Effects of Propolis on the Development Stages and Biochemical Composition of Musca domestica LİNNAEUS, 1758 (Diptera: Muscidae)

Yıl 2024, Cilt: 9 Sayı: 4, 641 - 647, 31.12.2024

Melisa Samur , Meltem Kökdener

https://doi.org/10.35229/jaes.1570185

Öz

The housefly (Musca domestica L.) is well known a global pest of animals and humans. The houseflies contain high purity chitin and protein which are widely used in industry, and medicine. Their larvae can produce animal protein in the biodegradation of organic waste . House flies provide an alternative for recycling nutrients while also generating multiple income streams, so their large-scale production is important. In this study, the effects of different doses of propolis applied to a wheat bran diet on the developmental stages (larval and pupal development time, larval, pupal, and adult weight, and larval, pupal, and adult survival) and the protein, carbohydrate, and lipid content in Musca domestica larvae were examined under laboratory conditions (62±0.2% humidity and 25.06 ± 0.8°C temperature). Thirty Musca domestica larvae were reared on substrates exposed to four different propolis concentrations. One-way ANOVA was used to compare life history and biochemical parameters. The results showed that increasing concentrations of propolis reduced larval length and weight. While a decrease in the number of pupae and adults was observed, a significant increase in pupal weight was noted. Larval development time was not affected by propolis diets compared to the control, but pupal development time was shortened. In biochemical composition, no significant difference in protein content in Musca domestica larvae was observed with increasing propolis concentrations. However, compared to the control, increasing propolis concentrations increased carbohydrate content and decreased lipid content in Musca domestica larvae.

Anahtar Kelimeler

Biochemical composition, Developmental stages, Housefly (Musca domestica), Propolis

Propolisin Musca domestica LİNNAEUS, 1758 (DİPTERA: MUSCİDAE) Sineğinin Gelişim Evreleri ve Biyokimyasal Kmpozisyonu Üzerine Etkileri

Öz

Ev sineği (Musca domestica L.), hayvanlar ve insanlar için küresel bir zararlı olarak bilinir. Ev sinekleri, endüstride ve tıpta yaygın olarak kullanılan yüksek saflıkta kitin ve protein içerir. Larvaları, organik atıkların biyolojik olarak parçalanmasında hayvansal protein üretebilir. Ev sinekleri, besin maddelerinin geri dönüştürülmesi için bir alternatif sunarken aynı zamanda birden fazla gelir akışı da yaratır, bu nedenle büyük ölçekli üretimleri önemlidirBu çalışmada; propolisin farklı dozlarının buğday kepeği diyetine uygulanmasıyla Musca domestica sineğinin gelişim evreleri (larva ve pupa gelişim süresi, larva, pupa ve yetişkin ağırlığı, larva pupa ve yetişkin hayatta kalma) ve larvalardaki protein karbonhidrat ve lipit miktarlarına etkisi laboratuvar koşullarında (%62±0.2 nem ve 25.06 ± 0.8°C sıcaklık) incelenmiştir. Bu çalışmada; 30 adet Musca domestica larvası dört farklı propolis konsantrasyonuna maruz bırakılmış besiyeri üzerinde yetiştirilmiştir. Yaşam öyküsü ve biyokimyasal parametrelerini karşılaştırmak için tek yönlü varyans analizi (ANOVA) kullanılmıştır. Çalışma sonucunda; propolisin artan konsantrasyonlarında, larva uzunluğu ve ağırlığını azalttığı gösterilmiştir. Pupa ve erişkin sayılarında azalma görülmüşken, pupa ağırlığında anlamlı bir artma gösterilmiştir. Larval gelişim süresi kontrole kıyasla propolisli diyetlerden etkilenmediği fakat pupal gelişim süresini kısalttığı gösterilmiştir. Biyokimyasal komposizyonda ise; propolisin artan konsantrasyonlarında Musca domestica larvalarındaki protein miktarları arasında anlamlı bir fark görülmemiştir. Ancak; propolisin artan konsantrasyonlarında kontrole kıyasla Musca domestica larvalarındaki karbonhidrat miktarını arttırmıştır, lipit miktarı ise azalmıştır.

Anahtar Kelimeler

Biyokimyasal kompozisyon, Ev sineği (Musca domestica), Gelişim evreleri, Propolis.

Kaynakça

• Almeida, E.D. & Menezes, H. (2002). Anti-inflammatory activity of propolis extracts: a review. Journal of Venomous Animals and Toxins, 8(2), 191-212.
• Ararso, Z., & Legesse, G. (2016). Insecticidal action of honeybees propolis extract against larvae of lesser wax moth. Agrıculture and Bıology Journal of North Amerıca, 7(6), 302-306.
• Bakaaki, N., Aanyu, M., Onen, H., Opio, D., Sengendo, F. & Chemurot, M. (2023). Growth performance of the black soldier fly, Hermetia illucens larvae (Linnaeus, 1758) fed on honeybee propolis-treated wheat bran. Journal of Insects as Food and Feed, 9(3), 281-287.
• Cammack, J.A. & Tomberlin, J.K. (2017). The impact of diet protein and carbohydrate on select life-history traits of the black soldier fly Hermetia illucens (L.)(Diptera: Stratiomyidae). Insects, 8(2), 56.
• Denli, M., Cankaya, S., Silici, S., Okan, F. & Uluocak, A.N., 2005. Effects of dietary addition of Turkish propolis on growth performance, carcass characteristics and serum variables of quail (Coturnix cortunix japonica). Asian-Australasian journal of Animal Science, 18(16).
• Ganda, H., Zannou, E.T., Kenis, M., Abihona, H.A., Houndonougbo, F.M., Chrysostome, C.A.A. M., ... & Mensah, G. A. (2022). Effect of four rearing substrates on the yield and the chemical composition of housefly larvae, Musca domestica L. 1758 (Diptera: Muscidae). International Journal of Tropical Insect Science, 1-9.
• Garedew, A., Schmolz, E., & Lamprecht, I. (2004). Effect of the bee glue (propolis) on the calorimetrically measured metabolic rate and metamorphosis of the greater wax moth Galleria mellonella. Thermochimica Acta, 413(1-2), 63-72.
• Garedew, A., Schmolz, E., Schricker, B. & Lamprecht, I. (2002). Microcalorimetric investigation of the action of propolis on Varroa destructor mites. Thermochimica acta, 382(1-2), 211-220.
• Geckil, H., Ates, B., Durmaz, G., Erdogan, S. & Yilmaz, I. (2005). Antioxidant, free radical scavenging and metal chelating characteristics of propolis.
• Geden, C.J., Nayduch, D., Scott, J.G., Burgess IV, E.R., Gerry, A.C., Kaufman, P.E. & Machtinger, E.T. (2021). House fly (Diptera: Muscidae): biology, pest status, current management prospects, and research needs. Journal of Integrated Pest Management, 12(1), 39.
• Gekker, G., Hu, S., Spivak, M., Lokensgard, J.R. & Peterson, P.K. (2005). Anti-HIV-1 activity of propolis in CD4+ lymphocyte and microglial cell cultures. Journal of ethnopharmacology, 102(2), 158-163.
• Ghisalberti, E.L. (1979). Propolis: a review. Bee world, 60(2), 59-84.
• Greenberg, B. (2019). Flies and disease: I. Ecology, classification, and biotic associations.
• Gülhan, M.F. (2009). Pestisite maruz bırakılmış gökkuşağı alabalık dokularında propolisin etkilerinin incelenmesi. Yüksek Lisans Tezi, NÜ Fen Bilimleri Enstitüsü, Niğde.
• Hepşen, İ. F., Tilgen, F. & Er, H. (1996). Propolis: Tıbbi özellikleri ve oftalmolojik kullanımı. Journal of Turgut Ozal Medical Center, 3(4).
• Hwangbo, J., Hong, E.C., Jang, A., Kang, H.K., Oh, J.S., Kim, B.W. & Park, B.S. (2009). Utilization of house fly-maggots, a feed supplement in the production of broiler chickens. Journal of Environmental Biology, 30(4)
• Ítavo, C.C.B.F., Morais, M.G., Costa, C., Ítavo, L.C.V., Franco, G.L., Da Silva, J.A. & Reis, F.A. (2011). Addition of propolis or monensin in the diet: Behavior and productivity of lambs in feedlot. Animal Feed Science and Technology, 165(3-4), 161-166.
• Kacaniova, M., Rovna, K., Arpasova, H., Cubon, J., et al., (2012). In vitro and in vivo activity of propolis on the microbiota from gastrointestinal tract of chickens. Journal of Environmental Science and health part A Toxic Harzadous Substances and Environmental Engineering, 47(11), 1665-1671.
• Kinasih, I., Julita, U., Suryani, Y., Cahyanto, T., Annisa, D.S., Yuliawati, A. & Putra, R.E. (2018). Addition of black soldier fly larvae (Hermetia illucens L.) and propolis to broiler chicken performance. In IOP Conference Series: Earth and Environmental Science, November 2018, (Vol. 187, No. 1, p. 012026). IOP Publishing.
• Kovtunova, A., Drevko, Y., Faust, E., Bannikova, A. & Larionova, O. (2018). Dynamics of amino acid profile of Musca domestica larva during cultivation on substrate enriched with microelements. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 88, 1257-1264.
• Kökdener, M. & Kiper, F. (2020). The impact of diet protein and carbohydrate on select life-history traits of the housefly Musca domestica Linnaeus, 1758 (Diptera: Muscidae). Munis Entomology Zoology, 15, 171- 179.
• Marruci, MC. (1995). Propolis, chemical composition, biological properties and therapeutic activity. Apidologie, 26, 83-9.
• Nirala, S. K. & Bhadauria, M. (2008). Propolis reverses acetaminophen induced acute hepatorenal alterations: a biochemical and histopathological approach. Archives of Pharmacal Research, 31, 451- 461.
• Scott, J.G., Liu, N., Kristensen, M. & Clark, A.G. (2009). A case for sequencing the genome of Musca domestica (Diptera: Muscidae). Journal of medical entomology, 46(2), 175-182
• Sforcin, J.M. (2016). Biological properties and therapeutic applications of propolis. Phytotherapy research, 30(6), 894-905.
• Simone-Finstrom, M., Borba, R. S., Wilson, M. & Spivak, M. (2017). Propolis counteracts some threats to honey bee health. Insects, 8(2), 46.
• Skidmore P. (1985). The biology of the Muscidae of the world. Ser Entomol. 29, 1-550.
• Subha, G., Indira, P. & Kunit, K.M., (2010). Application and effectiveness of immunestimulants, prebiotics and probiotics in Aquaculture. The Israeli journal of Aquaculture-Bamidgeh, 62(3), 130-138
• Tognocchi, M., Abenaim, L., Adamaki-Sotiraki, C., Athanassiou, G.C., Rumbos, I.C., Mele, M., Conti, B. & Conte. G. (2024). Effect of different diet composition on the fat profile of two different black soldier fly larvae populations. Animal, 18, 101205
• Touzani, S., Imtara, H., Katekhaye, S., Mechchate, H., Ouassou, H., Alqahtani, A. S., & Lyoussi, B. (2021). Determination of phenolic compounds in various propolis samples collected from an African and an Asian region and their impact on antioxidant and antibacterial activities. Molecules, 26(15), 4589.
• Yılmaz, Y. & Gündüz, N.E.A. (2021). Kadmiyumun küçük balmumu güvesi larvalarında protein, lipit ve karbohidrat miktarları ile etkileşimi. Anadolu Tarım Bilimleri Dergisi, 36(2), 326-333.
• Zafarnejad, K., Nazar, A. & Mostafa, R. (2017). Effect of bee glue on growth performance and immune response of broiler chickens. Journal of applied animal research. 459(1), 280-284.
• Zhang, Z., Wang, H., Zhu, J., Suneethi, S. & Zheng, J. (2012). Swine manure vermicomposting via housefly larvae (Musca domestica): the dynamics of biochemical and microbial features. Bioresource Technology, 118, 563-571.