Antibiofilm, Antidiabetic and Antioxidant Potentials of Vitis labrusca L. Skin Extracts

Yıl 2024, Cilt: 9 Sayı: 4, 590 - 597

Uğur Kardil , Zeynep Akar , Azer Özad Düzgün

https://doi.org/10.35229/jaes.1526167

Öz

This study examined the antioxidant, antimicrobial, antibiofilm, and α-glucosidase inhibitory activities and the total phenolic and flavonoid contents of the different solvent (methanol, 50:50% methanol:water, and water) extracts from Vitis labrusca L. skin parts. The 50:50 methanol:water extract exhibited the highest antioxidant activity, exhibiting 153 µM TEAC and 0.0947 mg/mL SC50, as determined by the ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging assays, respectively. Additionally, the data demonstrated that the 50:50 methanol:water extract of the skin part of V. labrusca exhibited a higher total phenolic content, with 141 µg/mL GAE. The α-glucosidase enzyme activity of the 50:50% methanol:water extract (IC₅₀; 0.103 mg/mL) was observed to be higher than that of the other solvent extracts. The MIC values of the 50:50% methanol:water, water and methanol extracts of skin part of V. labrusca was determined as 12.5, 25 and 6.25 mg/mL, aganist to clinical antibiotic resistance Acinetobacter baumanii respectively. The results of this study indicate that the methanol, water and 50:50% methanol:water extracts were found to reduce the biofilm-forming capacity of the Acinetobacter baumannii isolate by approximately 1.7, 1.6 and 1.3-fold, respectively. The findings of our investigation suggest that skin parts of V. labrusca may serve as a promising candidate for the prevention and treatment of diseases associated with oxidative damage and bacterial infections. The results show that the components found in the waste skin extracts of these genotypes can be evaluated in terms of antioxidant, antidiabetic and antibacterial properties.

Anahtar Kelimeler

Antibiofilm, antioxidant activity, α-glucosidase, Vitis labrusca

Etik Beyan

This study, which we present as an article in the Journal of Anatolian Environmental and Animal Sciences, has collected the data and samples used together; that we have conducted or conducted experiments and analyzes in the relevant laboratories; that we have received information from other articles or books completely in the text and bibliography; we declare that we act in accordance with scientific research and ethical rules in the course of the study, and in the case of the contrary, we accept all kinds of legal results.

Destekleyen Kurum

None

Vitis Labrusca L. Kabuk Ekstraktlarının Antibiyofilm, Antidiyabetik ve Antioksidan Potansiyelleri

Öz

Bu çalışmada, Vitis labrusca L. bitkisinin kabuk kısımlarından elde edilen farklı çözücü (metanol, %50:50 metanol:su ve su) ekstraktlarının antioksidan, antimikrobiyal, antibiyofilm ve α-glukozidaz inhibitör aktiviteleri ile toplam fenolik ve flavonoid içerikleri incelendi. 50:50 metanol:su özütü, sırasıyla demir indirgeyici antioksidan gücü (FRAP) ve 2,2-difenil-1-pikrilhidrazil (DPPH•) radikal süpürücü analizleriyle belirlendiği üzere, 153 µM TEAC ve 0,0947 mg/mL SC50 ile en yüksek antioksidan aktiviteyi gösterdi. Ayrıca veriler, V. labrusca kabuk kısmından elde edilen 50:50 metanol:su özütünün 141 µg/mL GAE ile daha yüksek toplam fenolik madde içeriğine sahip olduğunu göstermiştir. %50:50 metanol:su ekstraktının (IC₅₀; 0,103 mg/mL) α-glukozidaz enzim aktivitesinin diğer çözücü ekstraktlarından daha yüksek olduğu gözlendi. V. labrusca kabuk kısmının %50:50 metanol:su, su ve metanol özütlerinin MİK değerleri klinik antibiyotik dirençli Acinetobacter baumanii’ ye karşı sırasıyla 12,5, 25 ve 6,25 mg/mL olarak belirlendi. Bu çalışmanın sonuçları, metanol, su ve %50:50 metanol:su ekstraktlarının Acinetobacter baumannii izolatının biyofilm oluşturma kapasitesini sırasıyla yaklaşık 1,7, 1,6 ve 1,3 kat azalttığını göstermektedir. Araştırmamızın bulguları, V. labrusca kabuk kısımlarının oksidatif hasar ve bakteriyel enfeksiyonlarla ilişkili hastalıkların önlenmesi ve tedavisi için umut verici bir aday olabileceğini düşündürmektedir. Sonuçlar, bu genotiplerin atık deri özütlerinde bulunan bileşenlerin antioksidan, antidiyabetik ve antibakteriyel özellikler açısından değerlendirilebileceğini göstermektedir.

Anahtar Kelimeler

Antibiyofilm, antioksidan aktivite, α-glukosidaz, Vitis labrusca

Kaynakça

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