1986). Studies on products of browning reactionAntioxidative activities of products of browning reaction prepared from glucosamine. The Japanese Journal of Nutrition and Dietetics, 44(6), 307-315. DOI: 10.5264/eiyogakuzashi.44.307
  • Owusu, E., Ahorlu, M.M., Afutu, E., Akumwena, A. & Asare, G.A. (2021). Antimicrobial activity of selected medicinal plants from a Sub-Saharan African Country against bacterial pathogens from post-operative wound infections. Medical Sciences, 9(2), 23:1-16. DOI: 10.3390/medsci9020023
  • Rinez, A., Daami-Remadi, M., Omezzine, F., Ladhari, A., Rinez, I. & Haouala, R. (2012). Assessment of the antifungal activity of Nicotiana glauca Graham aqueous and organic extracts against some pathogenic and antagonistic fungi. African Journal of Microbiology Research, 6(229), 4655-4661. DOI: 10.5897/AJMR11.979
  • Saliha, B., Khellaf, R., Wafa, H. & Fatiha, M. (2018). Bioactivity of Nicotiana glauca Graham (Solanaceae) and its toxic effects on Culiseta longiareolata (Diptera; Culicidae), International Journal of in Ayurveda and Pharmcy, 9(1), 123-126. DOI: 10.7897/2277-4343.09124
  • Sevindik, B. (2020). Stability of volatile compounds of Turkish saffron (Crocus sativus) after one-year storage. Journal of Raw Materials to Processed Foods, 1(2), 72-79.
  • Sharma, S. & Vig, A.P. (2013). Evaluation of in vitro antioxidant properties of methanol and aqueous extracts of Parkinsonia aculeata l. leaves. The Scientific World Journal, 1, 2-7. DOI: /10.1155/2013/604865
  • Stanković, M.S (2011). Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum l. extracts. Kragujevac Journal of Science, 33, 63-72.
  • Tabana, Y.M., Dahham, S.S., Ahmed Hassan, L.E., AlMansoub, M.A., Taleb-Agha, M. & Abdul Majid Amin, M.S. (2015). In vitro anti-metastatic and antioxidant activity of Nicotiana glauca fraction against breast cancer cells. Advances in Biological Research 9(2), 95-102. DOI: 10.5829/idosi.abr.2015.9.2.9521
  • Trifa, W., Akkal, S., Lefahal, M., Benmekhebi, L. & Khennouf, S. (2020). Preliminary screening of Nicotiana glauca extracts for determination of antioxidant activity by different methods. Current Issues in Pharmacy and Medical Sciences, 33(1), 32-37. DOI: 10.2478/cipms-2020-0007
  • Ucan Turkmen, F. & Mercimek Takci, H.A. (2018). Ultraviolet-C and ultraviolet-B lights effect on black carrot (Daucus carota ssp. sativus) juice. Journal of Food Measurement and Characterization, 12, 1038- 1046. DOI: 10.1007/s11694-018-9719-2
  • Zengin, G., Sarikurkcu, C., Aktumsek, A. & Ceylan, R. (2014). Sideritis galatica Bornm.: A source of multifunctional agents for the management of oxidative damage, Alzheimer’s’s and diabetes mellitus. Journal of Functional Foods, 11, 538-547. DOI: 10.1016/j.jff.2014.08.011.
Toplam 30 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Ekoloji (Diğer)
Bölüm Makaleler
Yazarlar

Melis Sümengen Özdenefe 0000-0003-0804-9557

Aysun Mercimek Takcı 0000-0002-3388-1153

Fikret Büyükkaya Kayış 0000-0002-3890-1635

Erken Görünüm Tarihi 25 Aralık 2023
Yayımlanma Tarihi 31 Aralık 2023
Gönderilme Tarihi 11 Temmuz 2023
Kabul Tarihi 15 Aralık 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 8 Sayı: 4

Kaynak Göster

APA Sümengen Özdenefe, M., Mercimek Takcı, A., & Büyükkaya Kayış, F. (2023). Antibacterial, Antioxidant, Antidiabetic Potentials and Chemical Composition of Nicotiana glauca Graham Leaf Extract. Journal of Anatolian Environmental and Animal Sciences, 8(4), 700-706. https://doi.org/10.35229/jaes.1325678


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