Developing Lucilia sericata flies on natural and artificial substrates containing natural ingredients

Yıl 2025, Cilt: 10 Sayı: 2, 143 - 150

Nevra Polat , Salih Mollahaliloğlu , Murat Koç

https://doi.org/10.35229/jaes.1608450

Öz

Foods with high protein and organic content are effective in the maturation of sperm and eggs of Calliphoridae and mating. In Lucilia sericata fly colony continuity, liver food in the procedure in terms of both chemical properties and protein and moisture content is a suitable food substrate for females to lay their eggs. However, liver or any other animal tissue can cause contamination due to decomposition during larval development of the eggs. Many sterilised artificial diets have been developed for controlled mass rearing. In this study, natural diet liver and liver-agar artificial diets containing natural diet were preferred after spawning. In particular, the effects of these diets on the following characteristics of L sericata were analysed: 1) growth and development time 2) larval stage length and weight 3) pupal and adult length 4) morphological appearance of larvae, pupae and adults. The experiment was conducted in two replicate experiments with six study groups in each. In general, rearing on liver-based media was similar, but growth and development on sterile liver-agar media was more efficient than on liver and no contamination occurred. There was a significant difference (p<0.05) between measurements of first instar larval length, first instar larval weight, second instar larval weight, third instar larval length, third instar larval weight, pupal length and adult length of L. sericata larvae fed on liver and liver-agar. However, there was no significant difference between the second instar larval length measurements of L. sericata larvae fed with liver-agar and liver (p>0.05).

Anahtar Kelimeler

Artificial substrate, growth and development, larval feeding, Lucilia sericata

Teşekkür

We thank Ankara Yıldırım Beyazıt University entomology researchers suggestions on an earlier draft of this manuscript. We also thank laborotory technicians for their helps in laboratory studies.

Lucilia sericata sineklerinin doğal ve doğal maddeler içeren substratlar üzerinde geliştirilmesi

Öz

Calliphoridae üyelerinin sperm ve yumurtalarının olgunlaşmasıyla çiftleşmesinde protein oranı yüksek, organik içerikli besinler etkilidir. Lucilia sericata sinek koloni sürekliliğinde hem kimyasal özelliği hem de protein ve nem içeriği bakımından prosedürdeki ciğer besini dişilerin yumurtalarını bırakmasında uygun besin substratıdır. Ancak yumurtaların larval gelişiminde ciğer veya herhangi bir hayvansal doku çürümeye bağlı olarak kontaminasyona sebep olabilir. Kontrollü kitlesel yetiştirme için birçok sterilize yapay diyet geliştirilmiştir. Bu çalışmada yumurtlamayı takiben doğal diyet ciğer ile doğal diyet içerikli ciğer-agar yapay diyet tercih edildi. Özellikle bu besin ortamlarında Lucilia sericata’nın şu özellikleri üzerindeki etkileri incelendi: 1) büyüme ve gelişme zamanları 2) larval dönemsel evre boy ve ağırlıkları 3) pupa ve ergin boyları 4) larva, pupa ve ergin morfolojik görünüm. Deney her birinde altışar çalışma grupları ile tekrar teknik bulunan iki deneme ile sürdürüldü. Genel olarak ciğer bazlı besin ortamlarında yetiştirme benzerdi ancak steril ciğer-agar ortamında büyüme ve gelişim ciğerdeki gelişme kıyasla daha etkili idi ve kontaminasyon gelişmedi. Lucilia sericata larvalarından ciğer ve ciğer-agar ile beslenen birinci dönem larva boy, birinci dönem larva ağırlık, ikinci dönem larva ağırlık, üçüncü dönem larva boy, üçüncü dönem larva ağırlık, pupaya geçiş dönemindeki boy ve ergin dönemindeki boy ölçümleri arasında anlamlı fark elde edilmiştir (p<0,05). Ancak ciğer-agar ve ciğer ile beslenen Lucilia sericata larvalarının ikinci dönem larva boy ölçümleri arasında fark yoktur (p>0,05).

Anahtar Kelimeler

Büyüme ve gelişme, larva beslenmesi, Lucilia sericata, yapay substrat

Kaynakça

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