The Anti-Proliferative Activity of Lyophilised Medicinal Leech (Hirudo Verbana) Saliva Extract on Breast Cancer Cell Line (MCF-7)

Yıl 2024, Cilt: 9 Sayı: 4, 508 - 513, 31.12.2024

Özgen Tural , Hüseyin Ayhan

https://doi.org/10.35229/jaes.1541179

Öz

Cancer is the leading cause of death and morbidity globally and is the second leading cause of death after cardiovascular disease. Breast cancer is the most common cancer in women with a mortality rate of 18 per cent and this rate is increasing. Cancer treatments used in modern medicine can have negative side effects. Medical leech saliva contains anti-proteolytic, platelet aggregation inhibitors, anticoagulant enzymes and anti-metastasic agents as natural compounds with fewer side effects. In this study, it was aimed to investigate the anti-proliferation of different concentrations of lyophilised medicinal leech secretion extract on MCF-7 (breast cancer) cancer cell lines. For this purpose, cell viability test (XTT) was performed to determine the cytotoxicity after application of various concentrations of medical leech secretion to the cell lines for 24 and 48 hours and the percentage viability of cancer cells was determined at each leech secretion concentration. The IC50 value of leech secretion on MCF-7 cell line was calculated as 490.61 µg/ml at 24 hours and 407.04 µg/ml at 48 hours depending on time and dilution rate. It was observed that the anti-proliferation effect of leech secretion applied at different doses on cell viability in MCF-7 cancer cell line increased as the dose amount increased and the duration increased.

Anahtar Kelimeler

Cell proliferation, Hirudo verbana, Medicinal leech, MCF-7 (breast cancer), XTT

Etik Beyan

Salgı alınan canlı Hirudo verbana omurgasız hayvan olduğundan etik kurul iznine gerek yoktur. Ayrıca hiçbir canlıya zarar verilmemiştir.

Teşekkür

The authors would like to thank Kübranur ÜNAL and Mustafa GÜNGÖRMÜŞ for their help in laboratory studies and support of the study. We also thank Fatma ÇORUK for checking the English of the article. We thank AYBÜ MERLAB for their support in all techniques. All authors declare that they have no actual or potential competing financial interests.

Liyofilize Tıbbi Sülük (Hirudo Verbana) Tükürük Ekstraktının Meme Kanseri Hücre Hattı (MCF-7) Üzerindeki Anti-Proliferatif Aktivitesi

Öz

Kanser, küresel çapta ölüm ve hastalıkların önde gelen nedenidir ve kardiyovasküler hastalıklardan sonra ikinci önde gelen ölüm nedenidir. Meme kanseri kadınlarda en sık görülen kanser olup ölüm oranı yüzde 18'dir ve giderek bu oran artmaktadır. Modern tıpta kullanılan kanser tedavilerinde olumsuz yan etkiler ortaya çıkabilmektedir. Tıbbi sülük tükürüğü daha az yan etkiye sahip doğal bileşikler olarak anti-proteolitik, trombosit agregasyon inhibitörleri, antikoagülan enzimler ve anti-metastazik ajanlar içermektedir. Bu çalışmada, liyofilize tıbbi sülük salgısı ekstraktının farklı konsantrasyonlarının MCF-7 (meme kanseri) kanser hücre hatları üzerindeki anti-proliferasyon araştırılması amaçlanmıştır. Bu amaçla, tıbbi sülük salgısının çeşitli konsantrasyonlarının hücre hatlarına 24 ve 48 saat süreyle uygulanmasının ardından sitotoksisiteyi belirlemek için hücre canlılık testi (XTT) yapılmış ve her bir sülük salgısı konsantrasyonunda kanser hücrelerinin yüzde canlılığı belirlenmiştir. Sülük salgısının MCF-7 hücre hattı üzerindeki IC50 değeri, zamana ve seyreltme oranına bağlı olarak 24 saatte 490,61 µg/ml ve 48 saatte 407,04 µg/ml olarak hesaplanmıştır. Farklı dozlarda uygulanan sülük salgısının MCF-7 kanser hücre hattında hücre canlılığı üzerindeki anti-proliferasyon etkisinin doz miktarı arttıkça ve süre uzadıkça arttığı gözlenmiştir.

Anahtar Kelimeler

Hirudo verbena, Hücre proliferasyonu, MCF-7 (meme kanseri), Tıbbi sülük, XTT

Kaynakça

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