Sağlıklı ve Epileptik Köpeklerde Fekal Mikrobiyotanın Karşılaştırmalı Değerlendirmesi

Yıl 2025, Cilt: 10 Sayı: 1, 79 - 86

Nilgün Aksoy , Şeyda Yaman , Alper Çiftci

https://doi.org/10.35229/jaes.1598473

Öz

İdiyopatik epilepsi, hipotez edilen genetik yatkınlıktan başka bir nedenin tanımlanamadığı, tekrarlayan nöbetlerle karakterize, köpekleri ve kedileri etkileyen nörolojik bir durumdur. Bu çalışma sağlıklı köpeklerin ve epilepsi tanısı almış köpeklerin fekal florasındaki mezofilik aerobik bakteri, koliform bakteri, Escherichia coli, Enterococcus spp., Staphylococcus aureus ve Lactobacillus spp. içeriğini karşılaştırmalı olarak değerlendirmek amacıyla gerçekleştirildi. Çalışma materyali olarak 10 sağlıklı ve 10 epilepsi tanısı almış köpeğin dışkısı kullanıldı. Seçici izolasyon ve sayımlar, dışkı örneklerinin mezofilik aerobik bakteriler için Tryptic Soy Agar, koliform bakteriler için MacConkey Agar, E. coli için Eosin Metilen Mavisi Agar, Enterococcus spp. için Bile Eskülin Agar, S. aureus için Mannitol Salt Agar ve Lactobacillus spp. için De Man, Rogosa ve Sharpe Agar'a ekilmesiyle gerçekleştirildi. Sağlıklı köpeklerin dışkı florasında mezofilik aerobik bakteri, koliform bakteri, E. coli, Enterococcus spp., S. aureus ve Lactobacillus spp. sayıları sırasıyla 6,6x106, 2,6x105, 1,4x105, 1,6x105, 3,2x104 ve 4,1 x 104 kob/mg ve epilepsi tanısı konulan köpeklerde sırasıyla 9,0x106, 5,0x105, 2,8x105, 6,4x105, 6,3x104 ve 1,2x104 kob /mg olarak bulundu. Sonuç olarak, epilepsili köpeklerin dışkı florasında Lactobacillus spp. sayısında sağlıklı köpeklere kıyasla bir azalma görülürken, diğer bakterilerin sayısında artış görüldü. Epileptik köpeklerin diyetine probiyotik bakteri eklenmesinin bağırsak mikrobiyotasını düzenlemeye ve nöbetlerin şiddetini ve sıklığını azaltmaya yardımcı olabileceği kanısına varıldı.

Anahtar Kelimeler

dışkı florası, idiyopatik epilepsi, köpek

Etik Beyan

ÇALIŞMA KAPSAMINDA ETİK KURUL İZNİNE GEREK YOKTUR.

Destekleyen Kurum

Scientific Research Projects Commission of Ondokuz Mayıs University

Proje Numarası

PYO.VET.1904. 22.028

Comparative Assessment of Faecal Microbiota in Healthy and Epileptic Dogs

Öz

Idiopathic epilepsy is a neurological condition affecting dogs and cats characterized by repeated seizures for which no cause other than a hypothesized genetic predisposition can be identified. The aim of this study was to comparatively evaluate the content of mesophilic aerobic bacteria, coliform bacteria, Escherichia coli, Enterococcus spp., Staphylococcus aureus and Lactobacillus spp. in the faecal flora of healthy dogs and dogs diagnosed with epilepsy. Feces of 10 healthy dogs and 10 dogs diagnosed with epilepsy were used as study material. Selective isolation and counts were performed with inoculating the faecal samples were on Tryptic Soy Agar for mesophilic aerobic bacteria, MacConkey Agar for coliform bacteria, Eosin Methylene Blue Agar for E. coli, Bile Esculin Agar for Enterococcus spp., Mannitol Salt Agar for S. aureus and De Man, Rogosa and Sharpe Agar for Lactobacillus spp. The numbers of mesophilic aerobic bacteria, coliform bacteria, E. coli, Enterococcus spp., S. aureus and Lactobacillus spp. were 6.6x106, 2.6x105, 1.4x105, 1.6x105, 3.2x104 and 4.1 x 104 cfu/mg in the faecal flora of healthy dogs; and 9.0x106, 5.0x105, 2.8x105, 6.4x105, 6.3x104, and 1.2x104 cfu/mg in dogs diagnosed with epilepsy, respectively. As a result, there was a decrease in the number of Lactobacillus spp. in the faecal flora of dogs with epilepsy compared to healthy dogs, while the number of other bacteria increased. It was concluded that adding probiotic bacteria to the diet of epileptic dogs could help regulate the gut microbiota and reduce the severity and frequency of seizures.

Anahtar Kelimeler

dog, faecal flora, idiopathic epilepsy

Proje Numarası

PYO.VET.1904. 22.028

Kaynakça

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