Biological activity of Laurus nobilis L. Leaf and Fruit Extract

Yıl 2024, Cilt: 9 Sayı: 3, 430 - 436, 30.09.2024

Ülkü Zeynep Üreyen Esertaş , Merve Cora

https://doi.org/10.35229/jaes.1456851

Öz

The leaves and fruits of Laurus nobilis L. are used in pharmaceutical applications with their various activities such as antioxidant, antimicrobial and anti-inflammatory. In current study, it was aimed to investigate antimicrobial, antiquorum sensing, cytotoxicity and antiviral activity of Laurus nobilis L. leaf and fruit extract prepared by %70 ethanol. This study was carried out in the Department of Medical Microbiology, Faculty of Medicine, Karadeniz Technical University, with Laurel leaves and fruits collected from Trabozon province in the Black Sea region. Antimicrobial activity was investigated by the agar well method. Gram negative, Gram positive bacteria and 2 fungi were used. Chromobacterium violaceum ATCC 12472 and Pseudomonas aeruginosa PAO1 strains were used for antiquorum sensing, antibiofilm and anti swarming activities. The cytotoxic effect of ethanol extract prepared from the leaf and fruit of Laurus nobilis L. plant on Vero, A549 and MDA-MB-231 cell lines was investigated by MTT method. The antiviral effect of the extracts on HSV-1 was investigated by MTT method. Antimicrobial and quorum sensing activity was determined to be moderate. It was understood that the leaf and fruit extracts of Laurus nobilis L. used in the study showed antiproliferative and antiviral effects in a dose-dependent manner. Laurel plant needs to be investigated in more detail using different solvents.

Anahtar Kelimeler

Cytotoxicity, HSV-1, Antimicrobial, Quorum sensing

Etik Beyan

YOK

Destekleyen Kurum

YOK

Teşekkür

YOK

Laurus nobilis L. Yaprak ve Meyve Özütlerinin Biyolojik Aktivitesi

Öz

Laurus nobilis L.'nin yaprak ve meyveleri antioksidan, antimikrobiyal ve antiinflamatuar gibi çeşitli aktiviteleriyle farmasötik uygulamalarda kullanılmaktadır. Bu çalışmada %70 etanol ile hazırlanan Laurus nobilis L. yaprak ve meyve ekstraktının antimikrobiyal, antikuorum duyarlılığı, sitotoksisitesi ve antiviral aktivitesinin araştırılması amaçlandı. Bu çalışma Karadeniz bölgesinde yer alan Trabozon ilinden toplanan Defne yaprak ve meyveleri ile Karadeniz Teknik Üniversitesi Tıp Fakültesi Tıbbi mikrobiyoloji anabilim dalında gerçekleştirildi. Antimikrobiyal aktivite agar kuyucuk yöntemiyle araştırıldı. Gram negatif, Gram pozitif bakteriler ve 2 mantar kullanıldı. Chromobacterium violaceum ATCC 12472 ve Pseudomonas aeruginosa PAO1 suşları antiquorum algılama, antibiyofilm ve anti-swarming aktiviteleri için kullanıldı. Laurus nobilis L. bitkisinin yaprak ve meyvesinden hazırlanan etanol ekstraktının Vero, A549 ve MDA-MB-231 hücre hatları üzerindeki sitotoksik etkisi MTT yöntemi ile araştırıldı. Ekstraktların HSV-1 üzerindeki antiviral etkisi MTT yöntemiyle araştırıldı. Antimikrobiyal ve çekirdek algılama aktivitesinin orta düzeyde olduğu belirlendi. Araştırmada kullanılan Laurus nobilis L. yaprak ve meyve ekstraktlarının doza bağlı olarak antiproliferatif ve antiviral etki gösterdiği anlaşıldı. Defne bitkisinin farklı çözücüler kullanılarak daha detaylı araştırılması gerekmektedir.

Anahtar Kelimeler

Sitotoksisite, HSV-1, Antimikrobiyal, Quorum sensing

Kaynakça

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