İkinci Derece Yanıkların Tedavisinde Tıbbi Bitkiler İçeren Kremlerin Etkinliği: Biyokimyasal ve Histopatolojik Araştırma

Yıl 2025, Cilt: 10 Sayı: 5, 693 - 702

Seda Aşkın , Esra Palabıyık , Handan Uğuz Bayrakçeken , Bahri Avcı , Ayşe Nurseli Sulumer , Harika Derya Tamer , Hakan Aşkın

https://doi.org/10.35229/jaes.1659982

Öz

Yanık yaralanmaları, artan oksidatif stres ve bozulmuş doku bütünlüğü ile karakterize edilen ciddi patofizyolojik durumları temsil eder ve etkili terapötik müdahaleleri gerektirir. Bu çalışma, Juglans regia yaprak özütü, Argania spinosa çekirdeği yağı, Prunus dulcis yağı ve Lavandula angustifolia yağı içeren yeni bir krem formülasyonunun ikinci derece yanık yaraları üzerindeki biyokimyasal ve histopatolojik etkilerini değerlendirir. Bir Wistar-albino sıçan modeli kullanılarak, formülasyonun terapötik potansiyelini belirlemek için süperoksit dismutaz (SOD) ve malondialdehit (MDA) dahil olmak üzere oksidatif stres belirteçleri histopatolojik değerlendirmelerle birlikte analiz edildi. Sonuçlar, tedavi grubunda SOD seviyelerinde önemli bir artış ve MDA seviyelerinde eş zamanlı bir azalma olduğunu göstererek, gelişmiş bir antioksidan tepkisi ve azalmış lipid peroksidasyonunu düşündürmektedir. Histopatolojik değerlendirmeler, yanık kontrol ve gümüş sülfadiazin gruplarına kıyasla hızlandırılmış epitelizasyon, artmış fibroblast aktivitesi ve iyileştirilmiş keratinizasyon ortaya koydu. Bu bulgular, test edilen formülasyonun doku rejenerasyonunu desteklerken oksidatif hasarı etkili bir şekilde azalttığını göstermektedir. Geleneksel yanık tedavilerine potansiyel bir alternatif olarak, bu bitki bazlı formülasyon ikinci derece yanıkları yönetmek için yenilikçi ve uygun maliyetli bir terapötik strateji sunabilir. Ancak, insan uygulamalarında etkinliğini doğrulamak için daha fazla klinik çalışmaya ihtiyaç vardır.

Anahtar Kelimeler

Antioksidan aktivite , bitkisel yanık tedavisi , histopatolojik değerlendirme

Etik Beyan

Bu çalışma Atatürk Üniversitesi Tıbbi Deneysel Uygulama ve Araştırma Merkezi (ATADEM) Etik Kurulunun 26.12.2024 tarihli, E-75296309-050.01.04-2400427040 sayılı kararına uygun olarak yürütülmüştür.

Teşekkür

Deneysel çalışmalarımızın gerçekleştirildiği, ATADEM'e, Fen Fakültesi Moleküler Biyoloji ve Genetik Laboratuvarına ve Tıp Fakültesi Tıbbi Patoloji Laboratuvarı yetkililerine teşekkür ederiz.

Effectiveness of Cream Containing Medicinal Plants in the Treatment of Second Degree Burns: Biochemical and Histopathological Investigation

Öz

Burn injuries represent severe pathophysiological conditions characterized by increased oxidative stress and impaired tissue integrity, necessitating effective therapeutic interventions. This study evaluates the biochemical and histopathological effects of a novel cream formulation containing Juglans regia leaf extract, Argania spinosa kernel oil, Prunus dulcis oil, and Lavandula angustifolia oil on second-degree burn wounds. Using a Wistar-albino rat model, oxidative stress markers, including superoxide dismutase (SOD) and malondialdehyde (MDA), were analyzed alongside histopathological assessments to determine the formulation’s therapeutic potential. The results demonstrated a significant increase in SOD levels and a concurrent reduction in MDA levels in the treatment group, suggesting an enhanced antioxidant response and reduced lipid peroxidation. Histopathological evaluations revealed accelerated epithelialization, increased fibroblast activity, and improved keratinization compared to the burn control and silver sulfadiazine groups. These findings indicate that the tested formulation effectively mitigates oxidative damage while promoting tissue regeneration. As a potential alternative to conventional burn treatments, this plant-based formulation may offer an innovative and cost-effective therapeutic strategy for managing second-degree burns. However, further clinical studies are required to confirm its efficacy in human applications.

Anahtar Kelimeler

Antioxidant activity , herbal burn therapy , histopathological evaluation

Etik Beyan

This study was conducted in accordance with the decision of the Atatürk University Medical Experimental Application and Research Center (ATADEM) Ethics Committee, numbered E-75296309-050.01.04-2400427040 and dated 26.12.2024.

Teşekkür

We would like to thank ATADEM, the Faculty of Science Molecular Biology and Genetics Laboratory, and the Faculty of Medicine Medical Pathology Laboratory where our experimental studies were carried out.

Kaynakça

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